단순포진 바이러스 검출 및 형결정을 위한 간접면역형광법과 Nested Multiplex-중합효소연쇄반응의 비교
A Comparison of Nested Multiplex-Polymerase Chain Reaction with IndirectImmunofluorescence for Detection and Typing of Herpes Simplex Virus
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목적 : HSV-1은 주로 구강 및 구순에 감염을 일으키고, HSV-2는 생식기에 감염을 일으킨다. 연구자들은 HSV의 검출 및 형결정을 위한 Coyle 등의 nested multipex-polymerase chain reac-tion(NM-PCR) 방법을 평가하고자 이 연구를 실시하였으며, 세포배양 후 간접면역형광법의 결과와 비교 평가하였다. 방법 : HSV 감염이 의심되는 133 검체를 검사 대상으로 하였다. 검체는 shel vial을 이용한 세포배양 후 HSV-1 및 HSV-2 단클론항체를 이용하여 간접면역형광 염색을 실시하였다. 원래 검체는 HSV 검출 및 형결정 검사를 위하여 NM-PCR (J Virol Me -thods 1999; 83: 75-82)을 실시하였다. 결과 : 세포배양 후의 간접면역형광법은 45/133 (33.8%)예,NM-PCR은 46/133 (34.6%)예에서 HSV를 검출하였다. 간접면역형광법에서 HSV 양성인 검체는 모두 NM-PCR에서 양성이었다. 두 방법간에서 형결정 일치율은 45 검체 중 43 검체로 95.6%이었다. 나머지 2 검체는 간접면역형광법에서 각기 HSV-1과 HSV-2이었으나 NM-PCR에서는 HSV-1과 HSV-2 모두가 각 검체에서 동정되었다. 결론 : NM-PCR은 HSV감염 검출 및 형결정에 신속 정확한 검사방법이다.
Abstract
Background : Herpes simplex virus type 1 (HSV-1) is linked specifically to oral and lip infections while HSV-2 is involved in genital infections. We evaluated a recently reported nested-multiplex ploymerase chain reaction (NM-PCR) for the detection and typing of HSV and compared the results with indirect immunofluorescence (IF) after cell culture. Methods : One hundred thirty three specimens were received from patients suspected of having clinical HSV infections. HSV was cultured by the shell vial method and stained with type specific monoclonal antibodies. NM-PCR was performed using crude samples. Results : HSV was detected in 45 (33.8%) and 46 (34.6%) of the 133 specimens by IF and NMPCR, respectively. All of the HSV IF positive specimens were also positive by NM-PCR. Typing by the two methods concurred in all but two of the 45 specimens; the two specimens were typed as HSV-1 and HSN-2, respectivey, by IF, and both as HSV-1 and HSV-2 by NM-PCR. Conclusions : Our results suggest that NM-PCR is a rapid and sensitive method for the detection and typing of HSV. (Korean J Lab Med 2004; 24: 113-8)
참고문헌 (24)
- "Herpes simplex virus(HSV)분리에 관한연구", 19, pp.97~106 (1987)
- "Encephalitis inimmunocompetent patients due to Herpes simplex virus type 1 or2 as determinated by type-specific polymerase chain reaction andantibody assay of cerebrospinal fluid", 39, pp.179~86 (1993)
- "Derection of viral DNA in neonatal Herpes simplex virus infec-tions:Frequent and prolonged presence in serum and cerbrospinalfluid", 164, pp.289~93 (1991)
- "Nested polymerase chain reaction을이용한연령및감염부위별단순포진바이러스아형분류", 36, pp.643~651 (1998)
- "Shell vial culture를이용한단순포진바이러스분리", 23, pp.324~328 (2003)
- "The etiology of genital ulcer disease by mul-tiplex polymerase chain reaction and relationship to HIV infectionamong patients attending sexually transmitted disease clinics in Pune,India", 26, pp.55~62 (1999)
- "Recrudescent Herpes simple infection mimicking primary herpeticgingivostomitis", 27, pp.8~10 (1998)
- "Disseminated Herpes simplexvirus and Varicella zoster virus coinfection in a patient taking thalido-midefor relapsed multiple myeloma", 40, pp.2302~2304 (2002)
- "A compar-ison of virus isolation,indirect immunofluorescence and nested mul-tiplex polymerase chain reaction for the diagnosis of primary andrecurrent herpes simplex type 1 and type 2 infections", 83, pp.75~82 (1999)
- "Vidarabine theraphy of neonatal herpes simplex virus infection", 66, pp.495~501 (1980)
- "Laboratory techniques in the diagnosis of herpes simpleinfection", 69, pp.174~83 (1993)
- "Evaluation of three immunoflu-orecenceassay for culture confirmation and typing of Herpes sim-plex virus", 25, pp.391~4 (1987)
- "Comparative evaluation of four com-mercially available monoclonal antibodies for culture confirmationof Herpes simplex virus infection", 30, pp.1874~5 (1992)
- "Evaluationof a direct immunofluorescence cytospin assay for the detection ofHerpes simplex virus in clinical samples", 16, pp.851~854 (1997)
- "Detection and typing of HSV-1,HSV-2,andVZV by a multiplex polymerase chain reaction", 14, pp.214~219 (2000)
- "Evaluation of a newgeneral primer pair for rapid detection and differentiation of HSV-1,HSV-2,and VZV by polymerase chain reaction", 49, pp.279~282 (1996)
- "Typ-ing of clinical isolates with mouse monoclonal antibodies to herpessimplex virus types 1 and 2:Comparison with type-specific rabbitantisera and restriction endonuclease analysis of virus DNA", 17, pp.92~6 (1983)
- "Utilityof restriction fragment analysis for typing herpes simplex virus ampli-con following PCR of targets in the DNA polymerase gene", 37, pp.289~291 (2000)
- "Herpes simplex virus and their replica-tion. In", pp.2231~2295 (1996), Lippincott-Raven
- "Urine specimens from pregnant and nonpregnant women inhibitoryto amplification of Chlamydia trachomatisnucleic acid by PCR,ligasechain reaction and transcription-mediated amplification:identifica-tion of urinary substances associated with inhibition and removal ofinhibitory activity", 36, pp.3122~3126 (1998)
- "Acomparison of PCR with virus isolation and direct antigen detectionfor diagnosis and typing of genital herpes", 55, pp.177~183 (1998)
- "Evidence for Herpes simplexvirus type-selective receptor on cellular plasma membranes", 44, pp.217~25 (1979)
- "Differencesin attachment between herpes simplex type 1 and type 2viruses at neurons and glial cells", 28, pp.675~80 (1980)
- "Evidence oflatency and reactivation of both herpes simplex virus -1 andHSV-2 in the genital region HSV-2 in the genital region", 177, pp.1069~1072 (1998)