본문 바로가기
KERD
논문KCI 등재

Glutathione S-Transferase M1 및 T1과 N-Acetyltransferase 2다형성이 대장암 발생에 미치는 영향

Influences of Genetic Polymorphisms of Glutathione S-transferase M1 and T1,and N-acetyltransferase 2 on Colorectal Cancer Risk

김현주|이진경|황대용|홍영준|홍석일|장윤환|문선미|정소연
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2005.12|Annals of Laboratory Medicine|제25권 제6호|pp. 465-470

0

조회

0

다운로드

0

인용

0

참고문헌

주제분류병리학

초록

목적 : 발암물질의 대사에 관여하는 유전적 다형성은 대장암발생 위험도에 영향을 미친다. 본 연구에서는 한국인에서 glutathioneS-transferase M1 (GSTM1), GSTT1, N-acetyltransferase 2(NAT2)의 다형성과 대장암과의 잠재적인 관련성을 알아보고자하였다.방법 : 대장암이 판정된 환자 98명과 환자군에 성과 연령을 짝지어 무작위로 뽑은 98명의 정상 대조군을 대상으로 GSTM1,GSTT1, NAT2 다형성의 빈도에 관하여 비교하였다. multiplexpolymerase chain reaction (PCR)을 시행함으로써 GSTM1과GST1 다형성을 동시에 분석하였고, NAT2 유전자형을 분석하기 위해 real-time PCR 시스템의 melting curve 분석법을 시행하였다. GSTM1 null genotype, GSTT1 null genotype 그리고NAT2 rapid type을 risk genotype으로 간주하였다. 결과 : 대장암 환자의 58.2%와 정상 대조군의 4 4.9%에서GSTM1 null genotype을 나타냈으며(odds ratio, OR=1.706,P=0.086), 대장암 환자의 54.1%와 정상 대조군의 48.0%에서GSTT1 null genotype을 보였다(OR=1.278, P=0.475). NAT2rapid type은 대장암 환자의 51.0%와 정상 대조군의 45.9%에서나타났다(OR=1.227, P=0.568). Risk genotype의 개수가 증가할수록 대장암 발생 위험도가 증가하는 것으로 나타났다(P=0.032). 결론 : GSTM1, GSTT1, NAT2 유전자의 다형성은 개별적으로는 대장암의 위험도를 높이는데 기여하지 않았지만, GSTM1, GSTT1, NAT2 위험유전형의 개수와 대장암 위험도간에는 양-반응 관계가 성립된다고 볼 수 있다.

Abstract

Background : Inherited polymorphisms that affect carcinogen metabolism may influence the risk for the development of colorectal cancer. This study was performed to evaluate the potential association between glutathione S-transferase M1 (GSTM1), GSTT1 and N-acetyltransferase 2 (NAT2) polymorphisms and colorectal cancer in Korea. Methods : The frequencies of GSTM1, GSTT1, and NAT2 polymorphisms were compared between 98 patients with colorectal cancer and age and sex matched 98 healthy controls. GSTM1 and GSTT1 polymorphisms were simultaneously determined by multiplex polymerase chain reaction (PCR) and NAT2 polymorphisms were analyzed by real-time PCR with melting curve analysis. GSTM1 null, GSTT1 null and NAT2 rapid type were considered as risk genotypes. Results : The prevalence of GSTM1 null genotype was 44.9% in the controls, and 58.2% in the cases (odds ratio, OR=1.706, P=0.086). The prevalence of GSTT1 null genotype in the controls and the cases was 48.0% and 54.1%, respectively (OR=1.278, P=0.475). NAT2 rapid type were found in 45.9% of healthy controls and 51.0% of cancer patients (OR=1.227, P=0.568). Relative risk for colorectal cancer increased significantly (P=0.032) as the number of risk genotypes increased. Conclusions : These results suggest that genetic polymorphisms of GSTM1, GSTT1 and NAT2 have no independent effect on colorectal cancer risk individually, but there exists a dose-response relationship between a combined number of the risk genotypes of GSTM1, GSTT1 and NAT2 and colorectal cancer risk.

참고문헌 (27)

