In-House PCR법을 이용한 HLA-B27 형별 검사의 유용성 검토;PCR 검사 키트와 In-House PCR의 일치율 비교
Utility of In-House PCR for HLA-B27 Typing: Comparison of Concordance Ratebetween PCR Kit and In-House PCR
1
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Background : Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit. Methods : HLA-B27 typing was done in 188 patients by using two PCR methods, AbsoluteTM HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA). Results : The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%). Conclusions : The in-house PCR is a reliable and cost-effective method and can replace or supplement commercial kits for HLA-B27 typing. (Korean J Lab Med 2008;28:239-43)
참고문헌 (11)
- Seipp MT, "HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assay", Cytometry B Clin Cytom, 63, pp.10~15 (2005)
- Downing J, "A high-resolution polymerase chain reaction-sequence-specific primer HLA-B*27 typing set and its application in routine HLAB27 testing", Genet Test, 10, pp.98~103 (2006)
- Nathalang O, "Jaruchaimontree C. HLA-B27 testing in Thai patients using the PCR-SSP technique", Tissue Antigens, 67, pp.233~236 (2006)
- Bunce M, "Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4, DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers (PCR-SSP)", Tissue Antigens, 46, pp.355~367 (1995)
- Zino E, "Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by groupspecific polymerase chain reaction", Tissue Antigens, 63, pp.88~92 (2004)
- Lucotte G, "DNA typing of HLA-B27 by polymerase chain reaction", Mol Cell Probes, 11, pp.313~315 (1997)
- Bonnaud G, "Optimisation of HLA-B27 testing by association of flow cytometry and DNA typing", Clin Rheumatol, 18, pp.23~27 (1999)
- Lee KW, "HLA-B27 typing using PCR-SSP. Korean J Clin Pathol 1996;16:744-50. (이경화 및 김진영. PCR-SSP법을 이용한 HLAB27 검사", 대한임상병리학회지, 16, pp.744~750 (1996)
- Ji-HyunLee, "Higher Prevalence of Peripheral Arthritis Among Ankylosing Spondylitis Patients", Journal of Korean Medical Science, 17(5), pp.669~673 (2002), 대한의학회
- Bae JS, "Comparison of HLA-B27 typing methods- PCR-SSP, microlymphocytotoxicity, and flow cytometry-", Korean J Clin Pathol, 20, pp.198~203 (2000)
- Inaoui R, "Outcome of patients with undifferentiated chronic monoarthritis: retrospective study of 46 cases", Joint Bone Spine, 71, pp.209~213 (2004)