미생물제제시용 고추경작지로부터 식물생장홀몬과 항진균물질을 동시에 생산하는 식물생장촉진근권세균의 선발 및 동정
Selection and Identification of Phytohormones and Antifungal Substances Simultaneously Producing Plant Growth Promoting Rhizobacteria from Microbial Agent Treated Red-pepper Fields
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초록
식물생장 촉진 홀몬 auxin을 생산하는 균주를 선발하기위해 경북 경산시 소재 고추경작지 근권토양으로 부터 739종의 일반호기성 균주와 urease 생산 균주 80종 및 광합성균주 303종과 같이 총 1000여종 이상의 균주를 분리하였다. 이 균주들을 대상으로 Salkowski test를 실시한 결과, auxin 을 생산하는 158종의 일반호기성 균주와 70종의 urease 생산 균주 및 228종의 광합성 균주를 선발할 수 있었으며, Holbrook test를 통해 또 다른 식물생장 촉진 호르몬인gibberellin도 대부분의 균주에서 생산되는 것을 확인할 수있었다. 선발된 균주 중 항진균 물질인 β-Glucanase와siderophore를 생산하고 다양한 병원성 진균에 대해 길항 범위를 가지는 6가지 균주 BCB14, BCB17, C10, HA46, HA143, HJ5를 toothpicking 및 대치배양을 통해 최종 선발할 수 있었으며, 분류학적으로 동정한 결과 6종 모두 B. subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, B. safensis HJ5로 확인되었다.
Abstract
In this study, a total of more than 1,000 bacteria, including 739 species of aerobic bacteria, 80 species of urease producing bacteria and 303 species of photosynthetic bacteria, were isolated from red-pepper field soils located in the Gyeongsan Province of the Republic of Korea. Amongst these, 158 species of aerobic bacteria, 70 species of urease producing bacteria and 228 species of photosynthetic bacteria were found to be auxin producing soil bacteria through quantification analysis with the Salkowski test. The latter groupings were then tested for antifungal activities to β-Glucanase and siderophore using CMC congo red agar and CAS blue agar media. In addition, the selected strains were examined for antifungal activity against various phytopathogenic fungi on PDN agar media. Six strains; BCB14, BCB17, C10, HA46, HA143, and HJ5, were noted for their ability to both produce auxin and act as antifungal substances. 16S rDNA sequence comparison analyses of these six strains identified them as Bacillus subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, and B. safensis HJ5.
참고문헌 (29)
- Amara, M. A. T, "Effect of inoculation with plant growth promoting rhizobacteria(PGPR) on yield and uptake of nutrients by wheat grown on sandy soil. Egyptian", J. Soil Science, 37, pp.467~484 (1997)
- Ates, S, "Effect of silicone oil on gibberellic acid production by Gibberella fujikuroi and Aspergillus niger", Appl. Biochem. Microbiol, 42, pp.500~501 (2006)
- Barthe, P, "Structural analysis of cassiicolin, a host-selective protein toxin from Corynespora cassiicola", J. Mol. Biol, 367, pp.89~101 (2007)
- Bomke, C, "Diversity, regulation, and evolution of the gibberellin biosynthetic pathway in fungi compared to plants and bacteria", Phytochemistry, 70, pp.1876~1893 (2009)
- Chen, W, "The role of microbial activity in suppression of daming-off caused by Pythium ultimum", Phytopathology, 78, pp.314~322 (1988)
- Chen, Y, "The use of yield increasing bacteria (YIB) as plant growthpromoting rhizobacteria in chinese agriculture, In Management of Soil Borne Diseases,", pp.165~184 (1994), Kalyani Publishers
- Choi, J. G, "Urease gene transfer of antagonistic bacillus subtilis YB-70 and increased antagonistic effect", Kor. J. Microbiol. Biotechnol, 25, pp.30~36 (1997)
- 김세일, "EzTaxon: A web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences", International Journal of Systematic and Evolutionary Microbiology, 57(10), pp.2259~2261, Society for General Microbiology
