Relationship Between Rotavirus P[6] Infection in Korean Neonates and Histo-Blood Group Antigen: a Single-Center Study
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Rotaviruses are a major cause of pediatric gastroenteritis. The rotavirus P[6] genotype is the most prevalent genotype isolated from Korean neonates but has rarely been reported in other countries. Histo-blood group antigen (HBGA) is known to play an important role in rotavirus infection. We investigated the relationship between rotavirus genotype and HBGA-Lewis blood type in Korean children and explored the reasons for the predominance of rotavirus P[6] strain in Korean neonates. Methods: Blood and stool samples were collected from 16 rotavirus-infected patients. Rotavirus G (VP7) and P (VP4) genotyping was performed using reverse transcription-PCR and sequencing. Lewis antigen phenotypes (Lea/Leb) were tested, and HBGA-Lewis genotype was determined by sequencing the secretor (FUT2) and Lewis (FUT3) genes. Deduced amino acid sequences and three-dimensional structures of the VP8* portion of the rotavirus VP4 protein were analyzed. Results: All P[6] rotaviruses were isolated from neonates under one month of age, who were negative or weakly positive for the Leb antigen. However, 10 of the 11 non-P[6] rotaviruses were isolated from older children who were Leb antigen-positive. The VP8* amino acid sequences differed among P[6], P[4], and P[8] genotypes. Korean P[6] strains showed a unique VP8* sequence with amino acid substitutions, including Y169>L169, which differed from the sequences of P[6] strains from other countries. Conclusions: The predominance of the rotavirus P[6] genotype in Korean neonates may be related to the interaction between HBGA-Lewis antigen and the VP8* portion of the VP4 protein, and this information will be helpful in future neonatal vaccine development.
Abstract
Background: Rotaviruses are a major cause of pediatric gastroenteritis. The rotavirus P[6] genotype is the most prevalent genotype isolated from Korean neonates but has rarely been reported in other countries. Histo-blood group antigen (HBGA) is known to play an important role in rotavirus infection. We investigated the relationship between rotavirus genotype and HBGA-Lewis blood type in Korean children and explored the reasons for the predominance of rotavirus P[6] strain in Korean neonates. Methods: Blood and stool samples were collected from 16 rotavirus-infected patients. Rotavirus G (VP7) and P (VP4) genotyping was performed using reverse transcription-PCR and sequencing. Lewis antigen phenotypes (Lea/Leb) were tested, and HBGA-Lewis genotype was determined by sequencing the secretor (FUT2) and Lewis (FUT3) genes. Deduced amino acid sequences and three-dimensional structures of the VP8* portion of the rotavirus VP4 protein were analyzed. Results: All P[6] rotaviruses were isolated from neonates under one month of age, who were negative or weakly positive for the Leb antigen. However, 10 of the 11 non-P[6] rotaviruses were isolated from older children who were Leb antigen-positive. The VP8* amino acid sequences differed among P[6], P[4], and P[8] genotypes. Korean P[6] strains showed a unique VP8* sequence with amino acid substitutions, including Y169>L169, which differed from the sequences of P[6] strains from other countries. Conclusions: The predominance of the rotavirus P[6] genotype in Korean neonates may be related to the interaction between HBGA-Lewis antigen and the VP8* portion of the VP4 protein, and this information will be helpful in future neonatal vaccine development.
