Effects of Cold Agglutinin on the Accuracy of Complete Blood Count Results and Optimal Sample Pretreatment Protocols for Eliminating Such Effects
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Dear Editor, Cold agglutinin is an autoantibody that causes autoimmune hemolytic anemia by binding to I/i carbohydrate antigens on the red blood cell (RBC) surface [1]. While RBC agglutination causes clinical symptoms of hemolytic anemia, agglutination caused by cold agglutinin is a notorious pre-analytical and analytical factor that leads to spurious automated complete blood count (CBC) results [2]. Although RBC agglutination falsely increases mean corpuscular volume (MCV), effects of cold agglutinin on other CBC parameters have not been widely studied [3, 4]. The effects of cold agglutinin on CBC results have been evaluated mainly for a limited number of parameters, on old versions of automated CBC analyzers [5, 6], which have undergone remarkable technical improvements in the last decade. We present a case of chronic cold agglutinin disease, and report the effects of cold agglutinin on CBC parameters based on whole blood samples comparatively analyzed by four automated CBC analyzers commonly used in current clinical laboratories.
Abstract
Dear Editor, Cold agglutinin is an autoantibody that causes autoimmune hemolytic anemia by binding to I/i carbohydrate antigens on the red blood cell (RBC) surface [1]. While RBC agglutination causes clinical symptoms of hemolytic anemia, agglutination caused by cold agglutinin is a notorious pre-analytical and analytical factor that leads to spurious automated complete blood count (CBC) results [2]. Although RBC agglutination falsely increases mean corpuscular volume (MCV), effects of cold agglutinin on other CBC parameters have not been widely studied [3, 4]. The effects of cold agglutinin on CBC results have been evaluated mainly for a limited number of parameters, on old versions of automated CBC analyzers [5, 6], which have undergone remarkable technical improvements in the last decade. We present a case of chronic cold agglutinin disease, and report the effects of cold agglutinin on CBC parameters based on whole blood samples comparatively analyzed by four automated CBC analyzers commonly used in current clinical laboratories.
참고문헌 (10)
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