Molecular Surveillance of Pfelch13 and Pfmdr1 Mutations in Plasmodium falciparum Isolates from Southern Thailand
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Artemisinin-based combination therapy (ACT) resistance is widespread throughout the Greater Mekong Subre- gion. This raises concern over the antimalarial treatment in Thailand since it shares borders with Cambodia, Laos, and Myanmar where high ACT failure rates were reported. It is crucial to have information about the spread of ACT resistance for efficient planning and treatment. This study was to identify the molecular markers for antimalarial drug resistance: Pfkelch13 and Pfmdr1 mutations from 5 provinces of southern Thailand, from 2012 to 2017, of which 2 provinces on the Thai- Myanmar border (Chumphon and Ranong), one on Thai-Malaysia border (Yala) and 2 from non-border provinces (Phang Nga and Surat Thani). The results showed that C580Y mutation of Pfkelch13 was found mainly in the province on the Thai-Myanmar border. No mutations in the PfKelch13 gene were found in Surat Thani and Yala. The Pfmdr1 gene iso- lated from the Thai-Malaysia border was a different pattern from those found in other areas (100% N86Y) whereas wild type strain was present in Phang Nga. Our study indicated that the molecular markers of artemisinin resistance were spread in the provinces bordering along the Thai-Myanmar, and the pattern of Pfmdr1 mutations from the areas along the international border of Thailand differed from those of the non-border provinces. The information of the molecular markers from this study highlighted the recent spread of artemisinin resistant parasites from the endemic area, and the data will be useful for optimizing antimalarial treatment based on regional differences.
Abstract
Artemisinin-based combination therapy (ACT) resistance is widespread throughout the Greater Mekong Subre- gion. This raises concern over the antimalarial treatment in Thailand since it shares borders with Cambodia, Laos, and Myanmar where high ACT failure rates were reported. It is crucial to have information about the spread of ACT resistance for efficient planning and treatment. This study was to identify the molecular markers for antimalarial drug resistance: Pfkelch13 and Pfmdr1 mutations from 5 provinces of southern Thailand, from 2012 to 2017, of which 2 provinces on the Thai- Myanmar border (Chumphon and Ranong), one on Thai-Malaysia border (Yala) and 2 from non-border provinces (Phang Nga and Surat Thani). The results showed that C580Y mutation of Pfkelch13 was found mainly in the province on the Thai-Myanmar border. No mutations in the PfKelch13 gene were found in Surat Thani and Yala. The Pfmdr1 gene iso- lated from the Thai-Malaysia border was a different pattern from those found in other areas (100% N86Y) whereas wild type strain was present in Phang Nga. Our study indicated that the molecular markers of artemisinin resistance were spread in the provinces bordering along the Thai-Myanmar, and the pattern of Pfmdr1 mutations from the areas along the international border of Thailand differed from those of the non-border provinces. The information of the molecular markers from this study highlighted the recent spread of artemisinin resistant parasites from the endemic area, and the data will be useful for optimizing antimalarial treatment based on regional differences.
참고문헌 (46)
- "World Malaria Report 2015"[URL]
- Wongsrichanalai C, "Declining artesunate-mefloquine efficacy against falciparum malaria on the Cambodia-Thailand border", Emerg Infect Dis, 14, pp.716~719 (2008)
- "Strategy for malaria elimination in the Greater Mekong Subregion: 2015-2030"[URL]
- "President’s Malaria Initiative Greater Mekong Sub-region: Malaria Operational Plan FY 2017"[URL]
- Dondorp AM, "Artemisinin resistance in Plasmodium falciparum malaria", N Engl J Med, 361, pp.455~467 (2009)
- Dondorp A, "Artesunate versus quinine for treatment of severe falciparum malaria : a randomised trial", Lancet, 366, pp.717~725 (2005)
- Dondorp AM, "Artesunate versus quinine in the treatment of severe falciparum malaria in African children(AQUAMAT) : an open-label, randomised trial", Lancet, 376, pp.1647~1657 (2010)
- White N, "Antimalarial drug resistance and combination chemotherapy", Philos Trans R Soc Lond B Biol Sci, 354, pp.739~749 (1999)
- "Status Report on Artemisinin Resistance"[URL]
- Noedl H, "Evidence of artemisinin-resistant malaria in western Cambodia", N Engl J Med, 359, pp.2619~2620 (2008)
- "Asia Pacific Malaria Elimination Network 2014"[URL]
- Phyo AP, "Declining efficacy of artemisinin combination therapy against P. Falciparum malaria on the Thai-Myanmar border (2003-2013): the role of parasite genetic factors", Clin Infect Dis, 63, pp.784~791 (2016)
- Ariey F, "A molecular marker of artemisinin-resistant Plasmodium falciparum malaria", Nature, 505, pp.50~55 (2014)
- "Status Report on Artemisinin and ACT Resistance"[URL]
- Ashley EA, "Spread of artemisinin resistance in Plasmodium falciparum malaria", N Engl J Med, 371, pp.411~423 (2014)
