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Helicobacter pylori 억제능김치유산균의분리와특성규명

Isolation and Characterization of Kimchi Lactic Acid Bacteria Showing Anti-Helicobacter pylori Activity.

장해춘|이율
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2008.06|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제36권 제2호|pp. 106-114

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주제분류생물학

초록

One bacterium, which showed strong antagonistic activity against H. pylori KCCM 41756, was isolated from kimchi. The strain NO1 was designated as Lactobacillus plantarum NO1 based on Gram staining, biochemical properties, and 16S rRNA gene sequencing. The culture medium (2~4 μg/ml) of Lb. plantarum NO1 reduced (40~60%) the urease activity of H. pylori KCCM 41756. Lb. plantarum NO1 inhibited the binding of H. pylori to human gastric cancer cell line, AGS cells, by more than 33%. Lb. plantarum NO1 exhibited high viability (maintained initial viable cell count of 109 CFU/ml) in 0.05 M sodium phosphate buffer (pH 3.0) for 2 h, in artificial gastric juice for 2 h and in 0.3%, 0.5% oxgall for 24 h. Hemolysis phenomena did not observed when Lb. plantarum NO1 was incubated in the blood agar media. We concluded that Lb.plantarum NO1 can be a good candidate as a probiotic, harboring anti-H. pylori activity.

Abstract

One bacterium, which showed strong antagonistic activity against H. pylori KCCM 41756, was isolated from kimchi. The strain NO1 was designated as Lactobacillus plantarum NO1 based on Gram staining, biochemical properties, and 16S rRNA gene sequencing. The culture medium (2~4 μg/ml) of Lb. plantarum NO1 reduced (40~60%) the urease activity of H. pylori KCCM 41756. Lb. plantarum NO1 inhibited the binding of H. pylori to human gastric cancer cell line, AGS cells, by more than 33%. Lb. plantarum NO1 exhibited high viability (maintained initial viable cell count of 109 CFU/ml) in 0.05 M sodium phosphate buffer (pH 3.0) for 2 h, in artificial gastric juice for 2 h and in 0.3%, 0.5% oxgall for 24 h. Hemolysis phenomena did not observed when Lb. plantarum NO1 was incubated in the blood agar media. We concluded that Lb.plantarum NO1 can be a good candidate as a probiotic, harboring anti-H. pylori activity.

참고문헌 (32)

