중금속에 의한 대장균 성장 저해의 정량적 평가
Quantification of Inhibitory Impact of Heavy Metals on the Growth of Escherichia coli.
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초록
Escherichia coli의 생장을 저해하는 중금속(Cu, Cd, Cr, Hg, Zn) 영향을 정량적으로 조사하였고, 중금속 독성에 대한 적응과정에서의 독성변화를 노출시간대별로 평가하였다. 중금속에 의한 E. coli의 생장저해 작용은 중금속의 종류에 따라 다른 동향을 보였고, 생장을 완전 저해하는 중금속의 농도는 각각 Cu 3.5 mM, Zn 2.5 mM, Cd 1.5 mM, Cr 1.2 mM, Hg 0.12 mM 이었다. EC50을 기준으로 한 E. coli의 중금속에 대한 내성은 Cu > Zn > Cr > Cd > Hg 순이다. 노출 시간에 따른 EC50의 변화를 plotting하여 그 기울기에 따라 피시험체의 독성물질에 대한 적응성 평가가 가능함을 보였다. 기울기 값이 작은 경우에는 피검사체가 독성물질에 대한 적응성이 약하고, 기울기 값이 크면 클수록 피시험체가 독성물질에 대한 적응성이 우수하다는 것을 의미한다. E. coli의 독성물질인 중금속에 대한 적응성은 Zn > Cd > Cu ≫ Cr > Hg 순 이었다.
Abstract
The quantification of the toxicological effects of the heavy metals such as Cu, Cd, Cr, Hg, and Zn on the growth of Escherichia coli was performed, and the variations of toxicities with exposure time were evaluated in adaptation procedure. The characteristics of growth inhibition on Escherichia coli by heavy metals were different with metal species, and critical concentration of each metal, which inhibited cell growth completely, were Cu of 3.5 mM, Zn of 2.5 mM, Cd of 1.5 mM, Cr of 1.2 mM, and Hg of 0.12 mM, respectively. The tolerance of E. coli against heavy metals, based on EC50 values, increased in order of Cu > Zn > Cr > Cd > Hg. The slopes obtained from the relationship between EC50 values and expose time corresponds to adaptability of test organisms to the toxicants. The adaptability of test organisms to the toxicants was much higher at higher slope values. Adaptability of E. coli on heavy metals increased in order to Zn > Cd > Cu ≫ Cr > Hg.
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