- Holmgren G, "Amodiaquine resistant Plasmodium falciparum malaria in vivo is associated with selection of pfcrt 76T and pfmdr1 86Y", Infect Genet Evol, 6, pp.309~314 (2006)
- Sá JM, "Geographic patterns of Plasmodium falciparum drug resistance distinguished by differential responses to amodiaquine and chloroquine", Proc Natl Acad Sci USA, 106, pp.18883~18889 (2009)
- Reed MB, "Pgh1 modulates sensitivity and resistance to multiple antimalarials in Plasmodium falciparum", Nature, 403, pp.906~909 (2000)
- Sidhu AB, "pfmdr1 mutations contribute to quinine resistance and enhance mefloquine and artemisinin sensitivity in Plasmodium falciparum", Mol Microbiol, 57, pp.913~926 (2005)
- Sisowath C, "The role of pfmdr1 in Plasmodium falciparum tolerance to artemether-lumefantrine in Africa", Trop Med Int Health, 12, pp.736~742 (2007)
- Veiga MI, "Globally prevalent PfMDR1 mutations modulate Plasmodium falciparum susceptibility to artemisinin-based combination therapies", Nat Commun, 7, pp.11553~ (2016)
- Koenderink JB, "The ABCs of multidrug resistance in malaria", Trends Parasitol, 26, pp.440~446 (2010)
- Pickard AL, "Resistance to antimalarials in Southeast Asia and genetic polymorphisms in pfmdr1", Antimicrob Agents Chemother, 47, pp.2418~2423 (2003)
- Humphreys GS, "Amodiaquine and artemether-lumefantrine select distinct alleles of the Plasmodium falciparum mdr1 gene in Tanzanian children treated for uncomplicated malaria", Antimicrob Agents Chemother, 51, pp.991~997 (2007)
- Lekostaj JK, "A single S1034C mutation confers altered drug sensitivity to PfMDR1 ATPase activity that is characteristic of the 7G8 isoform", Mol Biochem Parasitol, 157, pp.107~111 (2008)
- Health indicators 2014. Malaysia: 2014, "Ministry of Health Malaysia", ~, pp.Report Number: ISSN 1511-4589 MOH (2014), S[DOI]
- Norahmad NA, "Prevalence of Plasmodium falciparum molecular markers of antimalarial drug resistance in a residual malaria focus area in Sabah, Malaysia", PLoS One, 11, pp.e0165515~ (2016)
- Snounou G, "High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction", Mol Biochem Parasitol, 61, pp.315~320 (1993)
- Basco LK, "Molecular epidemiology of malaria in Cameroon. X. Evaluation of pfmdr1 mutations as genetic markers for resistance to amino alcohols and artemisinin derivatives", Am J Trop Med Hyg, 66, pp.667~671 (2002)
- Fairhurst RM, "Understanding artemisinin-resistant malaria : what a difference a year makes", Curr Opin Infect Dis, 28, pp.417~425 (2015)
- Isozumi R, "Novel mutations in K13 propeller gene of artemisinin resistant Plasmodium falciparum", Emerg Infect Dis, 21, pp.490~492 (2015)
- Wurtz N, "Role of Pfmdr1 in in vitro Plasmodium falciparum susceptibility to chloroquine, quinine, monodesethylamodiaquine, mefloquine, lumefantrine, and dihydroartemisinin", Antimicrob Agents Chemother, 58, pp.7032~7040 (2014)
- Imwong M SK, "The spread of artemisinin-resistant Plasmodium falciparum in the Greater Mekong subregion : a molecular epidemiology observational study", Lancet Infect Dis, 17, pp.491~497 (2017)
- Miotto O, "Multiple populations of artemisinin-resistant Plasmodium falciparum in Cambodia", Nat Genet, 45, pp.648~655 (2013)
- Miotto O, "Genetic architecture of artemisinin-resistant Plasmodium falciparum", Nat Genet, 47, pp.226~234 (2015)
- Kobasa T, "Emergence and Spread of kelch13 Mutations Associated with Artemisinin Resistance in Plasmodium falciparum Parasites in 12 Thai Provinces from 2007 to 2016", Antimicrob Agents Chemother, 62, pp.e02141~e02117 (2018)
- Noedl H, "Evidence of artemisinin-resistant malaria in western Cambodia", N Engl J Med, 359, pp.2619~2620 (2008)
- Ye R, "Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border", Sci Rep, 6, pp.20100~ (2016)
- Putaporntip C, "Natural selection of K13 mutants of Plasmodium falciparum in response to artemisinin combination therapies in Thailand", Clin Microbiol Infect, 22, pp.285. e1~285. e8 (2016)[DOI]
- Talundzic E, "Selection and spread of artemisinin-resistant alleles in Thailand prior to the global artemisinin resistance containment campaign", PLoS Pathog, 11, pp.e1004789~ (2015)
- Ménard D, "A Worldwide Map of Plasmodium falciparum K13-Propeller Polymorphisms", N Engl J Med, 374, pp.2453~2464 (2016)
- Imwong M, "An outbreak of artemisinin resistant falciparum malaria in Eastern Thailand", Sci Rep, 5, pp.17412~ (2015)
- Mungthin M, "Distribution of pfmdr1 polymorphisms in Plasmodium falciparum isolated from Southern Thailand", Malar J, 13, pp.117~ (2014)
- Khim N, "Countrywide survey shows very high prevalence of Plasmodium falciparum multilocus resistance genotypes in Cambodia", Antimicrob Agents Chemother, 49, pp.3147~3152 (2005)
- Imwong M, "Exploring the contribution of candidate genes to artemisinin resistance in Plasmodium falciparum", Antimicrob Agents Chemother, 54, pp.2886~2892 (2010)
- Mungthin M, "Influence of the pfmdr1 gene on in vitro sensitivities of piperaquine in Thai isolates of Plasmodium falciparum", Am J Trop Med Hyg, 96, pp.624~629 (2017)