Prevalence of Complement-Mediated Cell Lysis-like Gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis Isolates From Japan (2014–2016)
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Background: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. Methods: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data.; they were obtained from 25 sterile (blood, joint fluid, and cerebrospinal fluid) and 30 non-sterile (skin-, respiratory tract-, and genitourinary tract-origin) samples. emm genotyping, multilocus sequence typing, sicG amplification/sequencing, and random amplified polymorphic DNA (RAPD) analysis of sicG-positive strains were performed. Results: sicG was detected in 30.9% of the isolates (16 human and one canine) and the genes from the 16 human samples (blood, 10; open pus, 3; sputum, 2; throat swab, 1) and one canine sample (open pus) showed the same sequence pattern. All sicG-harboring isolates belonged to clonal complex (CC) 17, and the most prevalent emm type was stG6792 (82.4%). There was a significant association between sicG presence and the development of skin/soft tissue infections. CC17 isolates with sicG could be divided into three subtypes by RAPD analysis. Conclusions: CC17 SDSE harboring sicG might have spread into three closely-related prefectures in central Japan during 2014–2016. Clonal analysis of isolates from other areas might be needed to monitor potentially virulent strains in humans and animals.
Abstract
Background: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. Methods: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data.; they were obtained from 25 sterile (blood, joint fluid, and cerebrospinal fluid) and 30 non-sterile (skin-, respiratory tract-, and genitourinary tract-origin) samples. emm genotyping, multilocus sequence typing, sicG amplification/sequencing, and random amplified polymorphic DNA (RAPD) analysis of sicG-positive strains were performed. Results: sicG was detected in 30.9% of the isolates (16 human and one canine) and the genes from the 16 human samples (blood, 10; open pus, 3; sputum, 2; throat swab, 1) and one canine sample (open pus) showed the same sequence pattern. All sicG-harboring isolates belonged to clonal complex (CC) 17, and the most prevalent emm type was stG6792 (82.4%). There was a significant association between sicG presence and the development of skin/soft tissue infections. CC17 isolates with sicG could be divided into three subtypes by RAPD analysis. Conclusions: CC17 SDSE harboring sicG might have spread into three closely-related prefectures in central Japan during 2014–2016. Clonal analysis of isolates from other areas might be needed to monitor potentially virulent strains in humans and animals.
참고문헌 (26)
- Vandamme P, "Taxonomic study of lancefield streptococcal groups C, G, and L (Streptococcus dysgalactiae) and proposal of S. dysgalactiae subsp. equisimilis subsp. nov", Int J Syst Bacteriol, 46, pp.774~781 (1996)
- Broyles LN, "Population-based study of invasive disease due to β-hemolytic streptococci of groups other than A and B", Clin Infect Dis, 48, pp.706~712 (2009)
- Cohen-Poradosu R, "Group G streptococcal bacteremia in Jerusalem", Emerg Infect Dis, 10, pp.1455~1460 (2004)
- Liao CH, "Bacteremia caused by group G streptococci, Taiwan", Emerg Infect Dis, 14, pp.837~840 (2008)
- Sylvetsky N, "Bacteremia due to beta-hemolytic Streptococcus group G: increasing incidence and clinical characteristics of patients", Am J Med, 112, pp.622~626 (2002)
- Takahashi T, "Invasive infection caused by Streptococcus dysgalactiae subsp. equisimilis: characteristics of strains and clinical features", J Infect Chemother, 17, pp.1~10 (2011)
- Yamaoka S, "Neonatal streptococcal toxic shock syndrome caused by Streptococcus dysgalactiae subsp. equisimilis", Pediatr Infect Dis J, 29, pp.979~981 (2010)
- Bramhachari PV, "Disease burden due to Streptococcus dysgalactiae subsp. equisimilis (group G and C streptococcus) is higher than that due to Streptococcus pyogenes among Mumbai school children", J Med Microbiol, 59, pp.220~223 (2010)
- Takahashi T, "Clinical aspects of invasive infections with Streptococcus dysgalactiae ssp. equisimilis in Japan: differences with respect to Streptococcus pyogenes and Streptococcus agalactiae infections", Clin Microbiol Infect, 16, pp.1097~1103 (2010)
- Åkesson P, "Protein SIC, a novel extracellular protein of Streptococcus pyogenes interfering with complement function", J Biol Chem, 271, pp.1081~1088 (1996)
- Fernie-King BA, "Streptococcal inhibitor of complement (SIC) inhibits the membrane attack complex by preventing uptake of C567 onto cell membranes", Immunology, 103, pp.390~398 (2001)
- Minami M, "Prevalence of a streptococcal inhibitor of a complement-mediated cell lysis-like gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis", Curr Microbiol, 62, pp.884~887 (2011)
- Smyth D, "DrsG from Streptococcus dysgalactiae subsp. equisimilis inhibits the antimicrobial peptide LL-37", Infect Immun, 82, pp.2337~2344 (2014)
- Fujita T, "Genetic diversity in Streptococcus dysgalactiae subsp. equisimilis isolates from patients with invasive and noninvasive infections in a Japanese university hospital (2014-2015)", Jpn J Infect Dis, 70, pp.100~104 (2017)
- "Streptococcus dysgalactiae subsp. equisimilis"[URL]
- Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing. Twenty-second Informational supplement, M100-S22", Clinical and Laboratory Standards Institute, ~ (2012)
- McMillan DJ, "Population genetics of Streptococcus dysgalactiae subspecies equisimilis reveals widely dispersed clones and extensive recombination", PLoS One, 5, pp.e11741~ (2010)
- Bert F, "Analysis of genetic relationships among strains of groups A, C and G streptococci by random amplified polymorphic DNA analysis", J Med Microbiol, 45, pp.278~284 (1996)
- Haenni M, "Diversity and mobility of integrative and conjugative elements in bovine isolates of Streptococcus agalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis", Appl Environ Microbiol, 76, pp.7957~7965 (2010)
- Malhotra-Kumar S, "Multiplex PCR for simultaneous detection of macrolide and tetracycline resistance determinants in streptococci", Antimicrob Agents Chemother, 49, pp.4798~4800 (2005)
- Lo HH, "Distribution of virulence factors and association with emm polymorphism or isolation site among beta-hemolytic group G Streptococcus dysgalactiae subspecies equisimilis", APMIS, 123, pp.45~52 (2015)
- Oppegaard O, "Sequence diversity of sicG among group C and G Streptococcus dysgalactiae subspecies equisimilis isolates associated with human infections in western Norway", Eur J Clin Microbiol Infect Dis, 33, pp.273~277 (2014)
- Davies MR, "Virulence profiling of Streptococcus dysgalactiae subspecies equisimilis isolated from infected humans reveals 2 distinct genetic lineages that do not segregate with their phenotypes or propensity to cause diseases", Clin Infect Dis, 44, pp.1442~1454 (2007)
- Mejia LM, "Characterization of group A Streptococcus strains recovered from Mexican children with pharyngitis by automated DNA sequencing of virulence-related genes: unexpectedly large variation in the gene (sic) encoding a complement-inhibiting protein", J Clin Microbiol, 35, pp.3220~3224 (1997)
- Schrieber L, "Transmission of Streptococcus dysgalactiae subsp. equisimilis between child and dog in an Aboriginal Australian community", Zoonoses Public Health, 61, pp.145~148 (2014)
- "One Health"[URL]