Influenza M1 Virus-Like Particles Consisting of Toxoplasma gondii Rhoptry Protein 4
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs) consisting of T. gondii rhoptry antigen 4 (ROP4 protein) were generated using baculovirus (rBV) expression system. Recombinant ROP4 protein with influenza M1 were cloned and expressed in rBV. SF9 insect cells were coinfected with recombinant rBVs expressing T. gondii ROP4 and influenza M1. As the results, influenza M1 VLPs showed spherical shapes, and T. gondii ROP4 protein exhibited as spikes on VLP surface under transmission electron microscopy (TEM). The M1 VLPs resemble virions in morphology and size. We found that M1 VLPs reacted with antibody from T. gondii-infected mice by western blot and ELISA. This study demonstrated that T. gondii ROP4 protein can be expressed on the surface of influenza M1 VLPs and the M1 VLPs containing T. gondii ROP4 reacted with T. gondii-infected sera, indicating the possibility that M1 VLPs could be used as a coating antigen for diagnostic and/or vaccine candidate against T. gondii infection.
Abstract
Toxoplasma gondii infections occur throughout the world, and efforts are needed to develop various vaccine candidates expressing recombinant protein antigens. In this study, influenza matrix protein (M1) virus-like particles (VLPs) consisting of T. gondii rhoptry antigen 4 (ROP4 protein) were generated using baculovirus (rBV) expression system. Recombinant ROP4 protein with influenza M1 were cloned and expressed in rBV. SF9 insect cells were coinfected with recombinant rBVs expressing T. gondii ROP4 and influenza M1. As the results, influenza M1 VLPs showed spherical shapes, and T. gondii ROP4 protein exhibited as spikes on VLP surface under transmission electron microscopy (TEM). The M1 VLPs resemble virions in morphology and size. We found that M1 VLPs reacted with antibody from T. gondii-infected mice by western blot and ELISA. This study demonstrated that T. gondii ROP4 protein can be expressed on the surface of influenza M1 VLPs and the M1 VLPs containing T. gondii ROP4 reacted with T. gondii-infected sera, indicating the possibility that M1 VLPs could be used as a coating antigen for diagnostic and/or vaccine candidate against T. gondii infection.
참고문헌 (29)
- Dubey JP, "Toxoplasmosis of Animals and Humans" (2010), CRC
- Robert-Gangneux F, "Epidemiology of and diagnostic strategies for toxoplasmosis", Clin Microbiol Rev, 25, pp.264~296 (2012)
- Chen J, "Canine and feline parasitic zoonoses in China", Parasit Vectors, 5, pp.152~ (2012)
- Lee DH, "Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii", PLoS One, 11, pp.e0161231~ (2016)
- Hill D, "Toxoplasma gondii: transmission, diagnosis and prevention", Clin Microbiol Infect, 8, pp.634~640 (2002)
- Liu Q, "Diagnosis of toxoplasmosis and typing of Toxoplasma gondii", Parasit Vectors, 8, pp.292~ (2015)
- Liesenfeld O, "False-positive results in immunoglobulin M (IgM) toxoplasma antibody tests and importance of confirmatory testing: the Platelia Toxo IgM test", J Clin Microbiol, 35, pp.174~178 (1997)
- Wilson M, "Evaluation of six commercial kits for detection of human immunoglobulin M antibodies to Toxoplasma gondii. The FDA Toxoplasmosis Ad Hoc Working Group", J Clin Microbiol, 35, pp.3112~3115 (1997)
- Kotresha D, "Recombinant proteins in the diagnosis of toxoplasmosis", APMIS, 118, pp.529~542 (2010)
- Lau YL, "Cloning and expression of Toxoplasma gondii dense granule antigen 2(GRA2)gene by Pichia pastoris", Southeast Asian J Trop Med Public Health, 43, pp.10~ (2012)
- Thiruvengadam G, "Optimization of the expression of surface antigen SAG1", 2 of Toxoplasma gondii in the yeast Pichia pastoris, 2011, pp.28, 506~513
- Chang PY, "Evaluation of Pichia pastoris-expressed recombinant rhoptry protein 2 of Toxoplasma gondii for its application in diagnosis of toxoplasmosis", Am J Trop Med Hyg, 85, pp.485~489 (2011)
- Wang Z, "Evaluation of recombinant granule antigens GRA1 and GRA7 for serodiagnosis of Toxoplasma gondii infection in dogs", BMC Vet Res, 10, pp.158~ (2014)
- Wang Z, "Production and evaluation of recombinant granule antigen protein GRA7 for serodiagnosis of Toxoplasma gondii infection in cattle", Foodborne Pathog Dis, 11, pp.734~739 (2014)
- Lee DH, "Cross-protection induced by Toxoplasma gondii virus-like particle vaccine upon intraperitoneal route challenge", Acta Trop, 164, pp.77~83 (2016)
- Counihan NA, "Plasmodium rhoptry proteins : why order is important", Trends Parasitol, 29, pp.228~236 (2013)
- Dubremetz JF, "Rhoptries are major players in Toxoplasma gondii invasion and host cell interaction", Cell Microbiol, 9, pp.841~848 (2007)
- Wang PY, "Protective efficacy of a Toxoplasma gondii rhoptry protein 13 plasmid DNA vaccine in mice", Clin Vaccine Immunol, 19, pp.1916~1920 (2012)
- Joyce BR, "Phosphorylation of eukaryotic initiation factor-2α promotes the extracellular survival of obligate intracellular parasite Toxoplasma gondii", Proc Natl Acad Sci USA, 107, pp.17200~17205 (2010)
- Chen J, "Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice", Vaccine, 31, pp.1734~1739 (2013)
- Quan FS, "Virus-like particle vaccine induces protective immunity against homologous and heterologous strains of influenza virus", J Virol, 81, pp.3514~3524 (2007)
- Quan FS, "Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice", J Infect Dis, 204, pp.987~995 (2011)
- Letscher-Bru V, "Vaccination with Toxoplasma gondii SAG-1 protein is protective against congenital toxoplasmosis in BALB", c mice but not in CBA, Infect Immun, pp.2003, 71[DOI]
- Dlugonska H, "Toxoplasma rhoptries: unique secretory organelles and source of promising vaccine proteins for immunoprevention of toxoplasmosis", J Biomed Biotechnol, pp.e632424~ (2008)
- Holec-Gąsior, L, "Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies", Clin Vaccine Immunol, 20, pp.1343~1351 (2013)
- Jongert E, "Vaccines against Toxoplasma gondii : challenges and opportunities", Mem Inst Oswaldo Cruz, 104, pp.252~266 (2009)
- Dziadek B, "Toxoplasma gondii: the vaccine potential of three trivalent antigen-cocktails composed of recombinant ROP2, ROP4, GRA4 and SAG1 proteins against chronic toxoplasmosis in BALB", c mice, 2012, pp.131, 133~138
- Dziadek B, "Toxoplasma gondii : the immunogenic and protective efficacy of recombinant ROP2 and ROP4 rhoptry proteins in murine experimental toxoplasmosis", Exp Parasitol, 123, pp.81~89 (2009)
- Gatkowska J, "Determination of diagnostic value of Toxoplasma gondii recombinant ROP2 and ROP4 antigens in mouse experimental model", Pol J Microbiol, 59, pp.137~141 (2010)