본문 바로가기
KERD
논문KCI 등재

Molecular Markers for Sulfadoxine/Pyrimethamine and Chloroquine Resistance in Plasmodium falciparum in Thailand

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Drug resistance is an important problem hindering malaria elimination in tropical areas. Point mutations in Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes confer resistance to antifolate drug, sulfadoxine-pyrimethamine (SP) while P. falciparum chloroquine-resistant transporter (Pfcrt) genes caused resistance to chloroquine (CQ). Decline in Pfdhfr/Pfdhps and Pfcrt mutations after withdrawal of SP and CQ has been reported. The aim of present study was to investigate the prevalence of Pfdhfr, Pfdhps, and Pfcrt mutation from 2 endemic areas of Thailand. All of 200 blood samples collected from western area (Thai-Myanmar) and southern area (Thai-Malaysian) contained multiple mutations in Pfdhfr and Pfdhps genes. The most prevalent haplotypes for Pfdhfr and Pfdhps were quadruple and double mutations, respectively. The quadruple and triple mutations of Pfdhfr and Pfdhps were common in western samples, whereas low frequency of triple and double mutations was found in southern samples, respectively. The Pfcrt 76T mutation was present in all samples examined. Malaria isolated from 2 different endemic regions of Thailand had high mutation rates in the Pfdhfr, Pfdhps, and Pfcrt genes. These findings highlighted the fixation of mutant alleles causing resistance of SP and CQ in this area. It is necessary to monitor the re-emergence of SP and CQ sensitive parasites in this area.

Abstract

Drug resistance is an important problem hindering malaria elimination in tropical areas. Point mutations in Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes confer resistance to antifolate drug, sulfadoxine-pyrimethamine (SP) while P. falciparum chloroquine-resistant transporter (Pfcrt) genes caused resistance to chloroquine (CQ). Decline in Pfdhfr/Pfdhps and Pfcrt mutations after withdrawal of SP and CQ has been reported. The aim of present study was to investigate the prevalence of Pfdhfr, Pfdhps, and Pfcrt mutation from 2 endemic areas of Thailand. All of 200 blood samples collected from western area (Thai-Myanmar) and southern area (Thai-Malaysian) contained multiple mutations in Pfdhfr and Pfdhps genes. The most prevalent haplotypes for Pfdhfr and Pfdhps were quadruple and double mutations, respectively. The quadruple and triple mutations of Pfdhfr and Pfdhps were common in western samples, whereas low frequency of triple and double mutations was found in southern samples, respectively. The Pfcrt 76T mutation was present in all samples examined. Malaria isolated from 2 different endemic regions of Thailand had high mutation rates in the Pfdhfr, Pfdhps, and Pfcrt genes. These findings highlighted the fixation of mutant alleles causing resistance of SP and CQ in this area. It is necessary to monitor the re-emergence of SP and CQ sensitive parasites in this area.

참고문헌 (41)

