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튀니지 식물 11종 추출물의 HepG2 세포에서 산화적 스트레스 및 지질 축적 억제 효과

Inhibitory effects of Tunisian plants extracts on oxidative stress and lipid accumulation in HepG2 cells

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주제분류식품과학

초록

본 연구는 튀니지 식물 추출물 11종의 생리 효능을 체계적으로 밝히기 위한 기초 자료를 얻고자 HepG2 세포에서 식물 11종의 산화스트레스 및 지질 축적률 억제 효과를 검증하였다. 튀니지 식물 중 총폴리페놀 함량 및 총플라보노이드 함량은 각각 P. lentiscus L.과 N. glauca Graham이 가장 높았다. 튀니지식물 11종의 DPPH, ABTS, FRAP 및 ORAC를 통한 우수한 항산화 활성을 확인하였다. 지질 과산화물 함량 억제 효과는 R. officinalis L.과 T. capitatus Hoffmanns. & Link에서 가장 높았으며 quercetin 및 caffeic acid 성분에 의한 것임을 확인하였다. 11종 중 지질과산화물 함량 억제 효과가 높은 상위 5종의 HepG2 세포에서 ORO 염색법 및 중성 지방 함량을 측정한 결과, R. officinalis L.에서 oleic acid만 처리한 세포에 비해 25% 이상의 지질 축적률 및 중성지방 함량 억제 효과를 보였다. 이상의 결과로부터 본 논문이 튀니지 식물 11종의 생리활성물질, 항산화 활성 및 지질 축적 억제 활성을 검토하는 기초 자료로 활용될 수 있을 것이라 사료된다.

Abstract

This study was undertaken to evaluate the antioxidative and lipid accumulation inhibitory effects in HepG2 cell of 11 Tunisian plants extracts. Total phenolics contents (TPC), and total flavonoid contents (TFC) of 11 plants extracts were measured, and antioxidative activities was analyzed using DPPH, ABTS, FRAP, ORAC and TBA assay. Inhibitory effect of oxidative stress was evaluated by cell viability and lipid peroxidation level in H2O2-treated HepG2 cells. Lipid accumulation inhibitory effect was determined by Oil-Red-O staining and intracellular triglyceride assay in HepG2 cell. M. communis L. (156.73 mgGAE/g) and N. glauca Graham (108.81 mgNAE/g) were the highest TPC and TFC, respectively, among 11 plants. M. communis L. were the highest antioxidant activity in DPPH and ABTS. FRAP and ORAC results revealed that antioxidant activity in 10 species were higher than the positive control. Among the 11 species, 5 species with the lowest malondialdehyde level were selected and HPLC analysis revealed that plants contain caffeic acid, quercetin, and rutin. 5 plants treatment inhibited lipid peroxidation level and protected HepG2 cells from oxidative stress. Moreover 5 plants significantly inhibited the lipid accumulation and triglyceride content. These results imply scientific evidence for the development of functional foods using 11 plants from Tunisia which has oxidative stress and lipid accumulation reduction effects.

참고문헌 (46)

