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Scopolamine 유발 건망증 마우스 모델에서 육미지황탕(六味地黃湯)의 기억력 개선 및 항산화 효과

Anti-amnesic Effect and Antioxidant Defense Systems of Yukmijihwang-tang on Scopolamine-induced Memory Impairment in Mice

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Objectives: The objective of this study was to observe the anti-amnesic effects of Yukmijihwang-tang (YMJHT), on the scopolamine (Sco)-induced memory impairment in C57BL/6 mice through its favorable acetylcholine (ACh). Also, to observe acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect. Methods: Six groups, with a total of 20 normal and 100 Sco treated mice were selected based on their body weights after 1 week of acclimatization, were used in this study as follows. Half of the mice in each group were used for passive avoidance task tests and hippocampus ACh content, AChE activity and ChAT mRNA expression measurement, and the remaining half in each group used for Morris water maze test and measurement of cerebral antioxidant defense system. Results: Amnesia due to AChE activations and destroyed cerebral cortex antioxidant defense systems were markedly and dose-dependently inhibited after 28 days of continuous oral pre-treatment with YMJHT 400, 200 and 100 mg/kg, respectively. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg. Conclusions: Based on the results, it was established that oral administration of YMJHT favorably alleviates Sco-induced memory impairment, through preservation of ACh, mediated by up-regulation of ChAT mRNA expressions and related AChE inhibition and augmentation of cerebral antioxidant defense system, at least in a condition of this experiment. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg.

Abstract

Objectives: The objective of this study was to observe the anti-amnesic effects of Yukmijihwang-tang (YMJHT), on the scopolamine (Sco)-induced memory impairment in C57BL/6 mice through its favorable acetylcholine (ACh). Also, to observe acetylcholinesterase (AChE) activity, Choline acetyltransferase (ChAT) mRNA expressions, and antioxidant effect. Methods: Six groups, with a total of 20 normal and 100 Sco treated mice were selected based on their body weights after 1 week of acclimatization, were used in this study as follows. Half of the mice in each group were used for passive avoidance task tests and hippocampus ACh content, AChE activity and ChAT mRNA expression measurement, and the remaining half in each group used for Morris water maze test and measurement of cerebral antioxidant defense system. Results: Amnesia due to AChE activations and destroyed cerebral cortex antioxidant defense systems were markedly and dose-dependently inhibited after 28 days of continuous oral pre-treatment with YMJHT 400, 200 and 100 mg/kg, respectively. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg. Conclusions: Based on the results, it was established that oral administration of YMJHT favorably alleviates Sco-induced memory impairment, through preservation of ACh, mediated by up-regulation of ChAT mRNA expressions and related AChE inhibition and augmentation of cerebral antioxidant defense system, at least in a condition of this experiment. The overall effects of YMJHT 400 mg/kg were similar to those of tacrine 10 mg/kg.

참고문헌 (46)

