Evaluation of a Newly Developed Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect IgG and IgM Antibodies to Chikungunya Virus
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The ichroma Chikungunya virus (CHIKV) IgG/IgM (Boditech Med Inc., Chuncheon, Korea) is a newly developed rapid lateral flow immunoassay for detection of anti- CHIKV-IgG/IgM. This study conducted with thirty-six anti-CHIKV IgG positive sera, 57 anti-CHIKV IgM positive sera and 163 anti-CHIKV IgG/IgM negative sera which were confirmed by commercial enzyme-linked immunosorbent assays (ELISAs) (Inbios CHIKjj Detect™ IgM Capture ELISA, Inbios CHIKjj Detect™ IgG ELISA (InBios International Inc., Seattle, WA, USA), Anti-CHIKV ELISA (IgM), Anti- CHIKV ELISA (IgG) (Euroimmun, Lübeck, Germany)). The ichroma detected all 36 anti-CHIKV IgG and 57 anti-CHIKV IgM positivity (100% sensitivity). For 163 anti-CHIKV IgG/IgM negative sera, the ichroma showed one false positive for IgM (99.4% specificity). The ichroma showed no cross-reactivity and no interference. The ichroma demonstrated good diagnostic performance compared to the current ELISAs.
Abstract
The ichroma Chikungunya virus (CHIKV) IgG/IgM (Boditech Med Inc., Chuncheon, Korea) is a newly developed rapid lateral flow immunoassay for detection of anti- CHIKV-IgG/IgM. This study conducted with thirty-six anti-CHIKV IgG positive sera, 57 anti-CHIKV IgM positive sera and 163 anti-CHIKV IgG/IgM negative sera which were confirmed by commercial enzyme-linked immunosorbent assays (ELISAs) (Inbios CHIKjj Detect™ IgM Capture ELISA, Inbios CHIKjj Detect™ IgG ELISA (InBios International Inc., Seattle, WA, USA), Anti-CHIKV ELISA (IgM), Anti- CHIKV ELISA (IgG) (Euroimmun, Lübeck, Germany)). The ichroma detected all 36 anti-CHIKV IgG and 57 anti-CHIKV IgM positivity (100% sensitivity). For 163 anti-CHIKV IgG/IgM negative sera, the ichroma showed one false positive for IgM (99.4% specificity). The ichroma showed no cross-reactivity and no interference. The ichroma demonstrated good diagnostic performance compared to the current ELISAs.
참고문헌 (18)
- Galo SS, "Development of in-house serological methods for diagnosis and surveillance of chikungunya", Rev Panam Salud Publica, 41, pp.e56~ (2017)
- Pialoux G, "Chikungunya, an epidemic arbovirosis", Lancet Infect Dis, 7, pp.319~327 (2007)
- Natrajan MS, "Beyond fever and pain : diagnostic methods for chikungunya virus", J Clin Microbiol, 57, pp.e00350~e00419 (2019)
- Jacobsen S, "External quality assessment studies for laboratory performance of molecular and serological diagnosis of chikungunya virus infection", J Clin Virol, 76, pp.55~65 (2016)
- "Chikungunya"[URL]
- Jeon BH, "Epidemiological characteristics of cases with Chikungunya fever in Korea from 2013 to 2018", Public Health Weekly Report, 12, pp.372~378 (2019)
- Mardekian SK, "Diagnostic options and challenges for dengue and chikungunya viruses", BioMed Res Int, 2015, pp.834371~ (2015)
- Burdino E, "Unmet needs for a rapid diagnosis of chikungunya virus infection", Emerg Infect Dis, 22, pp.1837~1839 (2016)
- Johnson BW, "Laboratory diagnosis of Chikungunya virus infections and commercial sources for diagnostic assays", J Infect Dis, 214(suppl 5), pp.S471~S474 (2016)
- Weaver SC, "Chikungunya virus and the global spread of a mosquito-borne disease", N Engl J Med, 372, pp.1231~1239 (2015)
- Prat CM, "Evaluation of commercially available serologic diagnostic tests for chikungunya virus", Emerg Infect Dis, 20, pp.2129~2132 (2014)
- Kosasih H, "Evaluation of two IgM rapid immunochromatographic tests during circulation of Asian lineage chikungunya virus", Southeast Asian J Trop Med Public Health, 43, pp.55~61 (2012)
- Rianthavorn P, "Evaluation of a rapid assay for detection of IgM antibodies to chikungunya", Southeast Asian J Trop Med Public Health, 41, pp.92~96 (2010)
- Yap G, "Evaluation of chikungunya diagnostic assays : differences in sensitivity of serology assays in two independent outbreaks", PLoS Negl Trop Dis, 4, pp.e753~ (2010)
- De Salazar PM, "Evaluation of three commercially-available chikungunya virus immunoglobulin G immunoassays", Rev Panam Salud Publica, 41, pp.e62~ (2017)
- Johnson BW, "Evaluation of commercially available chikungunya virus immunoglobulin M detection assays", Am J Trop Med Hyg, 95, pp.182~192 (2016)
- Fox JM, "Broadly neutralizing alphavirus antibodies bind an epitope on E2 and inhibit entry and egress", Cell, 163, pp.1095~1107 (2015)
- Abhishek KS, "Simultaneous detection of IgM antibodies against dengue and chikungunya : Coinfection or cross-reactivity", J Family Med Prim Care, 8, pp.2420~2423 (2019)