  1. Abdallah FB, "Caffeic acid and quercetin protect erythrocytes against the oxidative stress and the genotoxic effects of lambda-cyhalothrin in vitro", Hum Exp Toxicol, 31, pp.92~100 (2012)
  2. Afonso AF, "Metabolites and biological activities of Thymus zygis, Thymus pulegioides, and Thymus fragrantissimus grown under organic cultivation", Molecules, 23, pp.1514~ (2018)
  3. 안은미, "한국인 다소비 채소의 에탄올 추출물이 3T3-L1 지방세포와 HepG2 간장세포의 지질축적, MCP-1과 Adiponectin의 분비에 미치는 영향", 동아시아식생활학회지, 25(1), pp.99~110 (2015), 동아시아식생활학회[DOI]
  4. Alamri ZZ, "The role of liver in metabolism : An updated review with physiological emphasis", Int J Basic Clin Pharmacol, 7, pp.2271~2276 (2018)
  5. Amensour M, "Total phenolic content and antioxidant activity of myrtle(Myrtus communis)extracts", Nat Prod Commun, 4, pp.819~824 (2009)
  6. Balachander GJ, "Rosmarinic acid attenuates hepatic steatosis by modulating ER stress and autophagy in oleic acid-induced HepG2 cells", RSC Adv, 8, pp.26656~26663 (2018)
  7. Bayle GK, "Ecological and social impacts of eucalyptus tree plantation on the environment", J Biodivers Conserv Bioresour Manag, 5, pp.93~104 (2019)
  8. Bianchin M, "Antioxidant properties of lyophilized rosemary and sage extracts and its effect to prevent lipid oxidation in poultry pate", Molecules, 25, pp.5160~ (2020)
  9. Brieger K, "Reactive oxygen species: From health to disease", Swiss Med Wkly, 142, pp.w13659~ (2012)
  10. Cory H, "The role of polyphenols in human health and food systems : A mini-review", Front Nutr, 5, pp.87~ (2018)
  11. Stefano Dall'Acqua, "Phytochemical Composition and Antioxidant Activity of Laurus nobilis L. Leaf Infusion", Journal of Medicinal Food, 12(4), pp.869~876 (2009), 한국식품영양과학회
  12. Franco AM, "Myrtle seeds (Myrtus communis L.) as a rich source of the bioactive ellagitannins oenothein B and eugeniflorin D2", ACS Omega, 4, pp.15966~15974 (2019)
  13. Gamoun M, "Diversity of desert rangelands of Tunisia", Plant Divers, 40, pp.217~225 (2018)
  14. Guan YS, "Plants consumption and liver health", Evid-Based Complementary Altern Med, 2015, pp.824185~ (2015)
  15. Gurnani N, "Chemical composition, total phenolic and flavonoid contents, and in vitro antimicrobial and antioxidant activities of crude extracts from red chilli seeds (Capsicum frutescens L.)", J Taibah Univ Sci, 10, pp.462~470 (2016)
  16. Hassine DB, "Chemical composition and in vitro evaluation of the antioxidant and antimicrobial activities of Eucalyptus gillii essential oil and extracts", Molecules, 17, pp.9540~9558 (2012)
  17. Hu Y, "Protective efficacy of carnosic acid against hydrogen peroxide induced oxidative injury in HepG2 cells through the SIRT1 pathway", Can J Physiol Pharmacol, 93, pp.625~631 (2015)
  18. Iauk L, "Antibacterial, antioxidant and hypoglycaemic effects of Thymus capitatus (L.) Hoffmanns. et Link leaves’ fractions", J Enzyme Inhib Med Chem, 30, pp.360~365 (2015)
  19. Kawano Y, "Mechanisms of hepatic triglyceride accumulation in non-alcoholic fatty liver disease", J Gastroenterology, 48, pp.431~441 (2013)
  20. Kindl M, "Antioxidant and anticholinesterase potential of six Thymus species", Evid-Based Complementary Altern Med, 2015, pp.403950~ (2015)
  21. Kumar P, "Effect of quercetin on lipid peroxidation and changes in lung morphology in experimental influenza virus infection", Int J Exp Path, 84, pp.127~133 (2003)
  22. Lam PL, "The role of reactive oxygen species in the biological activity of antimicrobial agents : An updated mini review", Chem Biol Interact, 320, pp.109023~ (2020)