  1. "Combined influence of genetic and dietary factors oncolorectal cancer incidence in japanese Americans", 26, pp.101~5 (1999)
  2. "A pharmacogenetic study to investigate the role of dietary carcinogensin the etiology of colorectal cancer", 23, pp.1839~49 (2002)
  3. "Glutathione S-transferase polymorphisms and risk of colorectaladenomas", 163, pp.201~6 (2001)
  4. "A multiplexPCR procedure for polymorphic analysis of GSTM1 and GSTT1 genesin population studies", 107, pp.229~33 (1996)
  5. "The glutathione S-transferase supergenefamily:regulation of GST and the contribution of the isoenzyme tocancer chemoprotection and drug resistance", 30, pp.445~600 (1995)
  6. "The human glutathione S-transferase supergene family,its polymorphism,and its effects on susceptibility to lung cancer", 98, pp.87~94 (1992)
  7. "Relationshipbetween the GSTM1 genetic polymorphism and susceptibility tobladder,breast and colon cancer", 14, pp.1821~4 (1993)
  8. "Glutathione S-transferase GSTT1 genotypes and susceptibilityto cancer:studies of interactions with GSTM1 in lung,oral,gastricand colorectal cancers", 17, pp.881~4 (1996)
  9. "and susceptibility to prostate cancer in Northern India", pp.170~3
  10. "Effects of N-acetyl transferase1 and 2 polymorphisms on bladder cancer risk in Caucasians", 581, pp.97~104 (2005)
  11. "Molecular genetics and function of NAT1 and NAT2:rolein aromatic amine metabolism and carcinogenesis", 507(6), pp.65~77 (2002)
  12. "Acetylation genotype and the genetic susceptibility to prostatecancer in a southern European population", 64, pp.246~52 (2005)
  13. "NAT2 slow acetylator genotype as an important modifierof breast cancer risk", 114, pp.579~84 (2005)
  14. "Acetylationphenotype in colorectal carcinoma", 47, pp.1466~9 (1987)
  15. "Polymorphisms of glutathione-S-transferase and arylamine N-acetyltransferaseenzymes and susceptibility to colorectal cancer", 24, pp.3965~70 (2004)
  16. "Diet,acetylator phenotype,and risk of colorectal neoplasia", 347, pp.1372~4 (1996)
  17. "Ethnicdistribution of the glutathione transferase Mu 1-1(GSTM1)null genotypein 1473 individuals and application to baldder cancer susceptibility", 15, pp.1077~81 (1994)
  18. "Ethnic differences in the prevalence of the homozygous deletedgenotype of glutathione S-transferase theta", 16, pp.1243~5 (1995)
  19. "^Glutathione S-transferasepolymorphisms", pp.7~32 (2000)
  20. "Slow acetylator mutations in thehuman polymorphic N-acetyltransferase gene in 786 Asians application to metabolic epidemiology", pp.827~34 (1993)
  21. "Ethnic distribution of slow acetylatormutations in the polymorphic N-acetyltransferase(NAT-2)gene", 4, pp.125~34 (1994)
  22. "Polymorphismsin GSTP1,GSTM1,and GSTT1 and susceptibility to colorectalcancer", 8, pp.289~92 (1999)
  23. "Glutathione S-transferase GSTM1 and GSTT1 polymorphismsand colorectal cancer risk:a prospective study", 7, pp.1001~5 (1998)
  24. "Polymorphism of glutathione S-transferase lociGSTM1 and GSTT1 and susceptibility to colorectal cancer in Egypt", 142, pp.97~104 (1999)
  25. "Analysisof CYP 2E1 and GSTM1 polymorphisms for the determination ofgenetic susceptibility to Korean patients with colon cancer 한국인 대장암 환자의 유전적 감수성 결정을 위한CYP2E1 및 GSTM1 다형성 분석", pp.600 600~10 10
  26. "Glutathione S-transferase T1 and M1 genotypes innormal mucosa,transitional mucosa and colorectal adenocarcinoma", 84, pp.135~8 (1999)
  27. "Polyadenylation polymorphism in the acetyltransferase 1gene(NAT1)increases risk of colorectal cancer", 55, pp.3537~42 (1995)

관련 논문AI추천

Glutathione S-transferases (GSTM1, GSTT1 and GSTP1) and N-acetyltransferase 2 Polymorphisms and the Risk of Gastric Cancer김정완, 김호각, 류준욱, 박인규, 손윤경, 이종영, 이창형, 홍수형대한예방의학회 · 예방의학회지2006 · 제39권 제2호Association between ALDH2 and ADH1B Polymorphisms and the Risk for Colorectal Cancer in Koreans권순석, 김혜연, 신민호, 조상희, 최창균, Jirong Long, Wei Zheng대한암학회 · Cancer Research and Treatment2021 · 제53권 제3호Genetic Polymorphism of Epoxide Hydrolase and GSTM1 in Lung Cancer Susceptibility of Korean Population김영철, 박경옥, 박종태, Eun Joung Kim, Gye Jung Cho, Jin Young Ju, Jun Hwa Hwang, Kyung Hwa Park, Kyu Sik Kim, Sung Chul Lim, Sung-Ja Ahn, Yu Il Kim대한암학회 · Cancer Research and Treatment2003 · 제35권 제6호The COX-2-1195AA Genotype Is Associated with Diffuse-Type Gastric Cancer in Korea김경호, 김학양, 김형수, 신운건, 이진헌, 장명국, 조성진, Ha Jung Kim거트앤리버 소화기연관학회협의회 · Gut and Liver2012 · 제6권 제3호The diplotype of UGT1A1 and UGT1A9 genes may predict an increased risk for lung cancer강대용, 강진형, 김인경, 김정오, 최정란, Hyun Suk Chae한국보건정보통계학회 · 보건정보통계학회지2009 · 제34권 제1호한국인과 중국 연변지역의 조선족에서 hOGG과 L-myc 그리고 대사효소 등의 다형성과 흡연 및 음주가 폐암발생에 미치는 영향에 대한 비교연구김헌, 강종원, 김용대, 노성일, 엄상용, 이계영, 임동혁, 최강현, 하정호대한임상건강증진학회 · Korean Journal of Health Promotion2006 · 제6권 제2호

이 간행물의 다른 논문 (13건)

혈소판응집에 의한 위혈소판감소증의 검사실적 교정조덕, 오봉준, 양동욱, 신종희, 기승정, 서순팔, 신명근pp. 373-378인간면역결핍바이러스 감염 환자에서 발생한 급성적아구성백혈병 1예박명희, 조한익, 이동순, 이종윤pp. 385-388전립선암 진단에 있어서 Architect 총 PSA 화학발광면역검사법의 유용성 평가서소연, 조성은, 홍기숙pp. 389-393검사전 오차를 최소화하여 재설정한 혈장 음이온차의 참고범위백은정, 박일규pp. 394-398요검체에서 분리된 Providencia rettgeri 에서 VIM-2형Metallo-β-Lactamase의 생성신경섭, 김정민pp. 399-405
원문 보기