포도 과피 및 종자의 탄닌 분석을 위한 추출 용매 조건 탐색
Solvent extraction conditions for the analysis of condensed tannins from grape skin and seeds
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본 연구에서는 포도 과피 및 종자에서 초음파 추출법을 통해 methanol, ethanol, acetone용매를 대상으로 각각 100%, 70%, 70% 용매에 0.5% HCl을 첨가한 조건에서 총 탄닌 함량 및 monomer와 dimer 탄닌 함량을 측정하였다. 과피의 총 탄닌 함량은 추출 용매 조건에 따라 0.01-2.92 mg/g으로 나타났으며 종자의 총 탄닌 함량은 8.82-14.72 mg/g 으로 나타났다. 그 결과, 총 탄닌 함량은 과피 및 종자에서 모두 70% acetone 조건에서 가장 우수한 검출량을 나타냈다. Monomer 및 dimer 탄닌 등 개별 탄닌 함량을 측정한 결과, 과피에서는 70% acetone이 가장 적합한 용매 조건으로 판단되었다. 종자에서는 70% acetone이 가장 높은 탄닌 검출량을 나타내었으나, monomer 탄닌 검출량이 70% methanol 조건과 유의차가 없었으며 dimer 탄닌이 70% methanol에서 높게 검출되어 70% methanol을 가장 적합한 용매 조건으로 판단하였다. 개별 탄닌에 대한 분석은 지금도 꾸준히 진행되고 있으며 분석 효율을 높이는 분석 방법에 대한 연구가 계속되어야 할 것으로 판단된다.
Abstract
The total tannin and individual condensed tannin concentrations in the fruit skin and seeds of “Campbell Early” grapes were analyzed by ultrasound-assisted extraction using various solvents (ethanol, methanol, and acetone, both pure and diluted to 70% with and without 0.5% HCl). The total tannin contents of the grape skin and seeds depended on the extraction-solvent conditions and varied between 0.01 and 2.92 mg/g for the skin, and 8.82 and 14.72 mg/g for the seeds. The most effective solvent for extracting total tannins from both the grape skin and seeds was found to be 70% acetone, which facilitated the detection of high concentrations of tannin monomers and dimers. Monomer tannins (catechin and gallocatechin) concentrations in grape seeds were detected at high levels using both 70% acetone and 70% methanol as the extraction solvents; however, dimer tannins (procyanidin B1 and procyanidin B2) were detected at high levels in grape seeds using 70% methanol. Hence, 70% methanol was effective for measuring the concentrations of condensed tannins in grape seeds. Since we are currently analyzing individual tannins, research into finding the analysis conditions most appropriate for individual situations is still required.
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