Excretion of Toxoplasma gondii oocysts from Feral Cats in Korea
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Sporulated oocysts from the feces of infected cats with Toxoplasma gondii can cause detrimental disease in both humans and animals. To investigate the prevalence of feral cats that excrete T. gondii oocysts in the feces, we examined fecal samples of 563 feral cats over a 3-year period from 2009 to 2011. Oocysts of T. gondii excreted into the feces were found from 4 of 128 cats in 2009 (3.1%) and one of 228 (0.4%) in 2010 while none of the 207 cats in 2010 were found positive with oocysts in their feces, resulting in an overall prevalence rate of 0.89% (5/563) between 2009 and 2011. Among the 5 cats that tested positive with T. gondii oocysts, 4 of the cats were male and 1 was a female with an average body weight of 0.87 kg. Numerous tissue cysts of 60 μm in diameter with thin (<0.5 μm) cyst walls were found in the brain of one of the 5 cats on necropsy 2 months after the identification of oocysts in the feces. A PCR amplification of the T. gondii-like oocysts in the feces of the positive cats using the primer pairs Tox-5/Tox-8 and Hham34F/Hham3R confirmed the presence of T. gondii oocysts in the feces. This study provides a good indication of the risk assessment of feral cats in the transmission of T. gondii to humans in Korea.
Abstract
Sporulated oocysts from the feces of infected cats with Toxoplasma gondii can cause detrimental disease in both humans and animals. To investigate the prevalence of feral cats that excrete T. gondii oocysts in the feces, we examined fecal samples of 563 feral cats over a 3-year period from 2009 to 2011. Oocysts of T. gondii excreted into the feces were found from 4 of 128 cats in 2009 (3.1%) and one of 228 (0.4%) in 2010 while none of the 207 cats in 2010 were found positive with oocysts in their feces, resulting in an overall prevalence rate of 0.89% (5/563) between 2009 and 2011. Among the 5 cats that tested positive with T. gondii oocysts, 4 of the cats were male and 1 was a female with an average body weight of 0.87 kg. Numerous tissue cysts of 60 μm in diameter with thin (<0.5 μm) cyst walls were found in the brain of one of the 5 cats on necropsy 2 months after the identification of oocysts in the feces. A PCR amplification of the T. gondii-like oocysts in the feces of the positive cats using the primer pairs Tox-5/Tox-8 and Hham34F/Hham3R confirmed the presence of T. gondii oocysts in the feces. This study provides a good indication of the risk assessment of feral cats in the transmission of T. gondii to humans in Korea.
참고문헌 (42)
- De Craeye S, "Prevalence of Toxoplasma gondii infection in Belgian house cats", Vet Parasitol, 157, pp.128~132 (2008)
- Dubey JP, "Toxoplasmosis of Animals and Man", pp.1~232 (1988), CRC Press
- Dubey JP, "Toxoplasmosis of Animals and Humans", pp.1~30 (2010), CRC Press
- Tenter AM, "Toxoplasma gondii : from animals to humans", Int J Parasitol, 30, pp.1217~1258 (2000)
- Bowie WR, "Outbreak of toxoplasmosis associated with municipal drinking water. The BC Toxoplasma Investigation Team", Lancet, 350, pp.173~177 (1997)
- Burnett AJ, "Multiple cases of acquired toxoplasmosis retinitis presenting in an outbreak", Ophthalmology, 105, pp.1032~1037 (1998)
- Dubey JP, "Toxoplasmosis–a waterborne zoonosis", Vet Parasitol, 126, pp.57~72 (2004)
- Schares G, "Characterization of a repetitive DNA fragment in Hammondia hammondi and its utility for the specific differentiation of H. hammondii from Toxoplasma gondii by PCR", Mol Cell Probes, 22, pp.244~251 (2008)
- Hill D, "Toxoplasma gondii : transmission, diagnosis and prevention", Clin Microbiol Infect, 8, pp.634~640 (2002)
- Han DU, "Serological studies on Toxoplasma gondii, Hantavirus and some rickettsial pathogens in stray cats in Korea", Korean J Vet Pub Health, 23, pp.301~310 (1999)
- 김혜연, "Prevalence of Toxoplasma gondii in Stray Cats of Gyeonggi-do, Korea", Parasites, Hosts and Diseases, 46(3), pp.199~201 (2008), 대한기생충학ㆍ열대의학회
- Lee SE, "Prevalence of Toxoplasma gondii Infection in Feral Cats in Seoul, Korea", J Parasitol, 97, pp.153~155 (2011)