  23. Lewoyehu M, "Comparative evaluation of analytical methods for determining the antioxidant activities of honey : A review", Cog Food Agric, 5, pp.1685059~ (2019)
  24. Li R, "Defining ROS in biology and medicine", React Oxyg Species(Apex), 1, pp.9~21 (2016)
  25. Mahmoud MAOM, "Genetic diversity in local barley accessions collected from different geographical regions of Tunisia", Plant Genet Resour, 7, pp.169~176 (2009)
  26. Milia E, "The pharmaceutical ability of Pistacia lentiscus L. leaves essential oil against periodontal bacteria and candida sp. and its anti-inflammatory potential", Antibiotics, 9, pp.281~ (2020)
  27. Morita M, "Fatty liver induced by free radicals and lipid peroxidation", Free Radic Res, 46, pp.758~765 (2012)
  28. Mulas M, "Cultivar selection from rosemary (Rosmarinus officinalis L.) spontaneous populations in the mediterranean area", Acta Hortic, 676, pp.127~133 (2005)
  29. Niki E, "Lipid peroxidation : Mechanisms, inhibition, and biological effects", Biochem Biophys Res Commun, 338, pp.668~676 (2005)
  30. Panche AN, "Flavonoids: An overview", J Nutr Sci, 5, pp.e47~ (2016)
  31. Park JS, "The factors related to the incidence of non-alcoholic-fatty-liver-disease(NAFLD)in obese patients", Soonchunhyang Med Sci, 15, pp.75~82 (2009)
  32. Remila S, "Antioxidant, cytoprotective, anti-inflammatory and anticancer activities of Pistacia lentiscus(Anacardiaceae)leaf and fruit extracts", Eur J Integr Med, 7, pp.274~286 (2015)
  33. Rodriguez-Echeverria S, "Genetic diversity and differentiation of Ammophila arenaria (L.) link as revealed by ISSR markers", J Coastal Res, 24, pp.122~126 (2008)
  34. Romani A, "Identification and quantitation of polyphenols in leaves of Myrtus communis L", Chromatographia, 49, pp.17~20 (1999)
  35. Sayah K, "Antioxidant activity and inhibitory potential of Cistus salviifolius (L.) and Cistus monspeliensis (L.) aerial parts extracts against key enzymes linked to hyperglycemia", BioMed Res Int, 2017, pp.2789482~ (2017)
  36. Shin SK, "Catalase and nonalcoholic fatty liver disease", Pflugers Arch-Eur J Physiol, 470, pp.1721~1737 (2018)
  37. Stefan PN, "Non-alcoholic fatty liver disease : Causes, diagnosis, cardiometabolic consequences, and treatment strategies", Lancet Diabetes Endocrinol, 7, pp.313~324 (2019)
  38. Stratil P, "Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods", J Agric Food Chem, 54, pp.607~616 (2006)
  39. Tabana YM, "In vitro anti-metastatic and antioxidant activity of Nicotiana glauca fraction against breast cancer cells", Adv Biol Res, 9, pp.95~102 (2015)
  40. Tungmunnithum D, "Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects : An overview", Medicines(Basel), 5, pp.93~ (2018)
  41. Vergara D, "Oxidative molecular mechanisms underlying liver diseases : From systems biology to the personalized medicine", Oxid Med Cell Longevity, 2019, pp.7864316~ (2019)
  42. Vuppalanchi R, "Oxidative stress in chronic liver disease : Relationship between peripheral and hepatic measurements", Am J Med Sci, 342, pp.314~317 (2011)
  43. Wahyono A, "Antioxidant activity and total phenolic contents of bread enriched with pumpkin flour", IOP Conf Ser Earth Environ Sci, 411, pp.012049~ (2020)
  44. Walker JM, "The bicinchoninic acid(BCA)assay for protein quantitation", Methods Mol Biol, 32, pp.5~8 (1994)
  45. 유하나, "Plant diversity in different bioclimatic zones in Tunisia", Journal of Asia-Pacific Biodiversity, 9(1), pp.56~62 (2016), 국립중앙과학관
  46. 윤영, "오디 당침출액의 HepG2 세포에서 H2O2로 야기된 산화적 스트레스 보호 효과", 한국식품저장유통학회지, 22(5), pp.751~757 (2015), 한국식품저장유통학회

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