Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1
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A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclo- nal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in en- demic areas and on the globe.
Abstract
A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclo- nal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in en- demic areas and on the globe.
참고문헌 (22)
- Jentes ES, "The revised global yellow fever risk map and recommendations for vaccination, 2010 : consensus of the Informal WHO Working Group on Geographic Risk for Yellow Fever", The Lancet Infect Dis, 11, pp.622~632 (2011)
- Tomlinson W, "Centennial year of yellow fever eradication in New Orleans and the United States, 1905–2005", J La State Med Soc, 157, pp.216~217 (2005)
- Kubota RL, "Sweeping method to scan breeding places for dengue and urban yellow fever vetors", Rev Saude Publica, 37, pp.263~265 (2003)[DOI]
- Gubler D, "The changing epidemiology of yellow fever and dengue, 1900 to 2003 : full circle", Comp Immunol and Microbiol of Infect Dis, 27, pp.319~330 (2004)
- "Yellow fever" (2016)[URL]
- Morlais, "Intraspecific DNA variation in nuclear genes of mosquito Aedes aegypti", Insect Mol Biol, 12, pp.631~639 (2003)
- Ravel S, "Microsatellite markers for population genetic studies in Aedes aegypti(Diptera : Culicidae)from Cote d’Ivoire : evidence for a microgeographic genetic differentiation of mosquitoes from Bouake", Acta Trop, 82, pp.39~49 (2002)
- Vasconcelos PF, "Yellow Fever Remains a Potential Threat to Public Health", Vector Borne Zoonotic Dis, 16, pp.566~567 (2016)
- Garske T, "Yellow Fever in Africa : Estimating the Burden of Disease and Impact of Mass Vaccination from Outbreak and Serological Data", PLoS Med, 11, pp.e1001638~ (2014)
- Woodall JP, "Why is the yellow fever outbreak in Angola a ‘threat to the entire world’", Int J Infect Dis, 48, pp.96~97 (2016)
- "COES – Febre Amarela. INFORME – N° 30/2017" (2017)[URL]
- Muller DA, "The flavivirus NS1 protein : Molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker", Antiviral Res, 98, pp.192~208 (2013)
- McBride WJ, "Evaluation of dengue NS1 test kits for the diagnosis of dengue fever", Diagn Microbiol Infect Dis, 64, pp.31~36 (2009)
- Richman DD, "Rapid viral diagnosis", Infect Dis, 149, pp.298~310 (1984)
- Chernesky MA, "Detection of viral antigens, particles, and early antibodies in diagnosis", Yale J Biol Med, 57, pp.757~776 (1984)
- Lee J, "Development and clinical evaluation of a highly accurate dengue NS1 rapid test : from the preparation of a soluble NS1 antigen to the construction of an RDT", Diagn Microbiol Infect Dis, 82, pp.128~134 (2015)
- 김영훈, "Development of a Rapid Diagnostic Test Kit to Detect IgG", IgM Antibody against Zika Virus Using Monoclonal Antibodies to the Envelope and Non-structural Protein 1 of the Virus, 2018, pp.56(1), 61~70[DOI]
- Friquet B, "Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay", J Immunol Methods, 77, pp.305~319 (1985)
- 정점규, "Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen", Parasites, Hosts and Diseases, 49(3), pp.207~212 (2011), 대한기생충학ㆍ열대의학회
- Alcon S, "Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections", J Clin Microbiol, 40, pp.376~381 (2002)
- Watterson D, "The many faces of the flavivirus NS1 protein offer a multitude of options for inhibitor design", Antiviral Res, 130, pp.7~18 (2016)
- Alcalá AC, "The dengue virus non-structural protein 1(NS1)is secreted efficiently from infected mosquito cells", Virology, 488, pp.278~287 (2016)