참고문헌 (28)
- Desselberger U, "Rotaviruses", Virus Res, 190, pp.75~96 (2014)
- "Rotavirus Classification Working Group: RCWG–Laboratory of Viral Metagenomics"[URL]
- THANVAN THAI, "Prevalence of rotavirus genotypes in South Korea in 1989–2009: implications for a nationwide rotavirus vaccine program", Clinical and Experimental Pediatrics, 56(11), pp.465~473 (2013), 대한소아청소년과학회
- Kawai K, "Burden of rotavirus gastroenteritis and distribution of rotavirus strains in Asia: a systematic review", Vaccine, 30, pp.1244~1254 (2012)
- genotype after the introduction of rotavirus vaccines, "Vaccine", 2014, 6396~6402
- Genotypes among Neonates in Two Korean Hospitals, "Annals of Clinical Microbiology", 2017, 63~66, pp.대한임상미생물학회
- genotypes, "Gut Pathog", 2019, 37~
- rotavirus strains in Korean neonates, "Gut Pathog", 2018, 27~
- Gozalbo-Rovira R, "Unraveling the role of the secretor antigen in human rotavirus attachment to histo-blood group antigens", PLoS Pathog, 15, pp.e1007865~ (2019)
- Hu L, "Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen", Nature, 485, pp.256~259 (2012)
- Hu L, "Glycan recognition in globally dominant human rotaviruses", Nat Commun, 9, pp.2631~ (2018)
- Hong YJ, "Significance of Lewis phenotyping using saliva and gastric tissue: comparison with the Lewis phenotype inferred from Lewis and secretor genotypes", Biomed Res Int, 2014, pp.573652~ (2014)
- 김재석, "Performance Evaluation of the Automated Fluorescent Immunoassay System Rotavirus Assay in Clinical Samples", Annals of Laboratory Medicine, 39(1), pp.50~57 (2019), 대한진단검사의학회[DOI]
- 송서영, "직접염기서열분석법을 이용한 한국인 Lewis 혈액형군의 유전자분석", Blood Research, 43(1), pp.34~42 (2008), 대한혈액학회
- Serpa J, "Lewis enzyme (alpha1-3", 4 fucosyltransferase) polymorphisms do not explain the Lewis phenotype in the gastric mucosa of a Portuguese population, 2003, pp.48, 183~189
- Boniface K, "Human neonatal rotavirus vaccine (RV3-BB) produces vaccine take irrespective of histo-blood group antigen status", J Infect Dis, 221, pp.1070~1078 (2020)
- King JR, "Fucosyltransferase gene polymorphisms and Lewisb-negative status are frequent in Swedish newborns, with implications for infectious disease susceptibility and personalized medicine", J Pediatric Infect Dis Soc, 8, pp.507~518 (2019)
- Kumar S, "MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol Biol Evol, 33, pp.1870~1874 (2016)
- Waterhouse A, "SWISS-MODEL: homology modelling of protein structures and complexes", Nucleic Acids Res, 46, pp.W296~W303 (2018)
- Burley SK, "RCSB Protein Data Bank: biological macromolecular structures enabling research and education in fundamental biology, biomedicine, biotechnology and energy", Nucleic Acids Res, 47, pp.D464~D474 (2019)
- Pettersen EF, "UCSF Chimera–a visualization system for exploratory research and analysis", J Comput Chem, 25, pp.1605~1612 (2004)
- Nordgren J, "Both Lewis and secretor status mediate susceptibility to rotavirus infections in a rotavirus genotype-dependent manner", Clin Infect Dis, 59, pp.1567~1573 (2014)
- Van Trang N, "Association between norovirus and rotavirus infection and histo-blood group antigen types in Vietnamese children", J Clin Microbiol, 52, pp.1366~1374 (2014)
- Ameno S, "Lewis and Secretor gene effects on Lewis antigen and postnatal development of Lewis blood type", Biol Neonate, 79, pp.91~96 (2001)
- Todd S, "Rotavirus strain types circulating in Africa: review of studies published during 1997-2006", J Infect Dis, 202(S1), pp.S34~S42 (2010)
- asymptomatic and symptomatic neonatal rotavirus strains differ in their NSP4, VP8*, and VP7 genes, "J Med Virol", 2000, 208~216
- Rotaviruses circulating worldwide, "J Infect Dis", 2016, 1039~1049
- 박형두, "The Relationship between Lewis", Secretor Genotypes and Serum Carbohydrate Antigen 19-9 Levels in a Korean Population, 2010, pp.30(1), 51~57[DOI]