  1. Kesara Na-Bangchang, "Current Malaria Status and Distribution of Drug Resistance in East and Southeast Asia with Special Focus to Thailand", The Tohoku Journal of Experimental Medicine, 211(2), pp.99~113 (2007), Tohoku University Medical Press[DOI]
  2. Chansuda Wongsrichanalai, "Epidemiology of drug-resistant malaria", The Lancet Infectious Diseases, 2(4), pp.209~218 (2002), Elsevier BV[DOI]
  3. Harald Noedl, "Artemisinin-Resistant Malaria in Asia", New England Journal of Medicine, 361(5), pp.540~541 (2009), Massachusetts Medical Society[DOI]
  4. Tranakchit Harinasuta, "CHLOROQUINE-RESISTANT FALCIPARUM MALARIA IN THAILAND", The Lancet, 286(7414), pp.657~660 (1965), Elsevier BV[DOI]
  5. Martin D. Young, "Drug Resistance in Plasmodium Falciparum from Thailand", The American Journal of Tropical Medicine and Hygiene, 12(3), pp.305~314 (1963), American Society of Tropical Medicine and Hygiene[DOI]
  6. WILLIAM CHIN, "A COMPARATIVE EVALUATION OF SULFALENE-TRIMETHOPRIM AND SULPHORMETHOXINE-PYRIMETHAMINE AGAINST FALCIPARUM MALARIA IN THAILAND *", The American Journal of Tropical Medicine and Hygiene, 22(3), pp.308~312 (1973), American Society of Tropical Medicine and Hygiene[DOI]
  7. David E. Johnson, "Falciparum Malaria Acquired in the Area of the Thai-Khmer Border Resistant to Treatment with Fansidar® *", The American Journal of Tropical Medicine and Hygiene, 31(5), pp.907~912 (1982), American Society of Tropical Medicine and Hygiene[DOI]
  8. Wongsrichanalai C, "Drug resistant malaria on the Thai-Myanmar and Thai-Cambodian borders", Southeast Asian J Trop Med Public Health, 32, pp.41~49 (2001)
  9. "Malaria in the Greater Mekong Subregion: Regional and Country Profiles" (2008)[URL]
  10. John C. Reeder, "Point Mutations in the Dihydrofolate Reductase and Dihydropteroate Synthetase Genes and in Vitro Susceptibility to Pyrimethamine and Cycloguanil of Plasmodium falciparum Isolates from Papua New Guinea", The American Journal of Tropical Medicine and Hygiene, 55(2), pp.209~213 (1996), American Society of Tropical Medicine and Hygiene[DOI]
  11. Leonardo K. Basco, "Point mutations in the dihydrofolate reductase-thymidylate synthase gene and pyrimethamine and cycloguanil resistance in Plasmodium falciparum", Molecular and Biochemical Parasitology, 69(1), pp.135~138 (1995), Elsevier BV[DOI]
  12. Christopher V. Plowe, "Mutations in<i>Plasmodium falciparum<", i>Dihydrofolate Reductase and Dihydropteroate Synthase and Epidemiologic Patterns of Pyrimethamine‐Sulfadoxine Use and Resistance, 1997, pp.176(6), 1590~1596[DOI]
  13. Hamza A. Babiker, "High‐Level Chloroquine Resistance in Sudanese Isolates of<i>Plasmodium falciparum<", i>Is Associated with Mutations in the Chloroquine Resistance Transporter Gene<i>pfcrt<, i>, pp.The Journal of Infectious Diseases, 2001[DOI]
  14. James G. Kublin, "Reemergence of Chloroquine‐Sensitive<i>Plasmodium falciparum<", i>Malaria after Cessation of Chloroquine Use in Malawi, 2003, pp.187(12), 1870~1875[DOI]
  15. Asia Mohammed, "Trends in chloroquine resistance marker, Pfcrt-K76T mutation ten years after chloroquine withdrawal in Tanzania", Malaria Journal, 12(1), pp.415~ (2013), Springer Science and Business Media LLC[DOI]
  16. Leah Mwai, "Chloroquine resistance before and after its withdrawal in Kenya", Malaria Journal, 8(1), pp.106~ (2009), Springer Science and Business Media LLC[DOI]
  17. Wang X, "Decreased prevalence of the Plasmodium falciparum chloroquine resistance transporter 76T marker associated with cessation of chloroquine use against P. falciparum malaria in Hainan, People's Republic of China", Am J Trop Med Hyg, 72, pp.410~414 (2005)
  18. Andrea M. McCollum, "Common origin and fixation of Plasmodium falciparum dhfr and dhps mutations associated with sulfadoxine-pyrimethamine resistance in a low-transmission area in South America", Antimicrobial Agents and Chemotherapy, 51(6), pp.2085~2091 (2007), American Society for Microbiology[DOI]
  19. Elifaged Hailemeskel, "Prevalence of sulfadoxine–pyrimethamine resistance-associated mutations in dhfr and dhps genes of Plasmodium falciparum three years after SP withdrawal in Bahir Dar, Northwest Ethiopia", Acta Tropica, 128(3), pp.636~641 (2013), Elsevier BV[DOI]
  20. Richard J. Pearce, "A Community-Randomized Evaluation of the Effect of Intermittent Preventive Treatment in Infants on Antimalarial Drug Resistance in Southern Tanzania", The Journal of Infectious Diseases, 207(5), pp.848~859 (2012), Oxford University Press (OUP)[DOI]
  21. Raman J, "Differential effect of regional drug pressure on dihydrofolate reductase and dihydropteroate synthetase mutations in southern Mozambique", Am J Trop Med Hyg, 78, pp.256~261 (2008)[DOI]
