Partial denture metal framework may harbor potentially pathogenic bacteria
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
PURPOSE. The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time. MATERIALS AND METHODS. This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity). RESULTS. A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2. CONCLUSION. The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.
Abstract
PURPOSE. The aim of this study was to characterize and compare bacterial diversity on the removable partial denture (RPD) framework over time. MATERIALS AND METHODS. This descriptive pilot study included five women who were rehabilitated with free-end mandibular RPD. The biofilm on T-bar clasps were collected 1 week (t1) and 4 months (t2) after the RPD was inserted (t0). Bacterial 16S rDNA was extracted and PCR amplified. Amplicons were cloned; clones were submitted to cycle sequencing, and sequences were compared with GenBank (98% similarity). RESULTS. A total of 180 sequences with more than 499 bp were obtained. Two phylogenetic trees with 84 (t1) and 96 (t2) clones represented the bacteria biofilm at the RPD. About 93% of the obtained phylotypes fell into 25 known species for t1 and 17 for t2, which were grouped in 5 phyla: Firmicutes (t1=82%; t2=60%), Actinobacteria (t1=5%; t2=10%), Bacteroidetes (t1=2%; t2=6%), Proteobacteria (t1=10%; t2=15%) and Fusobacteria (t1=1%; t2=8%). The libraries also include 3 novel phylotypes for t1 and 11 for t2. Library t2 differs from t1 (P=.004); t1 is a subset of the t2 (P=.052). Periodontal pathogens, such as F. nucleatum, were more prevalent in t2. CONCLUSION. The biofilm composition of the RPD metal clasps changed along time after RPD wearing. The RPD framework may act as a reservoir for potentially pathogenic bacteria and the RPD wearers may benefit from regular follow-up visits and strategies on prosthesis-related oral health instructions.
참고문헌 (38)
- Bergman B, "A 25 year longitudinal study of patients treated with removable partial dentures", J Oral Rehabil, 22, pp.595~599 (1995)
- Mullally BH, "Periodontal status of regular dental attenders with and without removable partial dentures", Eur J Prosthodont Restor Dent, 2, pp.161~163 (1994)
- Schwalm CA, "A clinical study of patients 1 to 2 years after placement of removable partial dentures", J Prosthet Dent, 38, pp.380~391 (1977)
- Zlatarić DK, "The effect of removable partial dentures on periodontal health of abutment and non-abutment teeth", J Periodontol, 73, pp.137~144 (2002)
- do Amaral BA, "A clinical followup study of the periodontal conditions of RPD abutment and non-abutment teeth", J Oral Rehabil, 37, pp.545~552 (2010)
- Vanzeveren C, "Influence of removable partial denture on periodontal indices and microbiological status", J Oral Rehabil, 29, pp.232~239 (2002)
- Zhu X, "Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE", World J Microbiol Biotechnol, 28, pp.2229~2236 (2012)
- Campos MS, "Biofilm microbial communities of denture stomatitis", Oral Microbiol Immunol, 23, pp.419~424 (2008)
- Danser MM, "Putative periodontal pathogens colonizing oral mucous membranes in denture-wearing subjects with a past history of periodontitis", J Clin Periodontol, 22, pp.854~859 (1995)
- Diaz PI, "Molecular characterization of subject-specific oral microflora during initial colonization of enamel", Appl Environ Microbiol, 72, pp.2837~2848 (2006)
- Fernandes CB, "Do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens?", Tex Dent J, 129, pp.751~761 (2012)
- Hutter G, "Molecular analysis of bacteria in periodontitis:evaluation of clone libraries, novel phylotypes and putative pathogens", Microbiology, 149, pp.67~75 (2003)
- Ledder RG, "Molecular analysis of the subgingival microbiota in health and disease", Appl Environ Microbiol, 73, pp.516~523 (2007)
- Mantzourani M, "Nonoral bifidobacteria and the aciduric microbiota of the denture plaque biofilm", Mol Oral Microbiol, 25, pp.190~199 (2010)
- Marchini L, "Bacterial diversity in aphthous ulcers", Oral Microbiol Immunol, 22, pp.225~231 (2007)
- Paster BJ, "Bacterial diversity in human subgingival plaque", J Bacteriol, 183, pp.3770~3783 (2001)
- Sachdeo A, "Biofilms in the edentulous oral cavity", J Prosthodont, 17, pp.348~356 (2008)
- Wade WG, "Molecular detection of novel anaerobic species in dentoalveolar abscesses", Clin Infect Dis, 25, pp.S235~S236 (1997)
- Mihalow DM, "The influence of removable partial dentures on the level of Streptococcus mutans in saliva", J Prosthet Dent, 59, pp.49~51 (1988)
- Rocha EP, "Longitudinal study of the influence of removable partial denture and chemical control on the levels of Streptococcus mutans in saliva", J Oral Rehabil, 30, pp.131~138 (2003)
- Yasui M, "Colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers", Gerodontology, 29, pp.e494~e502 (2012)
- Grivet M, "Effect of hydrophobicity on in vitro streptococcal adhesion to dental alloys", J Mater Sci Mater Med, 11, pp.637~642 (2000)
- Mine K, "Microbiological risk for periodontitis of abutment teeth in patients with removable partial dentures", J Oral Rehabil, 36, pp.696~702 (2009)
- Aas JA, "Defining the normal bacterial flora of the oral cavity", J Clin Microbiol, 43, pp.5721~5732 (2005)
- Dewhirst FE, "The human oral microbiome", J Bacteriol, 192, pp.5002~5017 (2010)
- Urushibara Y, "An analysis of the biofilms adhered to framework alloys using in vitro denture plaque models", Dent Mater J, 33, pp.402~414 (2014)
- Carr AB, "McCracken’s Removable Partial Prosthodontics" (2010), Mosby
- Chao A, "Non-parametric estimation of the number of classes in a population", Scand J Stat, 11, pp.265~270 (1984)
- Chao A, "Estimating the number of classes via sample coverage", J Am Stat Assoc, 87, pp.210~217 (1992)
- Marsh PD, "The influence of denture-wearing and age on the oral microflora", J Dent Res, 71, pp.1374~1381 (1992)
- Nyvad B, "Microbiology of the early colonization of human enamel and root surfaces in vivo", Scand J Dent Res, 95, pp.369~380 (1987)
- Taylor R, "Retention of oral microorganisms on cobalt-chromium alloy and dental acrylic resin with different surface finishes", J Prosthet Dent, 80, pp.592~597 (1998)
- Socransky SS, "Microbial complexes in subgingival plaque", J Clin Periodontol, 25, pp.134~144 (1998)
- Siqueira JF Jr, "Catonella morbi and Granulicatella adiacens: new species in endodontic infections", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102, pp.259~264 (2006)
- Gonçalves LF, "Levels of Selenomonas species in generalized aggressive periodontitis", J Periodontal Res, 47, pp.711~718 (2012)
- Cone LA, "Actinomyces odontolyticus bacteremia", Emerg Infect Dis, 9, pp.1629~1632 (2003)
- Haffajee AD, "Factors affecting human supragingival biofilm composition. I. Plaque mass", J Periodontal Res, 44, pp.511~519 (2009)
- Rüdiger SG, "Dental biofilms at healthy and inflamed gingival margins", J Clin Periodontol, 29, pp.524~530 (2002)