Antimicrobial Susceptibility Patterns of Legionella isolates in the Environment and in Patients
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Background : Antimicrobial susceptibility of Legionella spp. has rarely been studied in Korea. Therefore, we aimed to determine the susceptibility of Legionella spp. to various antibiotics. Methods : We assessed the antimicrobial susceptibility of 66 environmental and clinical Legionella isolates collected between January 2001 and December 2008 from Korea and Japan. The minimum inhibitory concentrations (MICs) of 6 antibiotics, namely, azithromycin, ciprofloxacin, clarithromycin,clindamycin, gatifloxacin, and gemifloxacin were determined by the broth microdilution method using buffered starch yeast extract broth. Results : The MIC ranges of the 6 antibiotics used against the Legionella isolates were as follows: 0.004-0.062 mg/mL (azithromycin), 0.002-0.5 mg/mL (ciprofloxacin), 0.004-0.5 mg/mL (clarithromycin), 0.12-4 mg/mL (clindamycin), 0.002-0.12 mg/mL (gatifloxacin), and 0.008-1 mg/mL (gemifloxacin). Conclusions : Legionella spp. isolates from Korea and Japan were most susceptible to gatifloxacin. Azithromycin, clarithromycin, ciprofloxacin, and gemifloxacin were also effective for treating legionellosis. (Korean J Lab Med 2010;30:28-33)
Abstract
Background : Antimicrobial susceptibility of Legionella spp. has rarely been studied in Korea. Therefore, we aimed to determine the susceptibility of Legionella spp. to various antibiotics. Methods : We assessed the antimicrobial susceptibility of 66 environmental and clinical Legionella isolates collected between January 2001 and December 2008 from Korea and Japan. The minimum inhibitory concentrations (MICs) of 6 antibiotics, namely, azithromycin, ciprofloxacin, clarithromycin,clindamycin, gatifloxacin, and gemifloxacin were determined by the broth microdilution method using buffered starch yeast extract broth. Results : The MIC ranges of the 6 antibiotics used against the Legionella isolates were as follows: 0.004-0.062 mg/mL (azithromycin), 0.002-0.5 mg/mL (ciprofloxacin), 0.004-0.5 mg/mL (clarithromycin), 0.12-4 mg/mL (clindamycin), 0.002-0.12 mg/mL (gatifloxacin), and 0.008-1 mg/mL (gemifloxacin). Conclusions : Legionella spp. isolates from Korea and Japan were most susceptible to gatifloxacin. Azithromycin, clarithromycin, ciprofloxacin, and gemifloxacin were also effective for treating legionellosis. (Korean J Lab Med 2010;30:28-33)
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