Toxoplasma gondii B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The increasing prevalence of Toxoplasma gondii infection in the human population in the Republic of Korea (= Korea) is due to various reasons such as an increase in meat consumption. However, the importance of cats in transmitting T. gondii infection through oocysts to humans has seldom been assessed. A total of 300 fecal samples of stray cats captured around Seoul from June to August 2013 were examined for T. gondii B1 gene (indicating the presence of oocysts) using nested-PCR. Fourteen (4.7%) of 300 cats examined were positive for B1 gene. Female cats (7.5%) showed a higher prevalence than male cats (1.4%). Cats younger than 3 months (5.5%) showed a higher prevalence than cats (1.5%) older than 3 months. For laboratory passage of the positive samples, the fecal suspension (0.2 ml) of B1 gene positive cats was orally inoculated into experimental mice. Brain tissues of the mice were obtained after 40 days and examined for the presence of tissue cysts. Two isolates were successfully passaged (designated KNIH-1 and KNIH-2) and were molecularly analyzed using the SAG5D and SAG5E gene sequences. The SAG5D and SAG5E gene sequences showed high homologies with the ME49 strain (less virulent strain). The results indicated the importance of stray cats in transmitting T. gondii to humans in Korea, as revealed by detection of B1 gene in fecal samples. T. gondii isolates from cats were successfully passaged in the laboratory for the first time in Korea.
Abstract
The increasing prevalence of Toxoplasma gondii infection in the human population in the Republic of Korea (= Korea) is due to various reasons such as an increase in meat consumption. However, the importance of cats in transmitting T. gondii infection through oocysts to humans has seldom been assessed. A total of 300 fecal samples of stray cats captured around Seoul from June to August 2013 were examined for T. gondii B1 gene (indicating the presence of oocysts) using nested-PCR. Fourteen (4.7%) of 300 cats examined were positive for B1 gene. Female cats (7.5%) showed a higher prevalence than male cats (1.4%). Cats younger than 3 months (5.5%) showed a higher prevalence than cats (1.5%) older than 3 months. For laboratory passage of the positive samples, the fecal suspension (0.2 ml) of B1 gene positive cats was orally inoculated into experimental mice. Brain tissues of the mice were obtained after 40 days and examined for the presence of tissue cysts. Two isolates were successfully passaged (designated KNIH-1 and KNIH-2) and were molecularly analyzed using the SAG5D and SAG5E gene sequences. The SAG5D and SAG5E gene sequences showed high homologies with the ME49 strain (less virulent strain). The results indicated the importance of stray cats in transmitting T. gondii to humans in Korea, as revealed by detection of B1 gene in fecal samples. T. gondii isolates from cats were successfully passaged in the laboratory for the first time in Korea.
참고문헌 (17)
- 임혜미, "Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea", The Korean Journal of Parasitology, 50(4), pp.287~293 (2012), 대한기생충학ㆍ열대의학회
- Jones JL, "Toxoplasma gondii infection in the United States: seroprevalence and risk factors", Am J Epidemiol, 154, pp.357~365 (2001)
- Sohn WM, "Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA", Korean J Parasitol, 37, pp.249~256 (1999)
- 김혜연, "Prevalence of Toxoplasma gondii in Stray Cats of Gyeonggi-do, Korea", The Korean Journal of Parasitology, 46(3), pp.199~201 (2008), 대한기생충학ㆍ열대의학회
- Lee JY, "Nested PCR-based detection of Toxoplasma gondii in German shepherd dogs and stray cats in South Korea", Res Vet Sci, 85, pp.125~127 (2008)
- 홍성희, "Prevalence of Toxoplasma gondii Infection in Household Cats in Korea and Risk Factors", The Korean Journal of Parasitology, 51(3), pp.357~361 (2013), 대한기생충학ㆍ열대의학회
- 이상은, "Prevalence of Toxoplasma gondii Infection in Stray and Household Cats in Regions of Seoul, Korea", The Korean Journal of Parasitology, 48(3), pp.267~270 (2010), 대한기생충학ㆍ열대의학회
- Lee SE, "Prevalence of Toxoplasma gondii infection in feral cats in Seoul, Korea", J Parasitol, 97, pp.153~155 (2011)
- Khan A, "Genetic analyses of atypical Toxoplasma gondii strains reveal a fourth clonal lineage in North America", Int J Parasitol, 41, pp.645~655 (2011)
- Dubey JP, "Genetic characterisation of Toxoplasma gondii in wildlife from North America revealed widespread and high prevalence of the fourth clonal type", Int J Parasitol, 41, pp.1139~1147 (2011)
- 채종일, "Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea", The Korean Journal of Parasitology, 41(3), pp.147~154 (2003), 대한기생충학ㆍ열대의학회
- Aifen Lin, "Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii", The Korean Journal of Parasitology, 43(1), pp.1~12 (2005), 대한기생충학ㆍ열대의학회
- Hong SH, "Prevalence and molecular characterizations of Toxoplasma gondii and Babesia microti from small mammals captured in Gyeonggi and Gangwon Provinces, Republic of Korea", Vet Parasitol, 205, pp.512~517 (2014)
- Jones CD, "Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor", Invest Ophthalmol Vis Sci, 41, pp.634~644 (2000)
- Tinti M, "Analysis of the SAG5 locus reveals a distinct genomic organisation in virulent and avirulent strains of Toxoplasma gondii", Int J Parasitol, 33, pp.1605~1616 (2003)
- Dumètre A, "How to detect Toxoplasma gondii oocysts in environmental samples?", FEMS Microbiol Rev, 27, pp.651~661 (2003)
- Wu SM, "Seroprevalence of Toxoplasma gondii infection in household and stray cats in Lanzhou, northwest China", Parasit Vectors, 4, pp.214~ (2011)