본문 바로가기
KERD
논문KCI 등재

Feasibility of Using Serum, Plasma, and Platelet 5-hydroxytryptamine as Peripheral Biomarker for the Depression Diagnosis and Response Evaluation to Antidepressants: Animal Experimental Study

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Objective: Whether peripheral blood 5-hydroxytrptamine (5-HT) levels serve as biomarker for depression diagnosis/response evaluation has not been well determined. This work was explored to address this inconclusive issue. Methods: Animals were randomized into normal control group (NC, n = 10) and chronic unpredictable mild stress model group (CUMS-model, n = 20), respectively. Animals in CUMS-model group were subjected to chronic stress, then they were randomly subdivided into CUMS subgroup and CUMS + fluoxetine subgroup (CUMS + FLX). After FLX treatment, blood and tissues were collected. 5-HT and relevant protein expression were measured. Results: In mice model, there was a significant increase in serum and a significant reduction in plasma 5-HT levels in CUMS-model group versus NC group, while platelet 5-HT levels change little. After FLX treatment, serum and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup, while plasma 5-HT levels had not much change versus CUMS subgroup. Chronic stress enhanced colon and platelet serotonin transporter (SERT) expression and FLX treatment mitigated SERT expression. In rats’ model, there was a significant increase in serum 5-HT levels while plasma and platelet 5-HT levels showed little change in CUMS group versus NC group. After FLX treatment, serum, plasma and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup versus CUMS subgroup. The profile of relevant proteins expression changed by FLX were like those in mice. Conclusion: Serum 5-HT levels might serve as a potential biomarker for depression diagnosis, meanwhile serum and platelet 5-HT levels might respond to antidepressant treatment.

Abstract

Objective: Whether peripheral blood 5-hydroxytrptamine (5-HT) levels serve as biomarker for depression diagnosis/response evaluation has not been well determined. This work was explored to address this inconclusive issue. Methods: Animals were randomized into normal control group (NC, n = 10) and chronic unpredictable mild stress model group (CUMS-model, n = 20), respectively. Animals in CUMS-model group were subjected to chronic stress, then they were randomly subdivided into CUMS subgroup and CUMS + fluoxetine subgroup (CUMS + FLX). After FLX treatment, blood and tissues were collected. 5-HT and relevant protein expression were measured. Results: In mice model, there was a significant increase in serum and a significant reduction in plasma 5-HT levels in CUMS-model group versus NC group, while platelet 5-HT levels change little. After FLX treatment, serum and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup, while plasma 5-HT levels had not much change versus CUMS subgroup. Chronic stress enhanced colon and platelet serotonin transporter (SERT) expression and FLX treatment mitigated SERT expression. In rats’ model, there was a significant increase in serum 5-HT levels while plasma and platelet 5-HT levels showed little change in CUMS group versus NC group. After FLX treatment, serum, plasma and platelet 5-HT levels were significantly decreased in CUMS + FLX subgroup versus CUMS subgroup. The profile of relevant proteins expression changed by FLX were like those in mice. Conclusion: Serum 5-HT levels might serve as a potential biomarker for depression diagnosis, meanwhile serum and platelet 5-HT levels might respond to antidepressant treatment.

참고문헌 (54)

