Cloning of a pore-forming subunit of ATP-sensitive potassium channel from Clonorchis sinensis

Abstract

A complete cDNA sequence encoding a pore-forming subunit (Kir6.2) of ATP-senstive potassiumchannel in the adult worm, Clonorchis sinensis, termed CsKir6.2, was isolated from an adult cDNA library. The cDNAcontained a single open-reading frame of 333 amino acids, which has a structural motif (a GFG-motif) of the putativepore-forming loop of the Kir6.2. Peculiarly, the CsKir6.2 shows a lack-sequence structure, which deleted 57 aminoas other known other Kir6.2 subunits. The mRNA was weekly expressed in the adult worm.