Delayed Human Neutrophil Apoptosis by Trichomonas vaginalis Lysate
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초록
Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis,could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.
Abstract
Neutrophils play an important role in the human immune system for protection against such microorganisms as a protozoan parasite, Trichomonas vaginalis; however, the precise role of neutrophils in the pathogenesis of trichomoniasis is still unknown. Moreover, it is thought that trichomonal lysates and excretory-secretory products (ESP), as well as live T. vaginalis,could possibly interact with neutrophils in local tissues, including areas of inflammation induced by T. vaginalis in humans. The aim of this study was to investigate the influence of T. vaginalis lysate on the fate of neutrophils. We found that T. vaginalis lysate inhibits apoptosis of human neutrophils as revealed by Giemsa stain. Less altered mitochondrial membrane potential (MMP) and surface CD16 receptor expression also supported the idea that neutrophil apoptosis is delayed after T. vaginalis lysate stimulation. In contrast, ESP stimulated-neutrophils were similar in apoptotic features of untreated neutrophils. Maintained caspase-3 and myeloid cell leukemia-1 (Mcl-1) in neutrophils co-cultured with trichomonad lysate suggest that an intrinsic mitochondrial pathway of apoptosis was involved in T. vaginalis lysate-induced delayed neutrophil apoptosis; this phenomenon may contribute to local inflammation in trichomoniasis.
참고문헌 (29)
- World Health Organization, "An overview of selected curable sexually transmitted diseases", WHO Global Programme on AIDS Report, ~ (1995)
- Cwikel JG, "Sexually transmissible infections among female sex workers: an international review with an emphasis on hard-to-access populations", Sex Health, 5, pp.9~16 (2008)
- Alderete JF, "The mechanisms and molecules involved in cytoadherence and pathogenesis of Trichomonas vaginalis", Parasitol Today, 11, pp.70~74 (1995)
- Buchvald D, "Vaginal leukocyte characteristics in urogenital trichomoniasis", APMIS, 100, pp.393~400 (1992)
- Ryu JS, "Production of interleukin-8 by human neutrophils stimulated with Trichomonas vaginalis", Infect Immun, 72, pp.1326~1332 (2004)
- Trevani AS, "Extracellular acidification induces human neutrophil activation", J Immunol, 162, pp.4849~4857 (1999)
- Akul C, "Molecular control of neutrophil apoptosis", FEBS Lett, 487, pp.318~322 (2001)
- Negrotto S, "Aspirin and salicylate suppress polymorphonuclear apoptosis delay mediated by proinflammatory stimuli", J Pharmacol Exp Ther, 319, pp.972~979 (2006)
- Choi KS, "Anaplasma phagocytophilum delay of neutrophil apoptosis through the p38 mitogen-activated protein kinase signal pathway", Infect Immun, 73, pp.8209~8218 (2005)
- Weinmann P, "Delayed neutrophil apoptosis in very early rheumatoid arthritis patients is abrogated by methotrexate therapy", Clin Exp Rhematol, 25, pp.885~887 (2007)
- Catarzi S, "Apoptosis and Bax, Bcl-2, Mcl-1 expression in neutrophils of Crohn’s disease patients", Inflamm Bowel Dis, 14, pp.819~825 (2008)
- Kang JH, "Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl- 1 expression", Parasite Immunol, 28, pp.439~446 (2006)
- Song HO, "Trichomonas vaginalis: reactive oxygen species mediates caspase-3 dependent apoptosis of human neutrophils", Exp Parasitol, 118, pp.59~65 (2008)
- Crouch ML, "Trichomonas vaginalis interactions with fibronectin and laminin", Microbiology, 145, pp.2835~2843 (1999)
- Ryu JS, "Effect of iron on the virulence of Trichomonas vaginalis", J Parasitol, 87, pp.457~460 (2001)
- Diamond L, "The establishment of various trichomonads of animals and man in axenic cultures", J Parasitol, 43, pp.488~490 (1957)
- Perskvist N, "Mycobacterium tuberculosis promotes apoptosis in human neutrophils by activating caspase-3 and altering expression of Bax", Bcl-xL via an oxygen-dependent pathway, 2002, pp.168, 6358~6365
- Salvioli S, "JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis", FEBS Lett, 411, pp.77~82 (1997)
- Hart SP, "Molecular characterization of the surface of apoptotic neutrophils: implications for functional downregulation and recognition by phagocytes", Cell Death Differ, 7, pp.493~503 (2000)
- Fadeel B, "Apoptosis in human disease: a new skin for the old ceremony?", Biochem Biophys Res Commun, 266, pp.699~717 (1999)
- Lotz S, "Beta-lactam antibiotic-induced release of lipoteichoic acid from Staphylococcus aureus leads to activation of neutrophil granulocytes", Ann Clin Microbiol Antimicrob, 5, pp.1~ (2006)
- Chaible LM, "Effect of Cysticercus cellulosae on neutrophil function and death", Vet Parasitol, 127, pp.121~129 (2005)
- Sim S, "NADPH oxidase-derived reactive oxygen species-mediated activation of ERK1", 2 is required for apoptosis of human neutrophils induced by Entamoeba histolytica, 2005, pp.174, 4279~4288
- Kim JS, "Inhibition of apoptosis in human neutrophils by Helicobacter pylori water-soluble surface proteins", Scand J Gastroenterol, 36, pp.589~600 (2001)
- van Zandbergen G, "Cutting edge: neutrophil granulocyte serves as a vector for Leishmania entry into macrophages", J Immunol, 173, pp.6521~6525 (2004)
- 김지영, "Toxoplasma gondi Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation", Yonsei Medical Journal, 47(6), pp.862~869 (2006), 연세대학교의과대학
- Maianski NA, "Apoptosis of neutrophils", Acta Haematol, 111, pp.56~66 (2004)
- Edwards SW, "Regulation of neutrophil apoptosis by Mcl-1", Biochem Soc Trans, 32, pp.489~492 (2004)
- Cross A, "The dual effects of TNFalpha on neutrophil apoptosis are mediated via differential effects on expression of Mcl-1 and Bfl-1", Blood, 111, pp.878~884 (2008)