  1. Chuong NN, "Anti-amnesic effect of alkaloid fraction from Lycopodiella cernua (L.) Pic. Serm. on scopolamine-induced memory impairment in mice", Neurosci Lett, 575, pp.42~46 (2014)
  2. Becker R, "Potential pharmacotherapy of Alzheimer disease. A com -parison of various forms of physostigmine administration", Acta Neurol Scand Suppl, 116, pp.19~32 (1988)
  3. 최원희, "들깨 메탄올 추출물의 acetylcholinesterase 억제활성 및 세포독성 보호효과", 한국식품과학회지, 36(6), pp.1026~1031 (2004), 한국식품과학회
  4. Saxena G, "Effect of donepezil and tacrine on oxidative stress in intracerebral streptozotocin-induced model of dementia in mice", Eur J Pharmacol, 581(3), pp.283~289 (2008)
  5. Watkins PB, "Hepatotoxic effects of tacrine administration in patients with Alzheimer’s disease", JAMA, 271(13), pp.992~998 (1994)
  6. Xiao J, "Lactobacillus casei-01 facilitates the ameliorative effects of proanthocyanidins extracted from lotus seedpod on learning and memory impairment in scopolamine-induced amnesia mice", PLoS One, 9(11), pp.e112773~ (2014)
  7. Hasanein P, "Ameliorative effect of rosmarinic acid on scopolamine-induced memory impairment in rats", Neurosci Lett, 585, pp.23~27 (2015)
  8. Kang M, "Effects of Yukmijihwang-tang derivatives(YMJd)on ibotenic acid-induced amnesia in the rat", Biol Pharm Bull, 29(7), pp.1431~1435 (2006)
  9. Lee S, "Sulforaphane alleviates scopolamine-induced memory impairment in mice", Pharmacol Res, 85, pp.23~32 (2014)
  10. Nam Y, "Ameliorating effects of constituents from Cortex Acanthopanacis Radicis on memory impairment in mice induced by scopolamine", J Tradit Chin Med, 34(1), pp.57~62 (2014)
  11. Morris R, "Developments of a water-maze procedure for studying spatial learning in the rat", J Neurosci Methods, 11, pp.47~60 (1984)
  12. Jamall IS, "Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart: a possible mechanism of cad mium cardiotoxicity", Toxicol Appl Pharmacol, 80, pp.33~42 (1985)
  13. Sedlak J, "Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent", Anal Biochem, 25(1), pp.192~205 (1968)
  14. Aebi H, "Methods in Enzymatic Analysis", pp.673~686 (1974), Academic Press
  15. Sun Y, "A simple method for clinical assay of superoxide dismutase", Clin Chem, 34, pp.497~500 (1988)
  16. Levene A, "Pathological factors influencing excision of tumours in the head and neck. Part I", Clin Otolaryngol Allied Sci, 6(2), pp.145~151 (1981)
  17. Ludbrook J, "Update: microcomputer statistics packages. A personal view", Clin Exp Pharmacol Physiol, 24(3-4), pp.294~296 (1997)
  18. Kang SJ, "Fermentation with Aquilariae Lignum enhances the anti-diabetic activity of green tea in type II diabetic db", db mouse, 2014, pp.6(9), 3536~3571
  19. American Psychiatric Association, "Diagnostic and Statistical Manual of Mental Disorder" (1994), American Psychiatric Association
  20. LeDoux JE, "Emotional memory systems in the brain", Behav Brain Res, 58, pp.69~79 (1993)
  21. Park SM, "Tacrine, an oral acetylcholinesterase inhibitor, induced hepatic oxidative damage, which was blocked by liquiritigenin through GSK3-beta inhibition", Biol Pharm Bull, 38(2), pp.184~192 (2015)
  22. Song XY, "Effects of liuwei dihuang pill on erythrocyte aldose reductase activity in early diabetic nephropathy patients", Zhongguo Zhong Xi Yi Jie He Za Zhi, 24(12), pp.1087~1090 (2004)
  23. Kang DG, "Yukmijihwang-tang ameliorates ischemia", reperfusion-induced renal injury in rats, 2006, pp.104(1-2), 47~53
  24. Ha H, "Safety evaluation of Yukmijihwang-tang:Assessment of acute and subchronic toxicity in rats", Evid Based Complement Alternat Med, 2011, pp.672136~ (2011)
  25. Shin IS, "Inhibitory effect of Yukmijihwang-tang, a traditional herbal formula against testosterone-induced benign prostatic hyperplasia in rats", BMC Complement Altern Med, 12, pp.48~ (2012)
  26. Oh MS, "Yukmijihwangtang protects against cyclophosphamide-induced reproductive toxicity", Reprod Toxicol, 24(3-4), pp.365~370 (2007)
  27. Ki-Shuk Shim, "Yukmijihwang-tang Inhibits Receptor Activator for Nuclear Factor-κB Ligand–Induced Osteoclast Differentiation", Journal of Medicinal Food, 14(11), pp.1439~1447 (2011), 한국식품영양과학회
  28. Shen JJ, "The effect of liu-wei-di-huang wan on cytokine gene expression from human peripheral blood lymphocytes", Am J Chin Med, 31(2), pp.247~257 (2003)
  29. Abe E, "Reversal effect of DM-9384 on scopolamine-induced acetylcholine depletion in certain regions of the mouse brain", Psychopharmacology, 105(3), pp.310~316 (1991)
  30. Roof RL, "A comparison of long-term functional outcome after 2 middle cerebral artery occlusion models in rats", Stroke, 32(11), pp.2648~2657 (2001)
  31. Zambrzycka A, "Effects of aging and amyloid-beta peptides on choline acetyltransferase activity in rat brain", Neurochem Res, 27, pp.277~281 (2002)
  32. Mohapel P, "Forebrain acetylcholine regulates adult hippocampal neurogenesis and learning", Neurobiol Aging, 26(6), pp.939~946 (2005)
  33. Eichenbaum H, "How does the brain organize memories?", Science, 277(5324), pp.330~332 (1997)
  34. Giacobini E, "Cholinesterase inhibitors: new roles and therapeutic alternatives", Pharmacol Res, 50(4), pp.433~440 (2004)
  35. Wattanathorn J, "Zingiber officinale mitigates brain damage and improves memory impairment in focal cerebral ischemic rat", Evid Based Complement Alternat Med, 2011, pp.429505~ (2011)
  36. Rao KS, "Free radical induced oxidative damage to DNA : relation to brain aging and neurological disorders", Indian J Biochem Biophys, 46(1), pp.9~15 (2009)
  37. Ghumatkar PJ, "Nootropic, neuroprotective and neurotrophic effects of phloretin in scopolamine induced amnesia in mice", Pharmacol Biochem Behav, 135, pp.182~191 (2015)
  38. Kumar H, "Cognitive enhancing effects of alpha asarone in amnesic mice by influencing cholinergic and antioxidant defense mechanisms", Biosci Biotechnol Biochem, 76(8), pp.1518~1522 (2012)
  39. Nunomura A, "Oxidative damage is the earliest event in Alzheimer disease", J Neuropathol Exp Neurol, 60(8), pp.759~767 (2001)
  40. Mattson MP, "Cellular and molecular mechanisms underlying perturbed energy metabolism and neuronal degeneration in Alzheimer's and Parkinson's diseases", Ann N Y Acad Sci, 893, pp.154~175 (1999)
  41. Swerdlow RH, "Brain aging, Alzheimer's disease, and mitochondria", Biochim Biophys Acta, 1812, pp.1630~1639 (2011)
  42. Lee JS, "Hippocampal memory enhancing activity of pine needle extract against scopolamine-induced amnesia in a mouse model", Sci Rep, 5, pp.9651~ (2015)
  43. Mansoorali KP, "Cerebroprotective effect of Eclipta alba against global model of cerebral ischemia induced oxidative stress in rats", Phytomedicine, 19(12), pp.1108~1116 (2012)
  44. Raichle ME, "The pathophysiology of brain ischemia", Ann Neurol, 13(1), pp.2~10 (1983)
  45. Odabasoglu F, "Gastroprotective and antioxidant effects of usnic acid on indomethacin-induced gastric ulcer in rats", J Ethnopharmacol, 103(1), pp.59~65 (2006)
  46. Cheeseman KH, "An introduction to free radical biochemistry", Br Med Bull, 49(3), pp.481~493 (1993)

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