Listeria monocytogenes의 검출을 위한 loop-mediated isothermal amplification, real-time PCR, 배지 도말법의 검출 성능 및 특성 비교
Comparative efficacy of loop-mediated isothermal amplification, real-time PCR, and selective agar method for detection of Listeria monocytogenes in food
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Listeria monocytogenes is a foodborne pathogen causing listeriosis, which can be fatal in specific high-risk groups. The aim of this study was to compare the performance (accuracy, sensitivity, and specificity) of 3MTM Molecular Detection System (3MTM MDS) and Korean Food Codex [real-time PCR (RT-PCR) and selective agar] for the detection of L. monocytogenes in various food matrices. The detection performance of the three methods was determined against 100-103 CFU/mL of L. monocytogenes in vitro and showed high accuracy in the order of RT-PCR, 3MTM MDS, and selective agar. There was no difference in sensitivity and specificity of the three methods. Eleven food matrices, selected from agricultural, livestock, and seafood products, were artificially inoculated with 100-103 CFU/25 g of L. monocytogenes and enriched in 3MTM Demi-Fraser Broth. None of the three methods could completely detect low concentrations of L. monocytogenes in a food matrix. However, 3MTM MDS, which is a loop-mediated isothermal amplification (LAMP)-based technology for rapid detection, showed a higher positive detection rate than RT-PCR did, but lower than that of selective agar. These data indicated that 3MTM MDS was superior for the rapid detection of L. monocytogenes, compared to RT-PCR in food matrices containing various inhibitors. Consequentially, the study findings suggest that the LAMP method is a promising alternative to RT-PCR for the rapid detection of foodborne pathogens.
Abstract
Listeria monocytogenes is a foodborne pathogen causing listeriosis, which can be fatal in specific high-risk groups. The aim of this study was to compare the performance (accuracy, sensitivity, and specificity) of 3MTM Molecular Detection System (3MTM MDS) and Korean Food Codex [real-time PCR (RT-PCR) and selective agar] for the detection of L. monocytogenes in various food matrices. The detection performance of the three methods was determined against 100-103 CFU/mL of L. monocytogenes in vitro and showed high accuracy in the order of RT-PCR, 3MTM MDS, and selective agar. There was no difference in sensitivity and specificity of the three methods. Eleven food matrices, selected from agricultural, livestock, and seafood products, were artificially inoculated with 100-103 CFU/25 g of L. monocytogenes and enriched in 3MTM Demi-Fraser Broth. None of the three methods could completely detect low concentrations of L. monocytogenes in a food matrix. However, 3MTM MDS, which is a loop-mediated isothermal amplification (LAMP)-based technology for rapid detection, showed a higher positive detection rate than RT-PCR did, but lower than that of selective agar. These data indicated that 3MTM MDS was superior for the rapid detection of L. monocytogenes, compared to RT-PCR in food matrices containing various inhibitors. Consequentially, the study findings suggest that the LAMP method is a promising alternative to RT-PCR for the rapid detection of foodborne pathogens.
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