저장 중 겉보리에 발생하는 곰팡이와 곰팡이독소 변화
Change of fungi and mycotoxin in hulled barley under different conditions and period
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초록
To establish good storage practices for hulled barley against mycotoxin contamination, we measured occurrence of fungi and mycotoxin in hulled barley grains under various storage conditions. Hulled barley grains collected from two places were stored in five different warehouses: 1) two without temperature control, 2) one with temperature controlled at 12℃, 3) a chamber set at 15℃/65% relative humidity, and 4) one seed storage set at 10℃. The samples were stored for six month with temperature and relative humidity monitored regularly. Every stored samples were retrieved after 0, 1, 3, and 6 month to investigate fungal and mycotoxin contamination. From the stored grains, Fusarium, Epicoccum, Alternaria, and Drechslera spp. were frequently detected. In the warehouses without temperature control, Fusarium and Alternaria spp. constantly decreased, whereas Drechslera spp. increased along with storage period. In the other warehouses with temperature controlled, Fusarium spp. decreased slowly and more than 2.5 log CFU/g of Fusarium spp. were detected after 6 month storage. The level of nivalenol was maintained during 0-3 month but increased after 6 month storage. There was no difference in the nivalenol levels between the warehouses. Therefore reducing storage period less than 6 months could be more effective to control nivalenol contamination in hulled barley grains.
Abstract
To establish good storage practices for hulled barley against mycotoxin contamination, we measured occurrence of fungi and mycotoxin in hulled barley grains under various storage conditions. Hulled barley grains collected from two places were stored in five different warehouses: 1) two without temperature control, 2) one with temperature controlled at 12℃, 3) a chamber set at 15℃/65% relative humidity, and 4) one seed storage set at 10℃. The samples were stored for six month with temperature and relative humidity monitored regularly. Every stored samples were retrieved after 0, 1, 3, and 6 month to investigate fungal and mycotoxin contamination. From the stored grains, Fusarium, Epicoccum, Alternaria, and Drechslera spp. were frequently detected. In the warehouses without temperature control, Fusarium and Alternaria spp. constantly decreased, whereas Drechslera spp. increased along with storage period. In the other warehouses with temperature controlled, Fusarium spp. decreased slowly and more than 2.5 log CFU/g of Fusarium spp. were detected after 6 month storage. The level of nivalenol was maintained during 0-3 month but increased after 6 month storage. There was no difference in the nivalenol levels between the warehouses. Therefore reducing storage period less than 6 months could be more effective to control nivalenol contamination in hulled barley grains.
참고문헌 (29)
- Alshannaq A, "Occurrence, toxicity, and analysis of major mycotoxins in food", Int J Environ Res Public Health, 14, pp.632~ (2017)
- Seung-Ho Lee, "Population Structure of Fusarium graminearum from Maize and Rice in 2009 in Korea", The Plant Pathology Journal, 26(4), pp.321~327 (2010), 한국식물병리학회
- Marin S, "Mycotoxins : Occurrence, toxicology, and exposure assessment", Food Chem Toxicol, 60, pp.218~237 (2013)
- Miller JD, "Fungi and mycotoxins in grain: Implications for stored product research", J Stored Prod Res, 31, pp.1~16 (1995)
- Taligoola HK, "Mycobiota and aflatoxins associated with imported rice grains stored in Uganda", Czech Mycol, 63, pp.93~107 (2011)
- Pinciroli M, "Mycobiota evolution during storage of paddy, brown and milled rice in different genotypes", Summa Phytopathol, 39, pp.157~161 (2013)
- Choi J, "Barley", pp.129~ (2013), Rural Development Administration
- Kim S, "Barley & Wheat, GAP production guide", National Institute of Agricultural Sciences, 23~24 (2014)
- 이데레사, "미곡종합처리장 곡류시료 내 곰팡이 오염과 Fusarium 독소 발생", 식물병 연구, 20(4), pp.289~294 (2014), 한국식물병리학회[DOI]
- Legzdina L, "Comparison of infection with Fusarium head blight and accumulation of mycotoxins in grain of hulless and covered barley", J Cereal Sci, 40, pp.61~67 (2004)
- Clear R, "The effect of hull removal and pearling on Fusarium species and trichothecenes in hulless barley", Can J Plant Sci, 77, pp.161~166 (1997)
- 함현희, "겉보리의 저장온도와 수분함량이 붉은곰팡이병균과 곰팡이독소 오염에 미치는 영향", 한국식품위생안전성학회지, 32(4), pp.321~328 (2017), 한국식품위생안전성학회
- Lee T, "Rapid detection method for fusaric acid-producing species of Fusarium by PCR", Res Plant Dis, 21, pp.326~329 (2015)
- White TJ, "PCR protocols: a guide to methods and applications", pp.315~322 (1990), Academic Press Inc
- O’Donnell K, "Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies", Proc Natl Acad Sci USA, 95, pp.2044~2049 (1998)
- van Wyk PS, "A selective medium for the isolation of Fusarium spp. from soil debris", Phytophylactica, 18, pp.67~69 (1986)
- Beattie S, "The effect of grain storage conditions on the viability of Fusarium and deoxynivalenol production in infested malting barley", J Food Prot, 61, pp.103~106 (1998)
- Birzele B, "Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters", Food Addit Contam, 17, pp.1027~1035 (2000)
- Choi S, "Behaviour of Aspergillus flavus and Fusarium graminearum on rice as affected by degree of milling, temperature, and relative humidity during storage", Food Microbiol, 46, pp.307~313 (2015)
- Ramirez ML, "Temperature and water activity effects on growth and temporal deoxynivalenol production by two Argentinean strains of Fusarium graminearum on irradiated wheat grain", Int J Food Microbiol, 106, pp.291~296 (2006)
- Schrodter R, "Influence of harvest and storage conditions on trichothecenes levels in various cereals", Toxicol Lett, 153, pp.47~49 (2004)
- Magan N, "Interactions between field, and storage fungi on wheat grain", Trans Br Mycol Soc, 85, pp.29~37 (1985)
- Homdork S, "Influence of different storage conditions on the mycotoxin production and quality of Fusarium-infected wheat grain", J Phytopathol, 148, pp.7~15 (2000)
- White NDG, "Effects of storage conditions on quality loss of hull-less and hulled oats and barley", Can J Plant Sci, 79, pp.475~482 (1999)
- Singh DB, "Effect of culture media, pH and temperature on growth behaviour of Alternaria brassicae and Drechslera graminea", Proc Indian natn Sci Acad, B46, pp.393~396 (1980)
- Wiewiora B, "Rhizoctonia solani and other fungi detected in perennial ryegrass seeds after harvest and storage", Phytopathol Pol, 26, pp.19~27 (2002)
- Kim JC, "Natural occurrence of Fusarium mycotoxins(trichothecenes and zearalenone)in barley and corn in Korea", Appl Environ Microbiol, 59, pp.3798~3802 (1993)
- 류재기, "2010년산 맥류의 붉은곰팡이병 발생 및 Fusarium 곰팡이독소 자연발생", 식물병 연구, 17(3), pp.272~279 (2011), 한국식물병리학회
- Zhang H, "Retention of deoxynivalenol and its derivatives during storage of wheat grain and flour", Food Control, 65, pp.177~181 (2016)