  1. "Depressive disorder (depression)" (2023)[URL]
  2. Harsanyi S, "Selected biomarkers of depression : what are the effects of cytokines and inflammation?", Int J Mol Sci, 24, pp.578~ (2022)
  3. Jesulola E, "Understanding the pathophysiology of depression : from monoamines to the neurogenesis hypothesis model-are we there yet?", Behav Brain Res, 341, pp.79~90 (2018)
  4. Tsai TY, "The interaction of oxytocin and social support, loneliness, and cortisol level in major depression", Clin Psychopharmacol Neurosci, 17, pp.487~494 (2019)
  5. Eun TK, "Association between the 5-HTTLPR genotype and childhood characteristics in mood disorders", Clin Psychopharmacol Neurosci, 14, pp.88~95 (2016)
  6. Coppen A, "The biochemistry of affective disorders", Br J Psychiatry, 113, pp.1237~1264 (1967)
  7. Albert PR, "The neurobiology of depression--revisiting the serotonin hypothesis. I. Cellular and molecular mechanisms", Philos Trans R Soc Lond B Biol Sci, 367, pp.2378~2381 (2012)
  8. Hahn A, "Attenuated serotonin transporter association between dorsal raphe and ventral striatum in major depression", Hum Brain Mapp, 35, pp.3857~3866 (2014)
  9. Yohn CN, "The role of 5-HT receptors in depression", Mol Brain, 10, pp.28~ (2017)
  10. Meyer JH, "Imaging the serotonin transporter during major depressive disorder and antidepressant treatment", J Psychiatry Neurosci, 32, pp.86~102 (2007)
  11. Smith K, "Mental health : a world of depression", Nature, 515, pp.181~ (2014)
  12. Malik S, "Biomarkers of major depressive disorder : knowing is half the battle", Clin Psychopharmacol Neurosci, 19, pp.12~25 (2021)
  13. Ogawa S, "Cerebrospinal fluid monoamine metabolite concentrations in depressive disorder : a meta-analysis of historic evidence", J Psychiatr Res, 105, pp.137~146 (2018)
  14. Sarrias MJ, "Decreased plasma serotonin in melancholic patients : a study with clomipramine", Biol Psychiatry, 22, pp.1429~1438 (1987)
  15. Choi W, "Interaction effect of serum serotonin level and age on the 12-week pharmacotherapeutic response in patients with depressive disorders", Sci Rep, 11, pp.24226~ (2021)
  16. Mück-Seler D, "The effects of paroxetine and tianeptine on peripheral biochemical markers in major depression", Prog Neuropsychopharmacol Biol Psychiatry, 26, pp.1235~1243 (2002)
  17. Liu MY, "Pretreatment with ginseng fruit saponins affects serotonin expression in an experimental comorbidity model of myocardial infarction and depression", Aging Dis, 7, pp.680~686 (2016)
  18. Kudryashov NV, "The behavioral and neurochemical aspects of the interaction between antidepressants and unpredictable chronic mild stress", Acta Naturae, 12, pp.63~72 (2020)
  19. Li C, "Could peripheral 5-HT level be used as a biomarker for depression diagnosis and treatment? A narrative minireview", Front Pharmacol, 14, pp.1149511~ (2023)
  20. Yang Z, "Antitumor effect of fluoxetine on chronic stress-promoted lung cancer growth via suppressing kynurenine pathway and enhancing cellular immunity", Front Pharmacol, 12, pp.685898~ (2021)
  21. Li G, "Effects of EGCG on depression-related behavior and serotonin concentration in a rat model of chronic unpredictable mild stress", Food Funct, 11, pp.8780~8787 (2020)
  22. Moncrieff J, "The serotonin theory of depression : a systematic umbrella review of the evidence", Mol Psychiatry, 28, pp.3243~3256 (2023)
  23. Nollet M, "Models of depression : unpredictable chronic mild stress in mice", Curr Protoc, 1, pp.e208~ (2021)
  24. Wang LS, "LC-MS", MS-based quantification of tryptophan metabolites and neurotransmitters in the serum and brain of mice, 2019, pp.1112, 24~32
  25. Willner P, "The chronic mild stress(CMS)model of depression : history, evaluation and usage", Neurobiol Stress, 6, pp.78~93 (2016)
  26. Antoniuk S, "Chronic unpredictable mild stress for modeling depression in rodents : meta-analysis of model reliability", Neurosci Biobehav Rev, 99, pp.101~116 (2019)
  27. Ferrari AJ, "Global variation in the prevalence and incidence of major depressive disorder : a systematic review of the epidemiological literature", Psychol Med, 43, pp.471~481 (2013)
  28. Lindström M, "Comparison of serum serotonin and serum 5-HIAA LC-MS", MS assays in the diagnosis of serotonin producing neuroendocrine neoplasms : a pilot study, 2018, pp.482, 78~83
  29. Sorge RE, "Different immune cells mediate mechanical pain hypersensitivity in male and female mice", Nat Neurosci, 18, pp.1081~1083 (2015)
  30. Audhya T, "Correlation of serotonin levels in CSF, platelets, plasma, and urine", Biochim Biophys Acta, 1820, pp.1496~1501 (2012)
  31. Fajardo O, "Serotonin, serotonin 5-HT1A receptors and dopamine in blood peripheral lymphocytes of major depression patients", Int Immunopharmacol, 3, pp.1345~1352 (2003)
  32. Matthes S, "Peripheral serotonin synthesis as a new drug target", Trends Pharmacol Sci, 39, pp.560~572 (2018)
  33. Scotton WJ, "Serotonin syndrome : pathophysiology, clinical features, management, and potential future directions", Int J Tryptophan Res, 12, pp.1178646919873925~ (2019)
  34. Fakhoury M, "New insights into the neurobiological mechanisms of major depressive disorders", Gen Hosp Psychiatry, 37, pp.172~177 (2015)
  35. Bianchi M, "Forced swimming test and fluoxetine treatment : in vivo evidence that peripheral 5-HT in rat platelet-rich plasma mirrors cerebral extracellular 5-HT levels, whilst 5-HT in isolated platelets mirrors neuronal 5-HT changes", Exp Brain Res, 143, pp.191~197 (2002)
  36. Fakhoury M, "Revisiting the serotonin hypothesis : implications for major depressive disorders", Mol Neurobiol, 53, pp.2778~2786 (2016)
  37. Anderson GM, "Determination of serotonin in whole blood, platelet-rich plasma, platelet-poor plasma and plasma ultrafiltrate", Life Sci, 40, pp.1063~1070 (1987)
  38. Birkenhäger TK, "Lithium addition in antidepressantresistant depression : effects on platelet 5-HT, plasma 5-HT and plasma 5-HIAA concentration", Prog Neuropsychopharmacol Biol Psychiatry, 31, pp.1084~1088 (2007)
  39. Brand T, "The measurement of platelet-poor plasma serotonin : a systematic review of prior reports and recommendations for improved analysis", Clin Chem, 57, pp.1376~1386 (2011)
  40. Todrick A, "Blood platelet 5-hydroxytryptamine levels in psychiatric patients", J Ment Sci, 106, pp.884~890 (1960)
  41. Boullin DJ, "Abnormalities in platelet 5-hydroxytryptamine efflux in patients with infantile autism", Nature, 226, pp.371~372 (1970)
  42. Boullin DJ, "Accumulation of dopamine by blood platelets from normal subjects and parkinsonian patients under treatment with L-DOPA", Br J Pharmacol, 39, pp.779~788 (1970)
  43. Karatoprak S, "Neutrophillymphocyte and platelet-lymphocyte ratios among adolescents with substance use disorder : a preliminary study", Clin Psychopharmacol Neurosci, 19, pp.669~676 (2021)
  44. Cıcek E, "Increased neutrophil-lymphocyte and platelet-lymphocyte ratios in male heroin addicts : a prospective controlled study", Clin Psychopharmacol Neurosci, 16, pp.190~196 (2018)
  45. Musselman DL, "Exaggerated platelet reactivity in major depression", Am J Psychiatry, 153, pp.1313~1317 (1996)
  46. Lima L, "Allelic isoforms and decrease in serotonin transporter mRNA in lymphocytes of patients with major depression", Neuroimmunomodulation, 12, pp.299~306 (2005)
  47. Franke L, "Serotonergic platelet variables in unmedicated patients suffering from major depression and healthy subjects : relationship between 5HT content and 5HT uptake", Life Sci, 67, pp.301~315 (2000)
  48. Schneider MA, "Attenuation of peripheral serotonin inhibits tumor growth and enhances immune checkpoint blockade therapy in murine tumor models", Sci Transl Med, 13, pp.eabc8188~ (2021)
  49. Li X, "Decreased platelet 5-hydroxytryptamin(5-HT)levels : a response to antidepressants", J Affect Disord, 187, pp.84~90 (2015)
  50. Alvarez JC, "Plasma serotonin level after 1 day of fluoxetine treatment : a biological predictor for antidepressant response?", Psychopharmacology(Berl), 143, pp.97~101 (1999)
  51. Biomarkers Definitions Working Group, "Biomarkers and surrogate endpoints : preferred definitions and conceptual framework", Clin Pharmacol Ther, 69, pp.89~95 (2001)
  52. Cagney DN, "The FDA NIH Biomarkers, EndpointS, and other Tools(BEST)resource in neuro-oncology", Neuro Oncol, 20, pp.1162~1172 (2018)
  53. Gafarov VV, "Arterial hypertension, myocardial infarction and stroke : risk of development and psychosocial factors", Alaska Med, 49(2 Suppl), pp.117~119 (2007)
  54. Gabriele S, "Blood serotonin levels in autism spectrum disorder : a systematic review and meta-analysis", Eur Neuropsychopharmacol, 24, pp.919~929 (2014)

