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Dynamic Thiol/Disulfide Homeostasis and Oxidative DNA Damage in Adult Attention Deficit Hyperactivity Disorder

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Objective: Insufficient number of oxidative stress studies have been conducted in patients with adult attention deficit hyperactivity disorder (ADHD). The objective of the current study is to examine the thiol/disulfide homeostasis as well as oxidative DNA damage levels in adult ADHD patients and to compare them with the results of healthy control subjects. Methods: The study was inclusive of forty-nine patients who were diagnosed with adult ADHD, as well as thirty-three healthy volunteers to be used as the control group. The diagnosis of the patients was conducted according to the DSM-5 diagnostic criteria. Blood were stored under appropriate laboratory conditions. For the purpose of detecting the oxidative DNA damage level, an extraction of genomic DNA from leukocytes was carried out, and furthermore the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), apart from deoxyguanosine, were measured accordingly. Results: Total thiol and the native thiol levels were observed to be statistically lower in adult ADHD patients as compared to the subjects in the healthy control group (p = 0.001). It was observed that the disulfide levels were higher in adult ADHD patients as compared to the healthy control subjects (p = 0.001). In addition, the levels of 8-OHdG, which are considered as a marker for assessing DNA damage, were found to be significantly lower in the control group as compared to the adult ADHD patients (p = 0.001). Conclusion: It was observed that the thiol/disulfide homeostasis had shifted towards disulfide, and 8-OHdG levels were increased in adult ADHD patients.

Abstract

Objective: Insufficient number of oxidative stress studies have been conducted in patients with adult attention deficit hyperactivity disorder (ADHD). The objective of the current study is to examine the thiol/disulfide homeostasis as well as oxidative DNA damage levels in adult ADHD patients and to compare them with the results of healthy control subjects. Methods: The study was inclusive of forty-nine patients who were diagnosed with adult ADHD, as well as thirty-three healthy volunteers to be used as the control group. The diagnosis of the patients was conducted according to the DSM-5 diagnostic criteria. Blood were stored under appropriate laboratory conditions. For the purpose of detecting the oxidative DNA damage level, an extraction of genomic DNA from leukocytes was carried out, and furthermore the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), apart from deoxyguanosine, were measured accordingly. Results: Total thiol and the native thiol levels were observed to be statistically lower in adult ADHD patients as compared to the subjects in the healthy control group (p = 0.001). It was observed that the disulfide levels were higher in adult ADHD patients as compared to the healthy control subjects (p = 0.001). In addition, the levels of 8-OHdG, which are considered as a marker for assessing DNA damage, were found to be significantly lower in the control group as compared to the adult ADHD patients (p = 0.001). Conclusion: It was observed that the thiol/disulfide homeostasis had shifted towards disulfide, and 8-OHdG levels were increased in adult ADHD patients.

참고문헌 (49)