- Esitken, A, "Potential use of plant growth promoting rhizobacteria (PGPR) in organic apricot production", Proc. Int. Sci. Conf, pp.90~97 (2005), Environmentally Friendly Fruit Growing Tartu Estonia
- Imhoff, J. F, "Purple nonsulfur bacteria. pp. 1658-1662, In Bergey’s manual of systematic bacteriology. Vol. 3", pp.1658~1662 (1989), Williams and Wilkins
- Ishimaru, C. A, "High-affinity iron uptake systems present in Erwinia carotovora subsp. carotovora include the hydroxamate siderophore aerobactin", J. bacteriol, 174, pp.2993~3003 (1992)
- 정희경, "Auxin과 Siderophore 생산성 다기능 생물방제균Bacillus subtilis AH18", 한국미생물·생명공학회지, 34(2), pp.94~100 (2006), 한국미생물·생명공학회
- 정희경, "Auxin, Siderophore, 및 Cellulase 생산성 다기능 식물생장촉진미생물Bacillus licheniformis K11 의 선발 및 식물생장촉진 효과", Journal of the Korean Society for Applied Biological Chemistry, 50(1), pp.23~28 (2007), 한국응용생명화학회
- Khalid, A, "Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat", J. Appl. Microbiol, 96, pp.473~480 (2004)
- Kim, B. S, "Isolation and identification of Myxobacteria KR025 and searching of their bioactive compounds", Kor. J. Plant Pathol, 14, pp.345~349 (1998)
- Isolation of cellulase-producing plant growth promoting rhizobacteria in pepper farming soil, "Kim, J. W", Yeungnam University., ~ (2011)
- Kistler, H. C, "Genetic transformation of the fungal plant wilt pathogen, Fusarium oxysporum", Curr. Genet, 13, pp.145~149 (1988)
- Kousik, C. S, "Comparison of double-stranded RNA components and virulence among isolates of Rhizoctonia solani AG-1 IA and AG-1 IB", Phytopathology, 84, pp.44~49 (1993)
- Lee, J. M, "Isolation of siderophore-producing Pseudomonas fluorescens GL7 and its biocontrol activity against root-rot disease", Kor. J. Appl. Microbiol. Biotechnol, 27, pp.427~432 (1999)
- Isolation and identification of siderophore-producing bacteria that has antifungal activity, "Lee, K. E", Yeungnam University, ~ (2011)
- Gutierrez-Manero, F. J, "The plant growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellins", Physiol. Plant, 111(206)통권 : 211 (2001)
- Loon, L. C. van, "Plant responses to plant growthpromoting rhizobacteria", Eur. J. Plant Pathol, 119, pp.243~254 (2007)
- Lumsden, R. D, "In The Fungal Community: Ecology of mycoparasitism", pp.295~328 (1981), Marcel Dekker Inc
- Moon, S. W, "N2 fixation and H2 production by a phototrophic bacterium, Chromatium sp", Kor. J. Aquaculture, 10, pp.69~76 (1997)
- Isolation and identification of auxin and gibberellin producing photosynthetic bacteria in pepper farming, "Shon, H. J", Yeungnam University, ~ (2011)
- Tamura, K, "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0", Mol. Biol. Evol, 24, pp.1596~1599 (2007)
- Teather, R, "Use of congo red-polysaccharide interations in enumeration and characterization of cellulolytic bacteria from the bovine rumen", Appl. Environ. Microbiol, 43, pp.777~780 (1982)
- Thomas, O, "IAA synthesis in the biocontrol strain CHO of Pseudomonas fluorescens: role of tryptophan side chain oxidase", J. Gen. Microbiol, 137, pp.2273~2279 (1991)
- Xu, J., "Antifungal activity of oligochitosan against Phytophthora capsici and other plant pathogenic fungi in vitro", Pesticide Biochem. Physiol, 87, pp.220~228 (2007)