Seroprevalence of Toxocariasis among Healthy People with Eosinophilia
2
조회
0
다운로드
15
인용
0
참고문헌
키워드
초록
The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.
Abstract
The aim of this study is to determine the Toxocara seropositive rate among healthy people with eosinophilia. A total of 97 people residing in Seoul who were healthy and whose blood eosinophilia was over 10%, as shown by regular health check-ups in 2004, were subjected to this study. Their sera were tested by immunoblotting and ELISA with the antigen of larval Toxocara canis excretory-secretory (ES) protein. Sixty-five sera were band-positive (67.0%). The seropositve control sera were positive to band sizes of 66 kDa, 56 kDa, 32 kDa, and 13 kDa. In ELISA, 63 sera (65.0%) were positive to T. canis ES protein. There was no significant correlation between the IgG ELISA titer and the level of eosinophilia (r = 0.156, P = 0.156). As there were insufficient data to determine whether there were cross-reactions with other helminthic infections, or whether atopy occurred, further studies are required to verify the cause of the seropositive reactions against T. canis ES antigen. Toxocariasis seropositivity is suggested to be the major cause of eosinophilia, since the Toxocara seroprevalence among Korean rural adults was shown to be approximately 5%.
참고문헌 (15)
- Magnaval J. F., "Epidemiology of human toxocariasis in La Reunion", Transactions of the Royal Society of Tropical Medicine and Hygiene, 88, pp.531~533 (1994)
- 김용헌, "Prevalence of Toxocara canis, Toxascaris leonina and Dirofilaria immitis in dogs in Chuncheon, Korea (2004)", The Korean Journal of Parasitology, 43(2), pp.1~8 (2005), 대한기생충학회
- Hyun-YoungPARK, "A seroepidemiological survey for toxocariasis in apparently healthy residents in Gangwon-do, Korea", The Korean Journal of Parasitology, 40(3), pp.113~117 (2002), 대한기생충학회
- Malla N., "A serological study of human toxocariasis in north India", NATIONAL MEDICAL JOURNAL OF INDIA, 15, pp.145~147 (2002)
- Jernej LOGAR, "Seroprevalence of Toxocara antibodies among patients suspected of ocular toxocariasis in Slovenia (Brief Communication)", The Korean Journal of Parasitology, 42(3), pp.137~140 (2004), 대한기생충학회
- Fillaux J., "Epidemiology of toxocariasis in a steppe environment: the Patagonia study", AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 76, pp.1144~1147 (2007)
- Choi J. H., "Clinical significance of serum ECP and sero-prevalence of human toxocariasis in patients with eosinophilia", Journal of Allergy and Clinical Immunology, 23, pp.26~32 (2003)
- Kwon N. H., "The Clinical impact of toxocariasis in patients with unknown eosinophilia", Journal of Allergy and Clinical Immunology, 25, pp.299~304 (2005)
- Maizels R. M., "Shared carbohydrate epitopes on distinct surface and secreted antigens of the parasitic nematode Toxocara canis", JOURNAL OF IMMUNOLOGY, 139, pp.207~214 (1987)
- Magnaval J. F., "Application of the western blotting procedure for the immunodiagnosis of human toxocariasis", PARASITOLOGY RESEARCH, 77, pp.697~702 (1991)
- Nunes C. M., "Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique", Revista do Instituto de Medicina Tropical de Sao Paulo, 39, pp.253~256 (1997)
- R.D. Iddawela, "Characterization of a Toxocara canis species-specific excretory-secretory antigen (TcES-57) and development of a double sandwich ELISA for disgnosis of visceral larva migrans", The Korean Journal of Parasitology, 45(1), pp.19~26 (2007), 대한기생충학회
- Magnaval J. F., "Eosinophil cationic protein as a possible marker of active human Toxocara infection", Allergy, 56, pp.1096~1099 (2001)
- Magnaval J. F., "Highlights of human toxocariasis", The Korean Journal of Parasitology, 39, pp.1~11 (2001), 대한기생충학회
- Jae Hoon Lim, "Eosinophilic Infiltration in Korea: Idiopathic?", Korean Journal of Radiology, 7(1), pp.4~6 (2006), 대한영상의학회