Investigation of Dysregulation of Several MicroRNAs in Peripheral Blood of Schizophrenia Patients
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Objective: The prevalence of schizophrenia is 1%, and it is a debilitating disorder that often results in a shortened lifespan. Peripheral blood samples are good candidates to investigate because they can be easily drawn, and they are widely studied in psychiatric disorders. MicroRNAs are small non-coding RNA transcripts. They regulate the expression of genes by binding to the 3’-untranslated region (UTR) of mRNAs and pointing them to degrade. In this study, we aimed to investigate the expression of miR-9-5p, miR-29a-3p, miR-106-5p, miR-106b-5p, miR-107, miR-125a-3p, and miR-125b-3p in schizophrenia patients and healthy controls. Methods: We collected blood samples from 16 patients with schizophrenia and 16 healthy controls. MicroRNAs were measured with reverse transcriptase polymerase chain reaction. Results: Schizophrenia patients showed statistically significant upregulation of five microRNAs: miR9-5p (p=0.002), miR29a-3p (p＜0.001), miR106b-5p (p=0.002), miR125a-3p (p＜0.001), and miR125b-3p (p=0.018). Conclusion: Our results increased the value of the miR106 and miR29 families as potentially and consistently dysregulated in psychiatric disorders. Our results should be considered preliminary, and they need confirmation in future studies with larger sample sizes.
Abstract
Objective: The prevalence of schizophrenia is 1%, and it is a debilitating disorder that often results in a shortened lifespan. Peripheral blood samples are good candidates to investigate because they can be easily drawn, and they are widely studied in psychiatric disorders. MicroRNAs are small non-coding RNA transcripts. They regulate the expression of genes by binding to the 3’-untranslated region (UTR) of mRNAs and pointing them to degrade. In this study, we aimed to investigate the expression of miR-9-5p, miR-29a-3p, miR-106-5p, miR-106b-5p, miR-107, miR-125a-3p, and miR-125b-3p in schizophrenia patients and healthy controls. Methods: We collected blood samples from 16 patients with schizophrenia and 16 healthy controls. MicroRNAs were measured with reverse transcriptase polymerase chain reaction. Results: Schizophrenia patients showed statistically significant upregulation of five microRNAs: miR9-5p (p=0.002), miR29a-3p (p＜0.001), miR106b-5p (p=0.002), miR125a-3p (p＜0.001), and miR125b-3p (p=0.018). Conclusion: Our results increased the value of the miR106 and miR29 families as potentially and consistently dysregulated in psychiatric disorders. Our results should be considered preliminary, and they need confirmation in future studies with larger sample sizes.
참고문헌 (30)
- Anupa A. Vijayakumari, "Effect of Polymorphisms of Three Genes Mediating Monoamine Signalling on Brain Morphometry in Schizophrenia and Healthy Subjects", Clinical Psychopharmacology and Neuroscience, 13(1), pp.68~82 (2015), 대한정신약물학회
- Carissa Nadia Kuswanto, "Diffusion Tensor Imaging Findings of White Matter Changes in First Episode Schizophrenia: A Systematic Review", Clinical Psychopharmacology and Neuroscience, 10(1), pp.13~24 (2012), 대한정신약물학회[DOI]
- Annya M. Smyth, "The Neuroimmunology of Schizophrenia", Clinical Psychopharmacology and Neuroscience, 11(3), pp.107~117 (2013), 대한정신약물학회
- Biomarkers Definitions Working Group, "Biomarkers and surrogate endpoints: preferred definitions and conceptual framework", Clin Pharmacol Ther, 69, pp.89~95 (2001)
- Camkurt MA, "Comparison of plasma MicroRNA levels in drug naive, first episode depressed patients and healthy controls", J Psychiatr Res, 69, pp.67~71 (2015)
- Md. Mamun Al-Amin, "Effects of Antipsychotics on the Inflammatory Response System of Patients with Schizophrenia in Peripheral Blood Mononuclear Cell Cultures", Clinical Psychopharmacology and Neuroscience, 11(3), pp.144~151 (2013), 대한정신약물학회
