Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
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Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis.
Abstract
Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O2.-) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis.
참고문헌 (32)
- Fouts AC, "Trichomonas vaginalis : reevaluation and its clinical presentation and laboratory diagnosis", J Infect Dis, 141, pp.137~143 (1993)
- Prevalence and incidence of selected sexually transmitted infections, Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis, and Trichomonas vaginalis: methods and results used by the WHO to generate 2005 estimates, "World Health Organization", WHO, ~ (2011)
- Choi KS, "Prevalence of vaginal Trichomanas vaginalis in Kangwon area", Korean J Obstet Gynecol, 39, pp.1273~1278 (1996)
- Ryu JS, "Diagnosis of trichomoniasis by polymerase chain reaction", Yonsei Medical J, 40, pp.56~60 (1999)
- Bar Chama N, "Infection and pyospermia in male infertility", World J Urol, 11, pp.76~81 (1993)
- Jong Jin Lee, "PCR for Diagnosis of Male Trichomonas vaginalis Infection with Chronic Prostatitis and Urethritis", The Korean Journal of Parasitology, 50(2), pp.157~159 (2012), 대한기생충학ㆍ열대의학회[DOI]
- Rein MF, "Trichomonacidal activity of human polymorphonuclear neutrophils : killing by disruption and fragmentation", J Infect Dis, 142, pp.575~585 (1980)
- Davies MJ, "Myeloperoxidase-derived oxidation : mechanisms of biological damage and its prevention", J Clin Biochem Nutr, 48, pp.8~19 (2011)
- Ryu JS, "Effect of iron on the virulence of Trichomonas vaginalis", J Parasitol, 87, pp.457~460 (2001)
- Diamond LS, "The establishment of various trichomonads of animals and man in axenic cultures", J Parasitol, 43, pp.488~490 (1957)
- Min DY, "Degradation of human immunoglobulins and cytotoxicity on HeLa cells by live Trichomonas vaginalis", Korean J Parasitol, 35, pp.39~46 (1997)
- Shaio MF, "Generation of interleukin-8 from human monocytes in response to Trichomonas vaginalis stimulation", Infect Immun, 63, pp.3864~3870 (1995)
- Shin MH, "Excretory-secretory product of Paragonimus westermani newly excysted metacercariae inhibits superoxide production of granulocytes stimulated with IgG", Korean J Parasitol, 38, pp.103~106 (2000)
- Li PF, "Reactive oxygen species induce apoptosis of vascular smooth muscle cell", FEBS Lett, 404, pp.249~252 (1997)
- Schreck R, "Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1", EMBO J, 10, pp.2247~2258 (1991)
- Chaible LM, "Effect of cysticercus cellulosae on neutrophil function and death", Vet Parasitol, 127, pp.121~129 (2005)
- Leoratti FM, "Neutrophil paralysis in Plasmodium vivax malaria", PLoS Negl Trop Dis, 6, pp.e1710~ (2012)
- McQuaid KE, "Endothelial barrier dysfunction and oxidative stress : roles for nitric oxide?", Exp Physiol, 82, pp.369~376 (1997)
- Candeias LP, "Free hydroxyl radicals are formed on reaction between the neutrophil-derived species superoxide anion and hypochlorous acid", FEBS Lett, 333, pp.151~153 (1993)
- Zhu L, "Bactericidal activity of peroxynitrite", Arch Biochem Biophys, 298, pp.452~457 (1992)
- Kettle AJ, "Superoxide enhances hypochlorous acid production by stimulated human neutrophils", Biochem Biophys Acta, 1052, pp.379~385 (1990)
- Winterbourn CC, "Biological reactivity and biomarkers of the neutrophil oxidant, hypochlorous acid", Toxicology, 181 & 182, pp.223~227 (2002)
- Styrt B, "Chemorepulsion of trichomonads by products of neutrophil oxidative metabolism", J Infect Dis, 163, pp.176~179 (1991)
- Park KJ, "Leukocyte chemotaxis to Trichomonas vaginalis", Yonsei J Med Sci, 17, pp.77~87 (1984)
- Coombs GH, "An analysis of the proteinases of Trichomonas vaginalis by polyacrylamide gel electrophoresis", Parasitology, 86(pt. 1), pp.1~6 (1983)
- Arroyo R, "Trichomonas vaginalis surface proteinase activity is necessary for parasite adherence to epithelial cells", Infect Immun, 57, pp.2991~2997 (1989)
- Ryu JS, "Production of interleukin-8 by human neutrophils stimulated with Trichomonas vaginalis", Infect Immun, 72, pp.1326~1332 (2004)
- Nguyen C, "Myeloperoxidase deficiency manifesting as pustular candida dermatitis", Clin Infect Dis, 24, pp.258~260 (1997)
- Aratani Y, "Severe impairment in early host defense against Candida albicans in mice deficient in myeloperoxidase", Infect Immun, 67, pp.1828~1836 (1999)
- Pacheco-Yépez J, "Myeloperoxidase binds to and kills Entamoeba histolytica trophozoites", Parasite Immunol, 33, pp.255~264 (2011)
- Hampton MB, "Involvement of superoxide and myeloperoxidase in oxygen-dependent killing of Staphylococcus aureus by neutrophils", Infect Immun, 64, pp.3512~3517 (1996)
- Kutter D, "Consequences of total and subtotal myeloperoxidase deficiency : risk or benefit?", Acta Haematol, 104, pp.10~15 (2000)