Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions
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초록
Multipurpose contact lens disinfecting solutions (MPDS) are widely used to cleanse and disinfect microorgan- isms. However, disinfection efficacy of these MPDS against Acanthamoeba cyst remain insufficient. 2, 6-dichlorobenzoni- trile (DCB), a cellulose synthesis inhibitor, is capable of increasing the amoebical effect against Acanthamoeba by inhibit- ing its encystation. In this study, we investigated the possibility of DCB as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba cyst. Eight commercial MPDS (from a to h) were assessed, all of which displayed insufficient amoebicidal activity against the mature cysts. Solution e, f, and h showed strong amoebicidal effect on the im- mature cysts. Amoebicidal efficacy against mature cysts remained inadequate even when the 8 MPDS were combined with 100 μM DCB. However, 4 kinds of MPDS (solution d, e, f, and h) including 100 μM DCB demonstrated strong amoe- bicidal activity against the immature cysts. The amoebicidal activity of solution d was increased by addition of DCB. Cy- totoxicity was absent in human corneal epithelial cells treated with either DCB or mixture of DCB with MPDS. These re- sults suggested that DCB can enhance the amoebicical activity of MPDS against Acanthamoeba immature cyst in vitro.
Abstract
Multipurpose contact lens disinfecting solutions (MPDS) are widely used to cleanse and disinfect microorgan- isms. However, disinfection efficacy of these MPDS against Acanthamoeba cyst remain insufficient. 2, 6-dichlorobenzoni- trile (DCB), a cellulose synthesis inhibitor, is capable of increasing the amoebical effect against Acanthamoeba by inhibit- ing its encystation. In this study, we investigated the possibility of DCB as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba cyst. Eight commercial MPDS (from a to h) were assessed, all of which displayed insufficient amoebicidal activity against the mature cysts. Solution e, f, and h showed strong amoebicidal effect on the im- mature cysts. Amoebicidal efficacy against mature cysts remained inadequate even when the 8 MPDS were combined with 100 μM DCB. However, 4 kinds of MPDS (solution d, e, f, and h) including 100 μM DCB demonstrated strong amoe- bicidal activity against the immature cysts. The amoebicidal activity of solution d was increased by addition of DCB. Cy- totoxicity was absent in human corneal epithelial cells treated with either DCB or mixture of DCB with MPDS. These re- sults suggested that DCB can enhance the amoebicical activity of MPDS against Acanthamoeba immature cyst in vitro.
참고문헌 (13)
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