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RBL-2H3에서 IgE-dependent Histamine-releasing Factor에 의한 활성산소종 생성에 관한 연구

Generation of ROS by IgE-Dependent Histamine-Releasing Factor in RBL-2H3 Cells.

이경림|주이신
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2005.09|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제33권 제3호|pp. 231-235

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참고문헌

주제분류생물학

초록

RBL-2H3 cell에서 HRF에 의하여 histamine이 분비되는 과정에서 ROS가 생성되는지 실험해 본 결과, ROS가 HRF를 처리한지 5분대에 최대치를 보이며 생성되었다가 소멸되는 것을 관찰할 수 있었다. 따라서 HRF가 세포내 second messenger로써의 ROS를 생성하였다고 확인할 수 있었다. 또한 ROS는 단백질 정제 과정에서의 endotoxin 오염에 의해 영향을 받지만, 본 실험에서 규명한 HRF에 의한 ROS생성은 endotoxin에 의한 것이 아닌, 순수하게 HRF에 의한 signaling의 결과라는 것도 확인할 수 있었다.

Abstract

Histamine-releasing factors (HRFs) are soluble mediators that can release histamine and other mediators from basophils and mast cells and their activity can vary, depending on the type of IgE. The activity of HRFs is affected by the presence of IgE, although HRF is thought to bind to a specific receptor other than IgE. Until now, HRF signaling pathway including its receptor remains unclear in spite of numerous studies. Since there had been many reports about re- active oxygen species (ROS) as a signaling molecule rather than as a by-product of metabolism, we investigated the possibility of ROS as an intracellular messenger involved in HRF-mediated histamine degranulation. In RBL-2H3 cells, ROS was generated by HRF using H2O2-sensitive fluorescence of fluorescent 2’, 7’-dichlorofluorescein (H2DCFDA). These effects were blocked by anti-oxidant N-acetylcysteine (NAC). These results suggest that ROS generation could play a role as an intracellular messenger in histamine release by HRF

참고문헌 (23)

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