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5년간 울산대학교병원에서 HPLC법을 이용한 Mycobacteria의 동정과 균종 분포

Identification of Mycobacteria Species by HPLC and Species Distribution during Five Years atUlsan University Hospital

이선호|장철훈|김성률|정윤성
대한진단검사의학회 (Korean Society For Laboratory Medicine)|2008.02|Annals of Laboratory Medicine|제28권 제1호|pp. 24-33

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초록

Abstract

Background : Infections caused by mycobacteria have been significantly increasing. Due to the difficulty of making a decision about the pathogenicity of mycobacteria, species-level identification is very important for patients’ diagnosis and treatment. The purpose of this study was to identify mycobacteria species using a high performance liquid chromatography (HPLC) method and to provide an initial database for the distribution of mycobacteria in Korea. Methods : Acid fast bacteria isolated from 3,107 clinical specimens were identified by mycolic acid analysis using HPLC. The HPLC patterns were compared with those of standard mycobacteria species. Results : The HPLC patterns were divided into single, double, and triple cluster groups, each group comprising 9, 20, and 4 species, respectively. Mycobacteria and non-tuberculous mycobacteria (NTM) were identifies by HPLC at the rates of 99.5% and 95.6%, respectively. NTM was isolated in 12.4% of the mycobacteria positive specimens. This study also found that there were 20 different NTM species with the distribution of each species ranging from 0.3% to 15.9% of the total NTM. While the rate of NTM has been increasing in Korea, M. avium-intracellulare, M. fortuitum, and M. chelonae are relatively decreasing, and M. kansasii and M. gordonae are relatively increasing. Conclusions : HPLC method was highly discriminative for the identification of NTM in clinical specimens. (Korean J Lab Med 2008;28:24-33)

참고문헌 (40)

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관련 논문AI추천

Distribution and Clinical Significance of NontuberculousMycobacteria Identified by High Performance LiquidChromatography in Clinical Specimens김성률, 이선호, 장철훈, 정윤성대한임상미생물학회 · Annals of Clinical Microbiology2008 · 제11권 제1호The Combination of Real-Time PCR and HPLC for the Identification of Non-Tuberculous Mycobacteria김성렬, 나승원, 박재선, 서광원, 안종준, 이선호, 임지훈, 전재범, 정윤성, 정조셉, 최정인, Yangjin Jegal대한진단검사의학회 · Annals of Laboratory Medicine2013 · 제33권 제5호Denaturing HPLC를 이용한 리팜핀 내성 결핵균의 검출권필승, 김종완, 남윤형, 박수민, 안영창, 이상현, 장원철, 조민호대한진단검사의학회 · Annals of Laboratory Medicine2008 · 제28권 제2호Current status of mycobacterial identification in clinical laboratories in Korea김시현, 신정환대한임상미생물학회 · Annals of Clinical Microbiology2025 · 제28권 제3호항산성균 감염증의 신속진단박정준대한임상검사과학회 · 대한임상검사과학회지1995 · 제27권 제1호임상검체별 결핵균 검출을 위한 자동화 중합효소연쇄반응 검사의 유용성신소영, 최우순대한임상검사과학회 · 대한임상검사과학회지2006 · 제38권 제3호

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