  22. Nimol Khim, "Countrywide survey shows very high prevalence of Plasmodium falciparum multilocus resistance genotypes in Cambodia", Antimicrobial Agents and Chemotherapy, 49(8), pp.3147~3152 (2005), American Society for Microbiology[DOI]
  23. Andrea M. McCollum, "Common origin and fixation of Plasmodium falciparum dhfr and dhps mutations associated with sulfadoxine-pyrimethamine resistance in a low-transmission area in South America", Antimicrobial Agents and Chemotherapy, 51(6), pp.2085~2091 (2007), American Society for Microbiology[DOI]
  24. Duraisingh MT, "Plasmodium falciparum : detection of polymorphisms in the dihydrofolate reductase and dihydropteroate synthetase genes by PCR and restriction digestion", Exp Parasitol, 89, pp.1~8 (1998)[DOI]
  25. Abdoulaye Djimdé, "A Molecular Marker for Chloroquine-Resistant Falciparum Malaria", New England Journal of Medicine, 344(4), pp.257~263 (2001), Massachusetts Medical Society[DOI]
  26. Maguire JD, "The T76 mutation in the pfcrt gene of Plasmodium falciparum and clinical chloroquine resistance phenotypes in Papua, Indonesia", Ann Trop Med Parasitol, 95, pp.559~572 (2001)[DOI]
  27. Lemu Golassa, "Detection of a substantial number of sub-microscopic Plasmodium falciparum infections by polymerase chain reaction: a potential threat to malaria control and diagnosis in Ethiopia", Malaria Journal, 12(1), pp.352~ (2013), Springer Science and Business Media LLC[DOI]
  28. Tingting Jiang, "High prevalence of Pfdhfr–Pfdhps quadruple mutations associated with sulfadoxine–pyrimethamine resistance in Plasmodium falciparum isolates from Bioko Island, Equatorial Guinea", Malaria Journal, 18(1), pp.101~ (2019), Springer Science and Business Media LLC[DOI]
  29. Sukmawati Basuki, "Two novel mutations of pfdhps K540T and I588F, affecting sulphadoxine-pyrimethamine-resistant response in uncomplicated falciparum malaria at Banjar district, South Kalimantan Province, Indonesia", Malaria Journal, 13(1), pp.135~ (2014), Springer Science and Business Media LLC[DOI]
  30. Lau TY, "Mutational analysis of Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase genes in the interior division of Sabah, Malaysia", Malar J, 12, pp.445~ (2013)[DOI]
  31. Yan Zhao, "Genetic Variations Associated with Drug Resistance Markers in Asymptomatic Plasmodium falciparum Infections in Myanmar", Genes, 10(9), pp.692~ (2019), MDPI AG[DOI]
  32. Md Tauqeer Alam, "Tracking origins and spread of sulfadoxine-resistant Plasmodium falciparum dhps alleles in Thailand", Antimicrobial Agents and Chemotherapy, 55(1), pp.155~164 (2011), American Society for Microbiology[DOI]
  33. Rungniran Sugaram, "Molecular characterization of Plasmodium falciparum antifolate resistance markers in Thailand between 2008 and 2016", Malaria Journal, 19(1), pp.107~ (2020), Springer Science and Business Media LLC[DOI]
  34. Mallika Imwong, "An outbreak of artemisinin resistant falciparum malaria in Eastern Thailand", Scientific Reports, 5(1), pp.17412~ (2015), Springer Science and Business Media LLC[DOI]
  35. Sivicha Krudsood, "Artesunate–dapsone–proguanil treatment of falciparum malaria: genotypic determinants of therapeutic response", Transactions of the Royal Society of Tropical Medicine and Hygiene, 99(2), pp.142~149 (2005), Oxford University Press (OUP)[DOI]
  36. Chavachol Setthaudom, "Role of Plasmodium falciparum Chloroquine Resistance Transporter and Multidrug Resistance 1 Genes on In Vitro Chloroquine Resistance in Isolates of Plasmodium falciparum from Thailand", The American Journal of Tropical Medicine and Hygiene, 85(4), pp.606~611 (2011), American Society of Tropical Medicine and Hygiene[DOI]
  37. Gabrielle Holmgren, "Amodiaquine resistant Plasmodium falciparum malaria in vivo is associated with selection of pfcrt 76T and pfmdr1 86Y", Infection, Genetics and Evolution, 6(4), pp.309~314 (2006), Elsevier BV[DOI]
  38. Nanhua Chen, "pfcrt allelic types with two novel amino acid mutations in chloroquine-resistant Plasmodium falciparum isolates from the Philippines", Antimicrobial Agents and Chemotherapy, 47(11), pp.3500~3505 (2003), American Society for Microbiology[DOI]
  39. Dinora Lopes, "Molecular characterisation of drug-resistant Plasmodium falciparum from Thailand", Malaria Journal, 1(1), pp.12~ (2002), Springer Science and Business Media LLC[DOI]
  40. K. Congpuong, "Molecular epidemiology of drug resistance markers of Plasmodium falciparum malaria in Thailand", Tropical Medicine and International Health, 10(8), pp.717~722 (2005), Wiley[DOI]
  41. Kanchana Rungsihirunrat, "Association between chloroquine resistance phenotypes and point mutations in pfcrt and pfmdr1 in Plasmodium falciparum isolates from Thailand", Acta Tropica, 109(1), pp.37~40 (2009), Elsevier BV[DOI]

