정상 성인에서 단핵구 CD14/-159 C→T 다형성과 혈중 지단백 농도와의 연관성
Association of the CD14/-159 C→T Polymorphism with Variations in Serum Lipoproteins in Healthy Population
1
조회
0
다운로드
0
인용
0
참고문헌
초록
배경 : CD14는 지다당류에 대한 수용체로서 LPS에 의한 단핵구 활성과 염증반응 매개에 중요하다. 최근 CD14/-159 C→T 유전자다형성이 CD14 발현에 영향을 미치고 심혈관계질환을 비롯한 여러 질환과 연관성이 있음이 밝혀졌다. 저자들은 혈중 지단백 농도와 CD14/-159 유전형과의 연관성을 알아보고자 하였다. 방법 : 156명의 건강한 성인을 대상으로 혈중 총콜레스테롤, LDL, HDL, apo A1, apo B-100, 중성지방을 측정하였고, CD14/-159 유전형은 제한효소절편분석법으로 분석하였다. 결과 : 전체 유전형 빈도는 CC 13.5%, TT 35.9%, CT 50.6%, T 유전형 빈도는 61.2%였으며, 각 유전형과 지단백 농도와의 연관성은 찾을 수 없었다(P>0.05). 결론 : CD14/-159 유전형과 혈중 지단백과의 유의한 연관성은 보이지 않았으며, 향후 더 많은 연구가 필요할 것으로 생각된다.
Abstract
Background : The CD14 receptor is an important mediator for the activation of monocyte by lipopolysaccharides. Recently described data indicate that the CD14/-159 C→T polymorphism is associated with elevated CD14 expression, decreased IgE and increased myocardial infarction risk. In the present study, we investigated the CD14/-159 C→T polymorphism to determine its association with lipid profile in healthy adults. Methods : The CD14 promoter genotype was determined by polymerase chain reaction amplification- restriction fragment length polymorphism analysis in 156 healthy adults. Results of the CD14 promotor genotype were compared with total cholesterol, LDL, HDL, apo A1, apo B100, and triglyceride using one way ANOVA and independent T tests. Results : The genotype frequencies were CC 13.5%, TT 35.9%, CT 50.6% and the T allele frequency was 61.2%; there was no association between CD14 promotor genotype and lipoprotein concentrations (P>0.05). Conclusions : Our results indicate that CD14/-159 C→T polymorphism is not associated with serum lipoprotein concentrations
참고문헌 (10)
- "The CD14 monocyte differentiation antigen maps to a regionencoding growth factors and receptors", 239, pp.497~500 (1988)
- "Mono-cyteantigen CD14 is a phospholipid anchored membrane protein", 73, pp.284~9 (1989)
- "Biochemical characterization of a soluble form of the 53-kDamonocytesurface antigen", 16, pp.1583~9 (1986)
- "a receptor for complexes of lipopolysaccharide", pp.1431~3
- "Lipopolysaccharide(LPS)-binding protein acceler-ates the binding of LPS to CD14", 179, pp.269~77 (1994)
- "The role of CD14 andlipopolysaccharide-binding protein(LBP)in the activation of differentcell typesby endotoxin", 183, pp.279~97 (1994)
- "CD14 is a com-ponent of multiple recognition systems used by macrophages to pha-gocytose apoptotic lymphocytes", 6, pp.583~92 (1999)
- "Neutraliza-tion and transfer of lipopolysaccharide by phospholipid transferprotein", 271, pp.12172~8 (1996)
- "Lipopo-lysaccharide-binding protein and phospholipid transfer protein releaselipopolysaccharides from gram-negative bacterial membranes", 68, pp.2410~7 (2000)
- "Association of the -159 C→T polymorphism in the CD14 promoter with variations in serumlipoproteins in healthy subjects", 14, pp.663~70 (2003)