Biofilm formation on denture base resin including ZnO, CaO, and TiO2 nanoparticles
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
PURPOSE. This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and TiO2 on biofilm formation. MATERIALS AND METHODS. Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and TiO2 nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens. Surface roughness was determined by employing a profilometric approach; biofilm formation was simulated using a monospecies Candida albicans biofilm model and a multispecies biofilm model including C. albicans, Actinomyces naeslundii, and Streptococcus gordonii. Relative viable biomass was determined after 20 hours and 44 hours using a MTT-based approach. RESULTS. No statistically significant disparities were identified among the various materials regarding surface roughness and relative viable biomass. CONCLUSION. The results indicate that doping denture base resin materials with commercially available ZnO, CaO, or TiO2 nanopowders do not inhibit biofilm formation on their surface. Further studies might address the impact of varying particle sizes as well as increasing the fraction of nanoparticles mixed into the acrylic resin matrix.
Abstract
PURPOSE. This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and TiO2 on biofilm formation. MATERIALS AND METHODS. Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and TiO2 nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens. Surface roughness was determined by employing a profilometric approach; biofilm formation was simulated using a monospecies Candida albicans biofilm model and a multispecies biofilm model including C. albicans, Actinomyces naeslundii, and Streptococcus gordonii. Relative viable biomass was determined after 20 hours and 44 hours using a MTT-based approach. RESULTS. No statistically significant disparities were identified among the various materials regarding surface roughness and relative viable biomass. CONCLUSION. The results indicate that doping denture base resin materials with commercially available ZnO, CaO, or TiO2 nanopowders do not inhibit biofilm formation on their surface. Further studies might address the impact of varying particle sizes as well as increasing the fraction of nanoparticles mixed into the acrylic resin matrix.
참고문헌 (11)
- Nitschke I, "Funfte Deutsche Mundgesundheitsstudie", pp.416~451 (2016), Deutscher Zahnarzte Verlag DAV
- Gendreau L, "Epidemiology and etiology of denture stomatitis", J Prosthodont, 20, pp.251~260 (2011)
- Budtz-Jorgensen E, "Denture stomatitis. I. The etiology in relation to trauma and infection", Acta Odontol Scand, 28, pp.71~92 (1970)
- Sawai J, "Evaluation of growth inhibitory effect of ceramics powder slurry on bacteria by conductance method", J Chem Eng Jpn, 28, pp.288~293 (1995)
- Sawai J, "Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay", J Appl Microbiol, 96, pp.803~809 (2004)
- Arai T, "Inhibiting microbial adhesion to denture base acrylic resin by titanium dioxide coating", J Oral Rehabil, 36, pp.902~908 (2009)
- Azam A, "Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study", Int J Nanomedicine, 7, pp.6003~6009 (2012)
- Raghupathi KR, "Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles", Langmuir, 27, pp.4020~4028 (2011)
- Susewind S, "Biofilm formation and Candida albicans morphology on the surface of denture base materials", Mycoses, 58, pp.719~727 (2015)
- Cierech M, "Significance of polymethylmethacrylate (PMMA) modification by zinc oxide nanoparticles for fungal biofilm formation", Int J Pharm, 510, pp.323~335 (2016)
- Dizaj SM, "Antimicrobial activity of the metals and metal oxide nanoparticles", Mater Sci Eng C Mater Biol Appl, 44, pp.278~284 (2014)