Genetic Diversity of Toxoplasma gondii Strains from Different Hosts and Geographical Regions by Sequence Analysis of GRA20 Gene
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Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.
Abstract
Toxoplasma gondii is a eukaryotic parasite of the phylum Apicomplexa, which infects all warm-blood animals, including humans. In the present study, we examined sequence variation in dense granule 20 (GRA20) genes among T. gondii isolates collected from different hosts and geographical regions worldwide. The complete GRA20 genes were amplified from 16 T. gondii isolates using PCR, sequence were analyzed, and phylogenetic reconstruction was analyzed by maximum parsimony (MP) and maximum likelihood (ML) methods. The results showed that the complete GRA20 gene sequence was 1,586 bp in length among all the isolates used in this study, and the sequence variations in nucleotides were 0-7.9% among all strains. However, removing the type III strains (CTG, VEG), the sequence variations became very low, only 0-0.7%. These results indicated that the GRA20 sequence in type III was more divergence. Phylogenetic analysis of GRA20 sequences using MP and ML methods can differentiate 2 major clonal lineage types (type I and type III) into their respective clusters, indicating the GRA20 gene may represent a novel genetic marker for intraspecific phylogenetic analyses of T. gondii.
참고문헌 (20)
- Montoya JG, "Toxoplasmosis", Lancet, 363, pp.1965~1976 (2004)
- Weiss LM, "Toxoplasmosis: a history of clinical observations", Int J Parasitol, 39, pp.895~901 (2009)
- Dubey JP, "Toxoplasmosis of animals and humans" (2010), CRC Press
- Howe DK, "Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease", J Infect Dis, 172, pp.1561~1566 (1995)
- Melo MB, "Toxoplasma gondii effectors are master regulators of the inflammatory response", Trends Parasitol, 27, pp.487~495 (2011)
- Hunter CA, "Modulation of innate immunity by Toxoplasma gondii virulence effectors", Nat Rev Microbiol, 10, pp.766~778 (2012)
- Hsiao CHC, "A HT", PEXEL motif in Toxoplasma dense granule proteins is a signal for protein cleavage but not export into the host cell, 2013, pp.14, 519~531
- Rosowski EE, "Strain-specific activation of the NF-kB pathway by GRA15, a novel Toxoplasma gondii dense granule protein", J Exp Med, 208, pp.195~212 (2011)
- Chen J, "Genetic diversity among Toxoplasma gondii strains from different hosts and geographical regions revealed by sequence analysis of GRA5 gene", Parasit Vectors, 5, pp.279~ (2012)
- Fazaeli A, "Molecular typing of Toxoplasma gondii strains by GRA6 gene sequence analysis", Int J Parasitol, 30, pp.637~642 (2000)
- Zhou P, "Genetic characterization of Toxoplasma gondii isolates from China", Parasitol Int, 58, pp.193~195 (2009)
- Zhou P, "Genetic characterization of Toxoplasma gondii isolates from pigs in China", J Parasitol, 96, pp.1027~1029 (2010)
- Su C, "Moving towards an integrated approach to molecular detection and identification of Toxoplasma gondii", Parasitology, 137, pp.1~11 (2010)
- Thompson JD, "The CLUSTAL_X windows interface: fexible strategies for multiple sequence alignment aided by quality analysis tools", Nucleic Acids Res, 25, pp.4876~4882 (1997)
- Sousa S, "Selection of polymorphic peptides from GRA6 and GRA7 sequences of Toxoplasma gondii strains to be used in serotyping", Clin Vaccine Immunol, 16, pp.1158~1169 (2009)
- Ma JS, "Selective and strain-specifc NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6", J Exp Med, 211, pp.2013~2032 (2014)
- Zhang NZ, "Sequence variation in Toxoplasma gondii rop17 gene among strains from different hosts and geographical locations", Scientific World Journal, 2014, pp.349325~ (2014)
- Lu J, "Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations", Genet Mol Res, 13, pp.6906~6911 (2014)
- Xu Y, "Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations", Genet Mol Res, 13, pp.4839~4844 (2014)
- Chen J, "Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations", Genet Mol Res, 13, pp.3356~3361 (2014)