  1. American Psychiatric Association, "Diagnostic and statistical manual of mental disorders: DSM-5" (2013), American Psychiatric Pub
  2. Wolraich ML, "Clinical practice guideline for the diagnosis, evaluation, and treatment of attention-deficit", hyperactivity disorder in children and adolescents, 2019, pp.144, e20192528~
  3. Barkley RA, "The persistence of attention-deficit", hyperactivity disorder into young adulthood as a function of reporting source and definition of disorder, 2002, pp.111, 279~289
  4. Meza JI, "Stigma and attention-deficit", hyperactivity disorder : negative perceptions and anger emotional reactions mediate the link between active symptoms and social distance, 2019, pp.11, 373~382
  5. Smalley SL, "Familial clustering of symptoms and disruptive behaviors in multiplex families with attention-deficit", hyperactivity disorder, 2000, pp.39, 1135~1143
  6. Barkley RA, "Attention-deficit hyperactivity disorder: a handbook for diagnosis and treatment" (2014), Guilford Publications
  7. Nigg JT, "Variation in an iron metabolism gene moderates the associa-tion between blood lead levels and attention-deficit", hyperactivity disorder in children, 2016, pp.27, 257~269
  8. Nigg JT, "Prenatal smoking exposure, low birth weight, and disruptive behavior disorders", J Am Acad Child Adolesc Psychiatry, 46, pp.362~369 (2007)
  9. Knopik VS, "Contributions of parental alcoholism, prenatal substance exposure, and genetic transmission to child ADHD risk : a female twin study", Psychol Med, 35, pp.625~635 (2005)
  10. Del Campo N, "The roles of dopamine and noradrenaline in the pathophysiology and treatment of attention-deficit", hyperactivity disorder, 2011, pp.69, e145~
  11. Ceylan M, "Oxidative imbalance in child and adolescent patients with attention-deficit", hyperactivity disorder, 2010, pp.34, 1491~1494
  12. Kawatani M, "Evaluation of oxidative stress status in children with pervasive developmental disorder and attention deficit hyperactivity disorder using urinary-specific biomarkers", Redox Rep, 16, pp.45~46 (2011)
  13. Ceylan MF, "Changes in oxidative stress and cellular immunity serum markers in attention-deficit", hyperactivity disorder, 2012, pp.66, 220~226
  14. Altan N, "Diabetes mellitus and oxidative stress", Turk J Biochem, 31, pp.51~56 (2006)
  15. Maes M, "In depression, bacterial translocation may drive inflammatory responses, oxidative and nitrosative stress(O&NS), and autoimmune responses directed against O&NS-damaged neoepitopes", Acta Psychiatr Scand, 127, pp.344~354 (2013)
  16. Ng F, "Oxidative stress in psychiatric disorders : evidence base and therapeutic implications", Int J Neuropsychopharmacol, 11, pp.851~876 (2008)
  17. Circu ML, "Reactive oxygen species, cellular redox systems, and apoptosis", Free Radic Biol Med, 48, pp.749~762 (2010)
  18. Milenkovic D, "miRNA as molecular target of polyphenols underlying their biological effects", Free Radic Biol Med, 64, pp.40~51 (2013)
  19. Topcuoglu C, "Thiol", disulfide homeostasis in untreated schizophrenia patients, 2017, pp.251, 212~216
  20. Eren MA, "The evaluation of thiol", disulphide homeostasis in diabetic nephropathy, 2019, pp.148, 249~253
  21. Kayalı R, "Protein oksidasyonunun ana mekanizmalari", Cerrahpasa Med J, 35, pp.83~89 (2004)
  22. Biswas S, "Redox modifications of protein-thiols : emerging roles in cell signaling", Biochem Pharmacol, 71, pp.551~564 (2006)
  23. Valko M, "Free radicals, metals and antioxidants in oxidative stress-induced cancer", Chem Biol Interact, 160, pp.1~40 (2006)
  24. Long JD, "8OHdG as a marker for Huntington disease progression", Neurobiol Dis, 46, pp.625~634 (2012)
  25. Yokuş B, "Biomarker of invivo oxidative DNA damage; 8-hydroxy-2'-deoxyguanosine", Turkiye Klinikleri Tıp Bilimleri Dergisi, 22, pp.535~543 (2002)
  26. Alpak G, "High catalase and low thiol levels in adult-ADHD patients", Bull Clin Psychopharmacol, 24, pp.128~134 (2014)
  27. Oztop D, "Oxidative stress in children with attention deficit hyperactivity disorder", Clin Biochem, 45, pp.745~748 (2012)
  28. Erzin G, "Thiol", disulphide homeostasis in bipolar disorder, 2018, pp.261, 237~242
  29. Kaur H, "Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection", Biochem J, 318(Pt 1), pp.21~23 (1996)
  30. Shigenaga MK, "Assays of oxidative DNA damage biomarkers 8-oxo-2'-deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluids by high-performance liquid chromatography with electrochemical detection", Methods Enzymol, 234, pp.16~33 (1994)
  31. Chovanová Z, "Effect of polyphenolic extract, Pycnogenol, on the level of 8-oxoguanine in children suffering from attention deficit", hyperactivity disorder, 2006, pp.40, 1003~1010
  32. Dvoráková M, "The effect of polyphenolic extract from pine bark, Pycnogenol on the level of glutathione in children suffering from attention deficit hyperactivity disorder(ADHD)", Redox Rep, 11, pp.163~172 (2006)
  33. El Adham EK, "Nutiritional and metabolic disturbances in attention deficit hyperactivity disease", Res J Med Med Sci, 6, pp.10~16 (2011)
  34. Ruchi K, "Antioxidant activity in children with ADHD-a comparison in untreated and treated subjects with normal children", IIUM Med J Malays, 10, pp.31~35 (2011)
  35. Selek S, "Evaluation of total oxidative status in adult attention deficit hyperactivity disorder and its diagnostic implications", J Psychiatr Res, 46, pp.451~455 (2012)
  36. Guney E, "Attention Deficit Hyperactivity Disorder and oxidative stress : a short term follow up study", Psychiatry Res, 229, pp.310~317 (2015)
  37. Nasim S, "Relationship between antioxidant status and attention deficit hyperactivity disorder among children", Int J Prev Med, 10, pp.41~ (2019)
  38. Haddad JJ, "Cytokines and neuro-immune-endocrine interactions : a role for the hypothalamic-pituitary-adrenal revolving axis", J Neuroimmunol, 133, pp.1~19 (2002)
  39. Terawaki H, "The elevation of oxidative stress after the great East Japan earthquake", Clin Exp Nephrol, 16, pp.816~817 (2012)
  40. Ates I, "Determination of thiol", disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation, 2016, pp.51, 47~51
  41. Altıparmak IH, "The relation of serum thiol levels and thiol", disulphide homeostasis with the severity of coronary artery disease, 2016, pp.74, 1346~1353
  42. Gamze Erzin, "Thiol", Disulfide Homeostasis in Bipolar and Unipolar Depression, 2020, pp.18(3), 395~401[DOI]
  43. Ünal K, "Thiol", disulphide homeostasis in schizophrenia patients with positive symptoms, 2018, pp.72, 281~284
  44. Simsek S, "Antioxidant status and DNA damage in children with attention deficit hyperactivity disorder with or without comorbid disruptive behavioral disorders", Bull Clin Psychopharmacol, 26, pp.119~125 (2016)
  45. Verlaet AAJ, "Oxidative stress and immune aberrancies in attention-deficit", hyperactivity disorder(ADHD) : a case-control comparison, 2019, pp.28, 719~729
  46. Che Y, "Oxidative damage to RNA but not DNA in the hippocampus of patients with major mental illness", J Psychiatry Neurosci, 35, pp.296~302 (2010)
  47. Nishioka N, "Evidence for oxidative DNA damage in the hippocampus of elderly patients with chronic schizophrenia", Am J Geriatr Psychiatry, 12, pp.167~175 (2004)
  48. Forlenza MJ, "Increased serum levels of 8-hydroxy-2'-deoxyguanosine in clinical depression", Psychosom Med, 68, pp.1~7 (2006)
  49. Ceprnja M, "Oxidative stress markers in patients with post-traumatic stress disorder", Coll Antropol, 35, pp.1155~1160 (2011)

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