- Vollaire MR, "Seroprevalence of Toxoplasma gondii antibodies in clinically ill cats in the United States", Am J Vet Res, 66, pp.874~877 (2005)
- Dubey JP, "Toxoplasma gondii infection in humans and animals in the United States", Int J Parasitol, 38, pp.1257~1278 (2008)
- Dubey JP, "Duration of immunity to shedding of Toxoplasma gondii oocysts by cats", J Parasitol, 81, pp.410~415 (1995)
- Torrence ME, "Microbial Food Safety in Animal Agriculture", pp.359~367 (2003), Iowa State Press
- Dubey JP, "Oocyst shedding by cats fed isolated bradyzoites and comparison of infectivity of bradyzoites of the VEG strain Toxoplasma gondii to cats and mice", J Parasitol, 87, pp.215~219 (2001)
- Dubey JP, "Cyst-induced toxoplasmosis in cats", J Protozool, 19, pp.155~177 (1972)
- Dubey J, "Sources and reservoirs of Toxoplasma gondii infection on 47 swine farms in Illinois", J Parasitol, pp.723~729 (1995)
- Aparicio Garrido J, "Estudios sobre la epidemiologia de la toxoplasmosis. La infeccion del gato domestico en los alrededores de Madrid. Encuestas serologica y coproparasitologica", Med Trop, 48, pp.24~39 (1972)
- Pampiglione S, "Toxoplasma gondii oocysts in the faeces of naturally infected cat", Br Med J, 2, pp.306~ (1973)
- Ito S, "Small type of Isospora bigemina : isolation from naturally infected cats and relations with toxoplasma oocyst", Natl Inst Anim Health Q, 14, pp.137~144 (1974)
- 이상은, "Prevalence of Toxoplasma gondii Infection in Stray and Household Cats in Regions of Seoul, Korea", Parasites, Hosts and Diseases, 48(3), pp.267~270 (2010), 대한기생충학ㆍ열대의학회
- Zajac AM, "Veterinary Clinical Parasitology", pp.4~12 (2012), Wiley-Blackwell
- Dubey JP, "The Toxoplasma gondii oocyst from cat feces", J Exp Med, 132, pp.636~662 (1970)
- Reischl U, "Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes", BMC infectious diseases, 3, pp.7~ (2003)
- Rifaat MA, "Toxoplasma infection of stray cats in Egypt", J Trop Med, 79, pp.67~70 (1976)
- Wallace GD, "Isolation of Toxoplasma gondii from the feces of naturally infected cats", J Infect Dis, 124, pp.227~228 (1971)
- Wallace GD, "The role of the cat in the natural history of Toxoplasma gondii", Am J Trop Med Hyg, 22, pp.313~ (1973)
- Nery-Guimaraes F, "Producao irregular e inconstante de oocistos pela ministracao de cistos de ‘‘Toxoplasma gondii’’Nicolle & Manceaux, 1909, em gatos", Mem Inst Oswaldo Cruz, 71, pp.157~170 (1973)
- Maruyama S, "Seroprevalence of Bartonella henselae, Toxoplasma gondii, FIV and FeLV infections in domestic cats in Japan", Microbiol Immunol, 47, pp.147~153 (2003)
- Miró G, "Prevalence of antibodies to Toxoplasma gondii and intestinal parasites in stray, farm and household cats in Spain", Vet Parasitol, 126, pp.249~255 (2004)
- Kapperud G, "Risk factors for Toxoplasma gondii infection in pregnancy results of a prospective case-control study in Norway", Am J Epidemiol, 144, pp.405~412 (1996)
- Cook AJ, "Sources of toxoplasma infection in pregnant women: European multicentre case-control study. European Research Network on Congenital Toxoplasmosis", BMJ, 321, pp.142~147 (2000)
- Soh CT, "Latent infection by Toxoplasma gondii in Korea", Yonsei Med J, 1, pp.52~54 (1960)
- Choi WY, "Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay", Korean J Parasitol, 30, pp.83~90 (1992)
- Yang HJ, "Seroprevalence of toxoplasmosis in the residents of Cheju island, Korea", Korean J Parasitol, 38, pp.91~93 (2000)
- 송경주, "Seroprevalence of toxoplasmosis in Korean pregnant women", Parasites, Hosts and Diseases, 43(2), pp.1~10 (2005), 대한기생충학ㆍ열대의학회
- Han K, "Seroprevalence of Toxoplasma gondii infection and risk factors associated with seropositivity of pregnant women in Korea", J Parasitol, 94, pp.963~965 (2008)
- 신대환, "Seroprevalence of Toxoplasma gondii Infection and Characteristics of Seropositive Patients in General Hospitals in Daejeon, Korea", Parasites, Hosts and Diseases, 47(2), pp.125~130 (2009), 대한기생충학ㆍ열대의학회
- Dorny P, "Serological survey of Toxoplasma gondii, feline immunodeficiency virus and feline leukaemia virus in urban stray cats in Belgium", Vet Rec, 151, pp.626~629 (2002)
- NVRQS, "A public opinion survey on animal protection in Korea", J Korean Vet Med Assoc, 43, pp.225~236 (2007)