관련 논문AI추천

Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of ThailandJiraporn Kuesap, Kanchana Rungsihirunrat1, Kesara Na Bangchang, Poonuch Muhamad2, Wanna Chaijaroenkul대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제1호Evolution of genetic polymorphisms of Plasmodium falciparum merozoite surface protein (PfMSP) in ThailandJiraporn Kuesap대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2014 · 제52권 제1호Molecular Surveillance of Pfelch13 and Pfmdr1 Mutations in Plasmodium falciparum Isolates from Southern ThailandAung Win Tun, Hansuk Buncherd, Nongyao Sawangjaroen, Supinya Thanapongpichat, Thunchanok Khammanee대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2019 · 제57권 제4호Genetic Polymorphisms in Plasmodium vivax Dihydrofolate Reductase and Dihydropteroate Synthase in Isolates from the Philippines, Bangladesh, and NepalEffie Espino, Harald Noedl, Jiraporn Kuesap, Kanchana Rungsihirunrat, Kesara Na-Bangchang, Pimwan Thongdee, Shyam Prakash Dumre대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2015 · 제53권 제2호Plasmodium falciparum genotype diversity in artemisinin derivatives treatment failure patients along the Thai-Myanmar borderCongpuong, Kanungnit대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2014 · 제52권 제6호Plasmodium vivax dhfr Mutations among Isolates from Malarious Areas of IranAbbas Shahbazi, Jalal Zaman, Mohammad Asgharzadeh대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2011 · 제49권 제2호

이 간행물의 다른 논문 (7건)