Identification of Cystoisospora ohioensis in a Diarrheal Dog in Korea
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.
Abstract
A 3-month-old female Maltese puppy was hospitalized with persistent diarrhea in a local veterinary clinic. Blood chemistry and hematology profile were analyzed and fecal smear was examined. Diarrheal stools were examined in a diagnostic laboratory, using multiplex real-time polymerase chain reaction (PCR) against 23 diarrheal pathogens. Sequence analysis was performed using nested PCR amplicon of 18S ribosomal RNA. Coccidian oocysts were identified in the fecal smear. Although multiplex real-time PCR was positive for Cyclospora cayetanensis, the final diagnosis was Cystoisospora ohioensis infection, confirmed by phylogenetic analysis of 18S rRNA. To our knowledge, this the first case report of C. ohioensis in Korea, using microscopic examination and phylogenetic analysis.
참고문헌 (10)
- Hechenbleikner EM, "Parasitic colitis", Clin Colon Rectal Surg, 28, pp.79~86 (2015)[DOI]
- Chacin-Bonilla L, "Epidemiology of Cyclospora cayetanensis: a review focusing in endemic areas", Acta Trop, 115, pp.181~193 (2010)[DOI]
- Buehl IE, "Canine isosporosis - epidemiology of field and experimental infections", J Vet Med B Infect Dis Vet Public Health, 53, pp.482~487 (2006)[DOI]
- He P, "Cystoisospora spp. from dogs in China and phylogenetic analysis of its 18S and ITS1 gene", Vet Parasitol, 190, pp.254~258 (2012)[DOI]
- Matsubayashi M, "Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan", Vet Parasitol, 176, pp.270~274 (2011)[DOI]
- Baek BK, "Studies on isosporosis in dogs. I: isolation and sporulation of Isospora ohioensis", Korean J Parasitol, 31, pp.201~206 (1993)[DOI]
- Sulaiman IM, "Genetic characterization of human-pathogenic Cyclospora cayetanensis parasites from three endemic regions at the 18S ribosomal RNA locus", Infect Genet Evol, 22, pp.229~234 (2014)[DOI]
- Taniuchi M, "Multiplex polymerase chain reaction method to detect Cyclospora, Cystoisospora, and Microsporidia in stool samples", Diagn Microbiol Infect Dis, 71, pp.386~390 (2011)[DOI]
- 2.0.CO;2,
- Sanchez-Vega JT1, "Cyclospora cayetanensis: this emerging protozoan pathogen in Mexico", Am J Trop Med Hyg, 90, pp.351~353 (2014)[DOI]