C3H/He Mice as an Incompatible Cholangiocarcinoma Model by Clonorchis sinensis, Dicyclanil and N-Nitrosodimethylamine
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Clonorchis sinensis is a Group-I bio-carcinogen, associated with cholangiocarcinoma (CCA). The hamster is the only experimental model of C. sinensis-mediated CCA, but we oblige another animal model. The present study intended to develop a C. sinensis (Cs) mediated CCA model using C3H/He mice, co-stimulated with N-nitrosodimethylamine (NDMA) and dicyclanil (DC). The mice were divided into 8 groups with different combinations of Cs, NDMA, and DC. Six months later the mice were sacrificed and subjected to gross and histopathological examination. The body weights were significantly reduced among the groups treated with 2 or more agents (eg. Cs+NDMA, Cs+DC, NDMA+DC, and Cs+NDMA+DC). In contrast, liver weight percentages to body weight were increased in above groups by 4.1% to 4.7%. A Change of the spleen weight was observed only in Cs+NDMA group. Though C. sinensis infection is evident from hyperplastic changes, only 1 worm was recovered. Two mice, 1 from Cs and the other from Cs+DC group, showed mass forming lesions; 1 (281.2 mm3) from the Cs group was a hepatocellular adenoma and the other (280.6 mm3) from the Cs+DC group was a cystic mass (peliosis). Higher prevalence of gray-white nodules was observed in Cs group (42.9%) followed by Cs+NDMA+DC group (21.4%). The mice of the Cs+NDMA+DC group showed hyper-proliferation of the bile duct with fibrotic changes. No characteristic change for CCA was recognized in any of the groups. In conclusion, C3H/He mice produce no CCA but extensive fibrosis when they are challenged by Cs, NDMA, and DC together.
Abstract
Clonorchis sinensis is a Group-I bio-carcinogen, associated with cholangiocarcinoma (CCA). The hamster is the only experimental model of C. sinensis-mediated CCA, but we oblige another animal model. The present study intended to develop a C. sinensis (Cs) mediated CCA model using C3H/He mice, co-stimulated with N-nitrosodimethylamine (NDMA) and dicyclanil (DC). The mice were divided into 8 groups with different combinations of Cs, NDMA, and DC. Six months later the mice were sacrificed and subjected to gross and histopathological examination. The body weights were significantly reduced among the groups treated with 2 or more agents (eg. Cs+NDMA, Cs+DC, NDMA+DC, and Cs+NDMA+DC). In contrast, liver weight percentages to body weight were increased in above groups by 4.1% to 4.7%. A Change of the spleen weight was observed only in Cs+NDMA group. Though C. sinensis infection is evident from hyperplastic changes, only 1 worm was recovered. Two mice, 1 from Cs and the other from Cs+DC group, showed mass forming lesions; 1 (281.2 mm3) from the Cs group was a hepatocellular adenoma and the other (280.6 mm3) from the Cs+DC group was a cystic mass (peliosis). Higher prevalence of gray-white nodules was observed in Cs group (42.9%) followed by Cs+NDMA+DC group (21.4%). The mice of the Cs+NDMA+DC group showed hyper-proliferation of the bile duct with fibrotic changes. No characteristic change for CCA was recognized in any of the groups. In conclusion, C3H/He mice produce no CCA but extensive fibrosis when they are challenged by Cs, NDMA, and DC together.
참고문헌 (23)
- Bouvard V, "WHO International Agency for Research on Cancer Monograph Working Group. A review of human carcinogens-Part B: biological agents", Lancet Oncol, 10, pp.321~322 (2009)
- 신해림, "Descriptive Epidemiology of Cholangiocarcinoma and Clonorchiasis in Korea", Journal of Korean Medical Science, 25(7), pp.1011~1016 (2010), 대한의학회
- UDDINMD HAFIZ, "In Vitro Maintenance of Clonorchis sinensis Adult Worms", The Korean Journal of Parasitology, 50(4), pp.309~315 (2012), 대한기생충학ㆍ열대의학회
- Uddin MH, "Involvement of PSMD10, CDK4, and tumor suppressors in development of intrahepatic cholangiocarcinoma of syrian golden hamsters induced by Clonorchis sinensis and N-nitrosodimethylamine", PLoS Negl Trop Dis, 9, pp.e0004008~ (2015)
- Yongvanit P, "Risk biomarkers for assessment and chemoprevention of liver fluke-associated cholangiocarcinoma", J Hepatobiliary Pancreat Sci, 21, pp.309~315 (2014)
- Uddin MH, "Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice", Parasitol Int, 61, pp.118~123 (2012)
- Lee JH, "Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis", Korean J Parasitol, 32, pp.13~18 (1994)
- Ohnishi S, "DNA damage in inflammation-related carcinogenesis and cancer stem cells", Oxid Med Cell Longev, 2013, pp.387014~ (2013)
- Umemura T, "Detection of oxidative DNA damage, cell proliferation and in vivo mutagenicity induced by dicyclanil, a non-genotoxic carcinogen, using gpt delta mice", Mutat Res, 633, pp.46~54 (2007)
- Jensen MM, "Tumor volume in subcutaneous mouse xenografts measured by microCT is more accurate and reproducible than determined by 18F-FDG-microPET or external caliper", BMC Med Imaging, 8, pp.16~ (2008)
- Nourissat A, "Predictors of weight loss during radiotherapy in patients with stage I or II head and neck cancer", Cancer, 116, pp.2275~2283 (2010)
- Chen SP, "Evaluating probability of cancer among older people with unexplained, unintentional weight loss", Arch Gerontol Geriatr, 50(suppl 1), pp.S27~S29 (2010)
- Kaewpitoon N, "Opisthorchiasis in Thailand: review and current status", World J Gastroenterol, 14, pp.2297~2302 (2008)
- Lee JH, "Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamsters", Korean J Parasitol, 31, pp.21~30 (1993)
- Zeilmaker MJ, "Risk assessment of N-nitrosodimethylamine formed endogenously after fish-with-vegetable meals", Toxicol Sci, 116, pp.323~335 (2010)
- WHO, "N-nitrosodimethylamine in drinking-water: Background document for development of WHO Guidelines for drinking-water quality", World Health Organization, ~ (2006)
- Lindamood C 3rd, "Alkylation and de novo synthesis of liver cell DNA from C3H mice during continuous dimethylnitrosamine exposure", Cancer Res, 42, pp.4153~4157 (1982)
- Uehara T, "Susceptibility of liver proliferative lesions in heterozygous p53 deficient CBA mice to various carcinogens", J Vet Med Sci, 64, pp.551~556 (2002)
- Hu QW, "Effects of bicyclol on dimethylnitrosamine-induced liver fibrosis in mice and its mechanism of action", Life Sci, 79, pp.606~612 (2006)
- Moto M, "Absence of in vivo genotoxicity and liver initiation activity of dicyclanil", J Toxicol Sci, 28, pp.173~179 (2003)
- Moto M, "Gene expression analysis on the dicyclanil-induced hepatocellular tumors in mice", Toxicol Pathol, 34, pp.744~751 (2006)
- Zhou L, "Clonorchis sinensis lysophospholipase inhibits TGF-beta1-induced expression of pro-fibrogenic genes through attenuating the activations of Smad3, JNK2, and ERK1", 2 in hepatic stellate cell line LX-2, 2016, pp.115, 643~650
- Zheng M, "Characterization of a Clonorchis sinensis antigen, calmodulin, and its relationship with liver fibrosis", Nan Fang Yi Ke Da Xue Xue Bao, 35, pp.659~664 (2015)