시금장, 청국장 및 표고버섯을 혼합 발효한 제품의 이화학 및 기능적 특성
Physicochemical and functional characteristics of fermented products by using Sigumjang, Cheonggukjang and oak mushroom
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초록
본 연구의 목적은 청국장, 시금장, 표고버섯 분말의 첨가비율을 달리하여 새로운 발효 식품(일명: 청금장)을 개발하고자 하였다. 시금장, 청국장, 시금장과 청국장의 첨가비율을 달리하여 청금장 A(Sigumjang:Cheonggukjang=1:2), 청금장 B(Sigumjang:Cheonggukjang=1:1), 청금장 C(Sigumjang: Cheonggukjang=2:1) 시료를 제조한 후 이화학적 특성과 기능성 평가를 실시하였다. 청금장은 시금장보다 단백질 함량은 증가하였으며, 청국장보다 지방과 칼로리는 낮았으나, 유리당 함량은 청금장 C에서 5.8681 g/100g으로 가장 높은 함량을 나타내었다. DPPH 라디컬 소거능은 시금장과 청금장 C가 양성대조구인 BHT와 유사할 정도로 높은 활성이 나타났다. SOD 유사활성능은 시금장이 가장 높은 SOD 유사활성능을 나타내었고 청금장 중에서는 청금장 C가 가장 높았다. 금속이온의 지방산패 촉진에 대한 청금장의 억제능은 Cu2+ ion에 대한 억제능보다 Fe2+ ion에 대한 억제능이 다소 높은 것으로 나타났고, 활성 산소종의 지방산패 촉진에 대한 청금장의 억제능은 KO2보다 H2O2에 대한 억제능이 다소 높은 것으로 나타났다. Xanthine oxidase 활성저해능은 700 ppm 에서는 전반적으로 높은 xanthine oxidase 활성 저해능을 나타내었고, 특히 시금장과 청금장 C는 높은 xanthine oxidase 활성 저해능을 나타내었다. α-Glucosidase 저해 활성능은 모든 농도(300 ppm, 500 ppm, 700 ppm)에서 전반적으로 우수한 α-glucosidase 저해 활성능을 나타내었으며 시금장과 청금장 C는 높은 α-glucosidase 저해 활성능을 나타내어 혈당을 조절하는데 도움을 줄 것으로 기대된다. 관능평가 결과 청금장 C가 색, 향기, 쓴맛, 짠맛 및 종합적인 맛에서 가장 높은 평가을 받은 것으로 나타났기 때문에 시금장과 청국장을 단독으로 사용하는 것보다 시금장과 청국장을 2:1로 혼합하여 제품을 제조하는 것이 이화학적 특성과 기능성에 효과적일 것으로 판단된다.
Abstract
The purpose of this study was to develop a new fermented product (named as Cheonggeumjang) using Sigumjang, Cheonggukjang and Oak mushroom. This study was conducted to evaluate the effects of Sigumjang, Cheonggukjang, and Cheonggeumjang, which were mixed in a different ratio as A (Sigumjang: Cheonggukjang = 1:2), B (Sigumjang: Cheonggukjang = 1:1) and C (Sigumjang: Cheonggukjang = 2 : 1). Then, the functions and physicochemical properties of products were investigated. We found that the crude protein content in Cheonggeumjang was higher than in Sigumjang whereas fat and calories content was less than that of Cheonggukjang. Free sugar content in Cheonggeumjang C 5.8681 g/100g was the highest. Moroever, Cheonggeumjang C and Sigumjang has an antioxidant activities. The electron donating capacity, SOD like activity and the inhibitory effect on xanthine oxidase of these two were significantly high than other group. Fat rancidity is promoted in the presence of metal ion, Cheonggeumjang group has higher inhibitory effect on Fe2+ion than on Cu2+ ion. The rancidity of fat is also increased by reactive oxygens species, Cheonggeumjang group inhibited H2O2 in higher extent than KO2. Also, α-glucosidase inhibition activity of Cheonggeumjang C in all of the concentrations (300 ppm, 500 ppm and 700 ppm) is higher than other groups. In sensory evaluation, Cheonggeumjang C groups is ranked significantly higher than the other groups while considering color, flavor, taste and the overall acceptability. Taken together, the results of this study suggest that Cheonggeumjang is best ingredient for increasing the consumer acceptability and functionality.
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