  1. 정후길, "Helicobacter pylori의 생육을 특이적으로 억제하는 유산균 선발", 한국미생물학회, 37, pp.151~157 (2001), 한국미생물학회
  2. Armuzzi, A., "The effect of oral administration of Lactobacillus GG on antibioticassociated gastrointestinal side-effects during Helicobacter pylori eradication therapy", Aliment Pharmacol Ther, 15, pp.163~169 (2001)
  3. Bae, E. A., "Anti-Helicobacter pylori activity of Bifidobacterium spp", J. Microbiol. Biotechnol, 10, pp.532~534 (2000)
  4. Baek, Y. J, "Lactic acid bacteria and human health", Kor. J. Food Nutr, 6, pp.53~65 (1993)
  5. Chou, L. S, "Isolation and characterization of acid- and bile-tolerant isolates from strains of Lactobacillus acidophilus", J. Dairy Sci, 82, pp.23~31 (1999)
  6. 조영수, "동치미 유래 생균제로서 가능성이 있는 Lactobacillus sp. FF-3의 분리와 특성", 한국식품저장유통학회지, 10(3), pp.406~410 (2003), 한국식품저장유통학회
  7. Coconnier, M. H., "Antagonistic activity against Helicobacter infection in vitro and in vivo by the human Lactobacillus acidophilus strain LB", Appl. Environ. Microbiol, 64, pp.4573~4580 (1998)
  8. Drahoslava, L., "Helicobacter pylori and probiotics", J. Nutr, 137, pp.812~818 (2007)
  9. Kang, J. H., "In vitro inhibition of Helicobacter pylori by Enterococcus faecium GM-1", Can. J. Microbiol, 51, pp.629~636 (2005)
  10. Jeung-Ha Kil, "Effects of Kimchi on Stomach and Colon Health of Helicobacter pylori-Infected Volunteers", Preventive Nutrition and Food Science, 9(2), pp.161~166 (2004), 한국식품영양과학회
  11. 김은아, "유산균에 의한 Escherichia coli 와 Salmonella typhimurium의 생육억제에 관한 연구", 한국동물자원과학회지, 44(4), pp.491~498 (2002), 한국동물자원과학회
  12. 김효주, "김치로부터 Exopolysaccharide 생성 유산균의 분리 및 특성 규명", 한국미생물·생명공학회지, 34(3), pp.196~203 (2006), 한국미생물·생명공학회
  13. Kim, N. Y., "The effect of antibiotic resistance on the eradication of Helicobacter pylori", Kor. J. Gastroenterol, 47, pp.82~86 (2006)
  14. Kim, T. S., "Antagonism of Helicobacter pylori by bacteriocins of lactic acid bacteria", J. Food Protection, 66, pp.3~12 (2003)
  15. Kim, Y. M., "Physiological characteristics of Listeria monocytogenes YM- 7", J. Kor. Fish Soc, 28, pp.443~450 (1995)
  16. Kobayashi, Y., "Studies on biological characteristics of Lactobacillus: II. tolerance of the multiple antibiotic resistance strain, L. casei PSR3002, to artificial digestive fluids", Jpn. J. Microbiol, 29, pp.691~698 (1974)
  17. Koo, J. K., "Studies on adherance inhibition and detachment of Helicobacter pylori using egg yolk Igy and additives", Kor. J. Biotechnol. Bioeng, 16, pp.41~47 (2001)
  18. LEE, HAK MEE, "Isolation of Lactobacillus plantarum from Kimchi and Its Inhibitory Activity on the Adherence and Growth of Helicobacter pylori", Journal of Microbiology and Biotechnology, 16(10), pp.1513~1517 (2006), 한국미생물·생명공학회
  19. Lee, H. S., "Viablility in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from kimchi", Kor. J. Appl. Microbiol. Biotechnol, 25, pp.617~622 (1997)
  20. Lee, H. S., "Antitumor effect of cell wall component purified from Lactobacillus plantarum KK", J. Gen. Eng, 5, pp.22~28 (1993)
  21. Lee, S. Y., "Usefulness of probiotics against Helicobacter pylori", Safe. Food, 1, pp.44~52 (2006)
  22. Lee, Y., "Isolation and characterization of lactic acid bacteria showing anti-cancer activity and anti-Helicobacter pylori", M. S., pp.2007
  23. Midolo, P. D., "In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria", J. Appl. Bacteriol, 79, pp.475~479 (1995)
  24. 윤정원, "돼지분변으로부터 분리한 유산균주들의 헬리코박터 저해력과 항균활성 및 배양특성", KSBB Journal, 20(2), pp.76~83 (2005), 한국생물공학회
  25. 백현동, "식품용 프로바이오틱 비스루트균의 주요 생균제 특성", 한국식품과학회지, 34(1), pp.73~78 (2002), 한국식품과학회
  26. 박종길, "한국인 유아 분변에서 분리한 Lactobacillus acidophilus KY1909의 프로바이오틱 특성", 한국식품과학회지, 35(6), pp.1244~1247 (2003), 한국식품과학회
  27. Park, M. J., "The suppressive effect of a fermented milk containing Lactobacilli on Helicobacter pylori in human gastric mucosa", Kor. J. Gastroenterol, 38, pp.233~240 (2001)
  28. Rokka, S., "vitro growth inhibition of Helicobacter pylori by Lactobacilli belonging to the Lactobacillus plantarum group", Appl. Microbiol. Lett, 43, pp.508~513 (2006)
  29. Servin, A. L, "Antagonistic activity against Helicobacter pylori infection by the lactic acid bacteria", J. Kor. Public Health. Assoc, pp.5~12 (2001)
  30. Shin, M. S., "Characteristics of Bifidobacterium spp. isolated from korean feces for probiotics", Kor. J. Dairy Sci, 20, pp.273~282 (1998)
  31. Sim, J. H., "Comparative tests on the acid tolerance of some lactic acid bacteria species isolated from lactic fermented products", Kor. J. Food Sci. Technol, 27, pp.101~104 (1995)
  32. Won, B. R., "Inhibitory effect of Lactobacillus heiviticus CU631 on urease and vacuolating toxin activity of Helicobacter pyl", J. Anim. Sci. Technol, 43, pp.931~940 (2001)

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