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Subtype Distribution of Blastocystis in Thai-Myanmar Border, Thailand

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초록

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

Abstract

Blastocystis sp. is a common zoonotic intestinal protozoa which has been classified into 17 subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distribution of Blastocystis in villagers living on the Thai-Myanmar border, where the risk of parasitic infection is high. A total of 207 stool samples were collected and DNA was extracted. PCR and sequencing using primers targeting small-subunit ribosomal RNA (SSU rRNA) gene were performed. The prevalence of Blastocystis infection was 37.2% (77/207). ST3 (19.8%; 41/207) was the predominant subtype, followed by ST1 (11.6%; 24/207), ST2 (5.3%; 11/207), and ST4 (0.5%; 1/207). A phylogenetic tree was reconstructed using the maximum likelihood (ML) method based on the Hasegawa-Kishino-Yano + G + I model. The percentage of bootstrapped trees in which the associated taxa clustered together was relatively high. Some sequences of Blastocystis positive samples (TK18, 39, 46, 71, and 90) were closely related to animals (pig and cattle) indicating zoonotic risks. Therefore, proper health education in parasitic prevention for the villagers should be promoted to improve their personal hygiene. Further longitudinal studies are required to monitor the prevalence of parasitic infections after providing health education and to investigate Blastocystis ST in animals living in these villages.

참고문헌 (37)

  1. Tan KS, "New insights on classification, identification, and clinical relevance of Blastocystis spp", Clin Microbiol Rev, 21, pp.639~665 (2008)
  2. Yakoob J, "Irritable bowel syndrome: is it associated with genotypes of Blastocystis hominis", Parasitol Res, 106, pp.1033~1038 (2010)
  3. Wong KH, "Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore", Parasitol Res, 102, pp.663~670 (2008)
  4. Alfellani MA, "Genetic diversity of Blastocystis in livestock and zoo animals", Protist, 164, pp.497~509 (2013)
  5. Rene BA, "Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients", Am J Trop Med Hyg, 80, pp.588~592 (2009)
  6. Souppart L, "Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt", Parasitol Res, 106, pp.505~511 (2010)
  7. Ozyurt M, "Molecular epidemiology of Blastocystis infections in Turkey", Parasitol Int, 57, pp.300~306 (2008)
  8. Roberts T, "Subtype distribution of Blastocystis isolates identified in a Sydney population and pathogenic potential of Blastocystis", Eur J Clin Microbiol Infect Dis, 32, pp.335~343 (2013)
  9. Stensvold CR, "Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study", Diagn Microbiol Infect Dis, 59, pp.303~307 (2007)
  10. Leelayoova S, "In-vitro cultivation: a sensitive method for detecting of Blastocystis hominis", Ann Trop Med Parasitol, 96, pp.803~807 (2002)
  11. Termmathurapoj S, "The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens", Parasitol Res, 93, pp.445~447 (2004)
  12. Roberts T, "Comparison of microscopy, culture, and conventional polymerase chain reaction for detection of Blastocystis sp. in clinical stool samples", Am J Trop Med Hyg, 84, pp.308~312 (2011)
  13. Clark CG, "Extensive genetic diversity in Blastocystis hominis", Mol Biochem Parasitol, 87, pp.79~83 (1997)
  14. Larkin MA, "Clustal W and Clustal X version 2.0", Bioinformatics, 23, pp.2947~2948 (2007)
  15. Tamura K, "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol Biol Evol, 28, pp.2731~2739 (2011)
  16. Hirata T, "Prevalence of Blastocystis hominis and Strongyloides stercoralis infection in Okinawa, Japan", Parasitol Res, 101, pp.1717~1719 (2007)
  17. Horiki N, "Epidemiologic survey of Blastocystis hominis infection in Japan", Am J Trop Med Hyg, 56, pp.370~374 (1997)
  18. Saksirisampant W, "Intestinal parasitic infestations among children in an orphanage in Pathum Thani Province", J Med Assoc Thai, 86, pp.S263~S270 (2003)
  19. Taamasri P, "Prevalence of Blastocystis hominis carriage in Thaiarmy personnel based in Chonburi, Thailand", Mil Med, 167, pp.643~646 (2002)
  20. Mungthin M, "Asymptomatic intestinal microsporidiosis in Thai orphans and child-care workers", Trans R Soc Trop Med Hyg, 95, pp.304~306 (2001)
  21. Meloni D, "Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy", Parasitol Res, 109, pp.613~619 (2011)
  22. Yoshikawa H, "Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries", Parasitol Res, 92, pp.22~29 (2004)
  23. Thathaisong U, "Identification of Blastocystis subtype 1 variants in the Home for Girls, Bangkok, Thailand", Am J Trop Med Hyg, 88, pp.352~358 (2013)
  24. Leelayoova S, "Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand", Am J Trop Med Hyg, 79, pp.401~406 (2008)
  25. Thathaisong U, "Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis", J Clin Microbiol, 41, pp.967~975 (2003)
  26. Parkar U, "Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers", Vet Parasitol, 169, pp.8~17 (2010)
  27. Yoshikawa H, "Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal", Vet Parasitol, 160, pp.295~300 (2009)
  28. Abe N, "Molecular and phylogenetic analysis of Blastocystis isolates from various hosts", Vet Parasitol, 120, pp.235~242 (2004)
  29. Silberman JD, "Human parasite finds taxonomic home", Nature, 380, pp.398~ (1996)
  30. Rivera WL, "Phylogenetic analysis of Blastocystis isolates from animal and human hosts in the Philippines", Vet Parasitol, 156, pp.178~182 (2008)
  31. Arisue N, "Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates", Parasitology, 126, pp.1~9 (2003)
  32. Stensvold CR, "Investigation of the molecular epidemiology of Blastocystis by use of a multi-locus sequence typing system for subtype 3 (personal communication)"
  33. Stensvold CR, "Levels of genetic diversity vary dramatically between Blastocystis subtypes", Infect Genet Evol, 12, pp.263~273 (2012)
  34. Yan MY, "Phylogenetic analysis of Blastocystis hominis isolates in China (personal communication)"
  35. Noël C, "Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences", Mol Biochem Parasitol, 126, pp.119~123 (2003)
  36. Engsbro AL, "Blastocystis: to treat or not to treat...but how?", Clin Infect Dis, 55, pp.1431~1432 (2012)
  37. Yoshikawa H, "Phylogenetic studies of Blastocystis isolates from zoo marsupial mammals inferred from the small subunit ribosomal RNA gene sequences (personal communication)"

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