Down-regulation of cellulose synthase inhibits the formation of endocysts in Acanthamoeba
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.
Abstract
Acanthamoeba cysts are resistant to unfavorable physiological conditions and various disinfectants. Acanthamoeba cysts have 2 walls containing various sugar moieties, and in particular, one third of the inner wall is composed of cellulose. In this study, it has been shown that down-regulation of cellulose synthase by small interfering RNA (siRNA) significantly inhibits the formation of mature Acanthamoeba castellanii cysts. Calcofluor white staining and transmission electron microscopy revealed that siRNA transfected amoeba failed to form an inner wall during encystation and thus are likely to be more vulnerable. In addition, the expression of xylose isomerase, which is involved in cyst wall formation, was not altered in cellulose synthase down-regulated amoeba, indicating that cellulose synthase is a crucial factor for inner wall formation by Acanthamoeba during encystation.
참고문헌 (21)
- Marciano-Cabral F, "Acanthamoeba spp. as agents of disease in humans", Clin Microbiol Rev, 16, pp.273~307 (2003)
- 문은경, "Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii", The Korean Journal of Parasitology, 49(4), pp.341~347 (2011), 대한기생충학ㆍ열대의학회
- Bouyer S, "Acanthamoeba castellanii : proteins involved in actin dynamics, glycolysis, and proteolysis are regulated during encystation", Exp Parasitol, 123, pp.90~94 (2009)
- Moon EK, "Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba", Mol Biochem Parasitol, 168, pp.43~48 (2009)
- Song SM, "Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii", Mol Biochem Parasitol, 183, pp.158~165 (2012)
- Leitsch D, "Major role for cysteine proteases during the early phase of Acanthamoeba castellanii encystment", Eukaryot Cell, 9, pp.611~618 (2010)
- 문은경, "Differentially expressed genesof Acanthamoeba castellanii during encystation", The Korean Journal of Parasitology, 45(4), pp.283~285 (2007), 대한기생충학ㆍ열대의학회
- Moon EK, "Characterization of a serine proteinase mediating encystation of Acanthamoeba", Eukaryot Cell, 7, pp.1513~1517 (2008)
- Moon EK, "Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba", Mol Biochem Parasitol, 185, pp.121~126 (2012)
- Bowers B, "The fine structure of Acanthamoeba castellanii (Neff strain). II. Encystment", J Cell Biol, 41, pp.786~805 (1969)
- Neff RJ, "The biochemistry of amoebic encystment", Symp Soc Exp Biol, 23, pp.51~81 (1969)
- Linder M, "Use of recombinant cellulose-binding domains of Trichoderma reesei cellulase as a selective immunocytochemical marker for cellulose in protozoa", Appl Environ Microbiol, 68, pp.2503~2508 (2002)
- Dudley R, "Cellulose biosynthesis pathway is a potential target in the improved treatment of Acanthamoeba keratitis", Appl Microbiol Biotechnol, 75, pp.133~140 (2007)
- Derda M, "Use of the presence of cellulose in cellular wall of Acanthamoeba cysts for diagnostic purposes", Wiad Parazytol, 55, pp.47~51 (2009)
- Lorenzo-Morales J, "Glycogen phosphorylase in Acanthamoeba spp.: determining the role of the enzyme during the encystment process using RNA interference", Eukaryot Cell, 7, pp.509~517 (2008)
- 문은경, "Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba", The Korean Journal of Parasitology, 50(4), pp.361~364 (2012), 대한기생충학ㆍ열대의학회
- Aqeel Y, "Silencing of xylose isomerase and cellulose synthase by siRNA inhibits encystation in Acanthamoeba castellanii", Parasitol Res, 112, pp.1221~1227 (2013)
- Aksozek A, "Resistance of Acanthamoeba castellanii cysts to physical, chemical, and radiological conditions", J Parasitol, 88, pp.621~623 (2002)
- Khunkitti W, "Biguanide-induced changes in Acanthamoeba castellanii : an electron microscopic study", J Appl Microbiol, 84, pp.53~62 (1998)
- Dudley R, "Carbohydrate analysis of Acanthamoeba castellanii", Exp Parasitol, 122, pp.338~343 (2009)
- Tomlinson G, "Isolation of cellulose from the cyst wall of a soil amoeba", Biochim et Biophys Acta, 63, pp.194~200 (1962)