Standardization of ABO Antibody Titer Measurement at Laboratories in Korea
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초록
Background: Measurement of the ABO antibody (Ab) titer is important in ABO-incompati- ble transplantation. However, to the best of our knowledge, no standard protocol or exter- nal survey program to measure the ABO Ab titer has been established in Korea. We inves- tigated the current status of ABO Ab titer measurements at various laboratories in Korea and the impact of the protocol provided to reduce interlaboratory variations in the meth- ods and results of ABO Ab titers. Methods: The Korean external quality assessment of blood bank laboratories sent external survey samples with a questionnaire to 68 laboratories across Korea for the measurement of ABO Ab titers in May 2012. After 6 months, a second set of survey samples were sent with a standard protocol to 53 of the previously surveyed laboratories. The protocol recom- mended incubation at room temperature only and use of the indirect antihuman globulin method for the tube test as well as and the column agglutination test (CAT). Results: Several interlaboratory variations were observed in the results, technical proce- dures, and methods selected for measurement. We found that 80.4% laboratories hoped to change their protocol to the provisional one. Additionally, CAT showed significantly lower variation among laboratories ( P =0.006) than the tube test. Conclusions: Our study provides baseline data regarding the current status of ABO Ab ti- ter measurement in Korea. The standard protocol and external survey were helpful to standardize the technical procedures and select methods for ABO Ab titer measurement.
Abstract
Background: Measurement of the ABO antibody (Ab) titer is important in ABO-incompati- ble transplantation. However, to the best of our knowledge, no standard protocol or exter- nal survey program to measure the ABO Ab titer has been established in Korea. We inves- tigated the current status of ABO Ab titer measurements at various laboratories in Korea and the impact of the protocol provided to reduce interlaboratory variations in the meth- ods and results of ABO Ab titers. Methods: The Korean external quality assessment of blood bank laboratories sent external survey samples with a questionnaire to 68 laboratories across Korea for the measurement of ABO Ab titers in May 2012. After 6 months, a second set of survey samples were sent with a standard protocol to 53 of the previously surveyed laboratories. The protocol recom- mended incubation at room temperature only and use of the indirect antihuman globulin method for the tube test as well as and the column agglutination test (CAT). Results: Several interlaboratory variations were observed in the results, technical proce- dures, and methods selected for measurement. We found that 80.4% laboratories hoped to change their protocol to the provisional one. Additionally, CAT showed significantly lower variation among laboratories ( P =0.006) than the tube test. Conclusions: Our study provides baseline data regarding the current status of ABO Ab ti- ter measurement in Korea. The standard protocol and external survey were helpful to standardize the technical procedures and select methods for ABO Ab titer measurement.
참고문헌 (12)
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