Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia
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Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.
Abstract
Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.
참고문헌 (27)
- Honigberg BM., "Trichomonads Parasitic in Humans", pp.342~393 (1990), Springer-Verlag
- Meloni D, "Molecular identification of Pentatrichomonas hominis in two patients with gastrointestinal symptoms", J Clin Pathol, 64, pp.933~935 (2011)
- Duboucher C, "Recent advances in pulmonary trichomonosis", Trends Parasitol, 24, pp.201~202 (2008)
- "Trichomonas vaginalis, protozoa pathogen saluran urogenital" (2006)[URL]
- Rakoff AE, "Results of intra-peritoneal injections of laboratory animals with various trichomonad flagellates(protozoa)", Am J Epidemiol, 19, pp.502~513 (1934)
- Tanabe M, "A new culture medium for Entamoeba histolytica", Acta Med Keijo, 11, pp.1~4 (1928)
- Duboucher C, "Molecular identification of Tritrichomonas foetus-like organisms as co-infecting agents of human Pneumocystis pneumonia", J Clin Microbiol, 44, pp.1165~1168 (2006)
- Tamura K, "MEGA5 : Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol Biol Evol, 28, pp.2731~2739 (2011)
- Smejkalová P, "Extensive diversity of intestinal trichomonads of non-human primates", Parasitology, 139, pp.92~102 (2012)
- Mattern CF, "Structure of Hypotrichomonas acosta(Moskowitz)(Monocercomonadidae, Trichomonadida)as revealed by electron microscopy", J Protozool, 16, pp.668~685 (1969)
- Diamond LS, "In vitro cultivation of the Trichomonadidae : a state of the art review", Acta Univ Carol Biol, 30, pp.221~228 (1986)
- Clark CG, "Methods for cultivation of luminal parasitic protists of clinical importance", Clin Microbiol Rev, 15, pp.329~341 (2002)
- Cepicka I, "Critical taxonomic revision of parabasalids with description of one new genus and three new species", Protist, 161, pp.400~433 (2010)
- Lollis L, "Molecular characterization of Histomonas meleagridis and other parabasalids in the United States using the 5.8S, ITS1, and ITS2 rRNA regions", J Parasitol, 97, pp.610~615 (2011)
- Hampl V, "Critical analysis of the topology and rooting of the parabasalian 16S rRNA tree", Mol Phylogenet Evol, 32, pp.711~723 (2004)
- Hampl V, "Morphological and molecular diversity of the monocercomonadid genera Monocercomonas, Hexamastix, and Honigbergiella gen. nov", Protist, 158, pp.365~383 (2007)
- Kirby H, "Flagellates of the Caecum of Ground Squirrels", pp.315~366 (1949), University of California
- Wenrich DH, "Culture experiments on intestinal flagellates;trichomonad and other flagellates obtained from man and certain rodents", J Parasitol, 32, pp.40~53 (1946)
- Nie D, "Morphology and taxonomy of the intestinal protozoa of the guinea-pig, Cavia porcella", J Morphol, 86, pp.381~494 (1950)
- Cepicka I, "New evolutionary lineages, unexpected diversity, and host specificity in the parabasalid genus Tetratrichomonas", Mol Phylogenet Evol, 39, pp.542~551 (2006)
- Walker RL, "Comparison of the 5.8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity", J Vet Diagn Invest, 15, pp.14~20 (2003)
- Crespo R, "Salpingitis in Pekin ducks associated with concurrent infection with Tetratrichomonas sp. and Escherichia coli", J Vet Diagn Invest, 13, pp.240~245 (2001)
- Gookin JL, "Molecular characterization of trichomonads from feces of dogs with diarrhea", J Parasitol, 91, pp.939~943 (2005)
- Dimasuay KGB, "Molecular characterization of trichomonads isolated from animal hosts in the Philippines", Vet Parasitol, 196, pp.289~295 (2013)
- Wenrich DH, "Morphology of the intestinal trichomonad flagellates in man and of similar forms in monkeys, cats, dogs, and rats", J Morphol, 74, pp.189~211 (1947)
- Reinmann K, "Tritrichomonas foetus isolates from cats and cattle show minor genetic differences in unrelated loci ITS-2 and EF-1α", Vet Parasitol, 185, pp.138~144 (2012)
- Felleisen RS, "Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa", Parasitology, 115, pp.111~119 (1997)