관련 논문AI추천

Platelet Serotonin Concentration Is Associated with Illness Duration in Schizophrenia and Chronological Age in DepressionGetaldić-Švarc Biserka, Karlović Dalibor, Peitl Vjekoslav대한신경정신의학회 · PSYCHIATRY INVESTIGATION2020 · 제17권 제6호Associations of Serum Serotonin Levels with 12-week and 12-month Remission in Patients with Depressive Disorders강희주, Hee Kyung Kim, Ho-Cheol Kang, Jae-Min Kim, Ju-Wan Kim, Ju-Yeon Lee, Robert Stewart, Sung-Wan Kim, Wonsuk Choi대한정신약물학회 · Clinical Psychopharmacology and Neuroscience2022 · 제20권 제2호Use of Serum Biomarkers to Aid Antidepressant Selection in Depressive Patients강희주, Il-Seon Shin, Jae-Min Kim, Ju-Wan Kim, Ju-Yeon Lee, Sung-Wan Kim, Wonsuk Choi대한정신약물학회 · Clinical Psychopharmacology and Neuroscience2024 · 제22권 제1호Serum N-Desmethylcitalopram Concentrations are Associated with the Clinical Response to Citalopram of Patients with Major DepressionBerna Yucel, Canan Uluoglu, Gul Ozbey, Ismail Cuneyt Guzey, Nazan Cerit, Nevzat Yuksel, Nurdan Eren Bodur, Serap Erdogan Taycan, Tayyibe Arslan대한신경정신의학회 · PSYCHIATRY INVESTIGATION2018 · 제15권 제3호Effects of 5HT1A Activation on Depression Profile Following 5-HT Depletion in Rats Lacking Social Attachment Since WeanlingChen-Cheng Lin, Jing-Yi Chung, Kuo-Jung Chang, Yia-Ping Liu, Yu-Jung Chen대한신경정신의학회 · PSYCHIATRY INVESTIGATION2018 · 제15권 제10호Biomarkers in Drug Free Subjects with Depression : Correlation with TryptophanFarzana Yasmin, Natasha Manzoor, Noreen Samad대한신경정신의학회 · PSYCHIATRY INVESTIGATION2019 · 제16권 제12호

이 간행물의 다른 논문 (15건)