Emergence of Acinetobacter pittii Harboring New Delhi Metallo-β-Lactamase Genes in Daejeon, Korea
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Carbapenemase production has been reported worldwide in gram-negative bacteria, including Acinetobacter species. We detected carbapenemase-producing Acinetobacter pittii in clinical isolates in Daejeon, Korea. Twenty-one ertapenem-resistant A. pittii isolates screened with a disk diffusion method were characterized by using the Epsilon test, four multiplex PCR assays, and a multilocus sequence typing (MLST) scheme. A total of 21 A. pittii isolates harbored the metallo-β-lactamase (MBL) gene blaIMP-1 or blaNDM-1. Nineteen isolates containing blaIMP-1 were resistant to imipenem and meropenem, but two isolates harboring blaNDM-1 were susceptible to them. The sequence types (STs) of the two New Delhi MBL (NDM-1)-producing A. pittii isolates were ST70 and ST207, which differed from the STs (ST63, ST119, ST396, and a novel ST) of the IMP-1-producing A. pittii. This is the first report on NDM-1-producing A. pittii isolates in Korea. Our results emphasize that the study of NDM-1-producing gram-negative bacteria should involve carbapenem-susceptible as well as carbapenem-resistant isolates.
Abstract
Carbapenemase production has been reported worldwide in gram-negative bacteria, including Acinetobacter species. We detected carbapenemase-producing Acinetobacter pittii in clinical isolates in Daejeon, Korea. Twenty-one ertapenem-resistant A. pittii isolates screened with a disk diffusion method were characterized by using the Epsilon test, four multiplex PCR assays, and a multilocus sequence typing (MLST) scheme. A total of 21 A. pittii isolates harbored the metallo-β-lactamase (MBL) gene blaIMP-1 or blaNDM-1. Nineteen isolates containing blaIMP-1 were resistant to imipenem and meropenem, but two isolates harboring blaNDM-1 were susceptible to them. The sequence types (STs) of the two New Delhi MBL (NDM-1)-producing A. pittii isolates were ST70 and ST207, which differed from the STs (ST63, ST119, ST396, and a novel ST) of the IMP-1-producing A. pittii. This is the first report on NDM-1-producing A. pittii isolates in Korea. Our results emphasize that the study of NDM-1-producing gram-negative bacteria should involve carbapenem-susceptible as well as carbapenem-resistant isolates.
참고문헌 (20)
- Poirel L, "Carbapenem resistance in Acinetobacter baumannii: mechanisms and epidemiology", Clin Microbiol Infect, 12, pp.826~836 (2006)
- Poirel L, "Carbapenemases: molecular diversity and clinical consequences", Future Microbiol, 2, pp.501~512 (2007)
- Nordmann P, "Gram-negative bacteriae with resistance to carbapenems", Med Sci (Paris), 26, pp.950~959 (2010)
- Gundi VA, "Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter species", Microbiology, 155, pp.2333~2341 (2009)
- Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing: twentieth informational supplement, M100-S20", Clinical and Laboratory Standards Institute, ~ (2010)
- Ellington MJ, "Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases", J Antimicrob Chemother, 59, pp.321~322 (2007)
- Poirel L, "Multiplex PCR for detection of acquired carbapenemase genes", Diagn Microbiol Infect Dis, 70, pp.119~123 (2011)
- Woodford N, "Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp", Int J Antimicrob Agents, 27, pp.351~353 (2006)
- 구선회, "Genetic Basis of Multidrug-resistant Acinetobacter baumannii Clinical Isolates from Three University Hospitals in Chungcheong Province, Korea", Annals of Laboratory Medicine, 30(5), pp.498~506 (2010), 대한진단검사의학회
- Zhang R, "Emergence of NDM-producing non-baumannii Acinetobacter spp. isolated from China", Eur J Clin Microbiol Infect Dis, 33, pp.853~860 (2014)
- Diancourt L, "The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool", PLoS One, 5, pp.e10034~ (2010)
- Lee K, "Wide dissemination of OXA-type carbapenemases in clinical Acinetobacter spp. isolates from South Korea", Int J Antimicrob Agents, 33, pp.520~524 (2009)
- Yoo JS, "Nosocomial transmission of NDM-1-producing Escherichia coli ST101 in a Korean hospital", J Antimicrob Chemother, 68, pp.2170~2172 (2013)
- Janvier F, "Molecular characterization of blaNDM-1 in a sequence type 235 Pseudomonas aeruginosa isolate from France", Antimicrob Agents Chemother, 57, pp.3408~3411 (2013)
- Zong Z, "blaNDM-1-carrying Acinetobacter johnsonii detected in hospital sewage", J Antimicrob Chemother, 68, pp.1007~1010 (2013)
- Nordmann P, "How to detect NDM-1 producers", J Clin Microbiol, 49, pp.718~721 (2011)
- Dortet L, "Worldwide dissemination of the NDM-type carbapenemases in Gram-negative bacteria", Biomed Res Int, 2014, pp.249856~ (2014)
- Yang J, "Dissemination and characterization of NDM-1-producing Acinetobacter pittii in an intensive care unit in China", Clin Microbiol Infect, 18, pp.E506~513 (2012)
- Pasteran F, "Emergence of genetically unrelated NDM-1-producing Acinetobacter pittii strains in Paraguay", J Antimicrob Chemother, 69, pp.2575~2578 (2014)
- Yamamoto M, "Interspecies dissemination of a novel class 1 integron carrying blaIMP-19 among Acinetobacter species in Japan", J Antimicrob Chemother, 66, pp.2480~2483 (2011)