- Mi-Hee Park, "Association between Intracellular Infectious Agents and Schizophrenia", Clinical Psychopharmacology and Neuroscience, 10(2), pp.117~123 (2012), 대한정신약물학회[DOI]
- Sasi Neelamekam, "Regulation of Interleukin-6 and Leptin in Schizophrenia Patients: A Preliminary Analysis", Clinical Psychopharmacology and Neuroscience, 12(3), pp.209~214 (2014), 대한정신약물학회
- Herken H, "Evidence that the activities of erythrocyte free radical scavenging enzymes and the products of lipid peroxidation are increased in different forms of schizophrenia", Mol Psychiatry, 6, pp.66~73 (2001)
- Filipowicz W, "Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight?", Nat Rev Genet, 9, pp.102~114 (2008)
- Novina CD, "The RNAi revolution", Nature, 430, pp.161~164 (2004)
- Place RF, "MicroRNA-373 induces expression of genes with complementary promoter sequences", Proc Natl Acad Sci U S A, 105, pp.1608~1613 (2008)
- Zhang J, "Comparative profiling of genes and miRNAs expressed in the newborn, young adult, and aged human epididymides", Acta Biochim Biophys Sin (Shanghai), 42, pp.145~153 (2010)
- Perkins DO, "microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder", Genome Biol, 8, pp.R27~ (2007)
- Beveridge NJ, "Schizophrenia is associated with an increase in cortical microRNA biogenesis", Mol Psychiatry, 15, pp.1176~1189 (2010)
- Liu X, "Identification of suitable plasma-based reference genes for miRNAome analysis of major depressive disorder", J Affect Disord, 163, pp.133~139 (2014)
- Kandemir H, "Evaluation of several micro RNA (miRNA) levels in children and adolescents with attention deficit hyperactivity disorder", Neurosci Lett, 580, pp.158~162 (2014)
- Wang WX, "The expression of microRNA miR-107decreases early in Alzheimer's disease and may accelerate disease progression through regulation of beta-site amyloid precursor protein-cleaving enzyme 1", J Neurosci, 28, pp.1213~1223 (2008)
- Edbauer D, "Regulation of synaptic structure and function by FMRP-associated microRNAs miR-125b and miR-132", Neuron, 65, pp.373~384 (2010)
- Shi W, "Aberrant expression of serum miRNAs in schizophrenia", J Psychiatr Res, 46, pp.198~204 (2012)
- Song HT, "A preliminary analysis of association between the down-regulation of microRNA-181b expression and symptomatology improvement in schizophrenia patients before and after antipsychotic treatment", J Psychiatr Res, 54, pp.134~140 (2014)
- Wong N, "miRDB: an online resource for microRNA target prediction and functional annotations", Nucleic Acid Res, 43, pp.D146~D152 (2015)
- Emamian ES, "AKT", GSK3 signaling pathway and schizophrenia, 2012, pp.5, 33~
- Magri C, "Study on GRIA2, GRIA3 and GRIA4genes highlights a positive association between schizophrenia and GRIA3 in female patients", Am J Med Genet B Neuropsychiatr Genet, 147B, pp.745~753 (2008)
- Abdolmaleky HM, "Epigenetic dysregulation of HTR2A in the brain of patients with schizophrenia and bipolar disorder", Schizophr Res, 129, pp.183~190 (2011)
- Bowden NA, "Altered gene expression in the superior temporal gyrus in schizophrenia", BMC Genomics, 9, pp.199~ (2008)
- Olney JW, "Glutamate receptor dysfunction and schizophrenia", Arch Gen Psychiatry, 52, pp.998~1007 (1995)
- Kim JS, "Low cerebrospinal fluid glutamate in schizophrenic patients and a new hypothesis on schizophrenia", Neurosci Lett, 20, pp.379~382 (1980)
- Eastwood SL, "Decreased expression of vesicular glutamate transporter 1 and complexin II mRNAs in schizophrenia:further evidence for a synaptic pathology affecting glutamate neurons", Schizophr Res, 73, pp.159~172 (2005)
- Gardiner E, "Imprinted DLK1-DIO3 region of 14q32 defines a schizophrenia-associated miRNA signature in peripheral blood mononuclear cells", Mol Psychiatry, 17, pp.827~840 (2012)