Inhibitory Effect of Transition Metal Gallium [Ga(NO3)3] on Biofilm Formation by Fish Pathogens
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
The prevalence of pathogenic bacteria such as Streptococcus parauberis (Sp), Streptococcus iniae (Si), and Edwardsiella tarda (Et) in flounder fish farms in Jeju Island and their management by gallium treatment was studied. Sp, Si, and Et were found to exhibit a low rate of cell growth and high biofilm formation. Hence, in the present study, cell growth and biofilm formation were measured spectrophotometrically 72 h after the addition of different concentrations of gallium (2, 4, or 8 mg/ml). In addition, cell death was measured by resazurin and propidium iodide staining assays. The results showed that bacterial cell death increased and biofilm formation decreased with an increasing concentration of gallium. Hence, the present study signifies that the use of gallium against bacterial pathogens could be useful for disease management in flounder farms.
Abstract
The prevalence of pathogenic bacteria such as Streptococcus parauberis (Sp), Streptococcus iniae (Si), and Edwardsiella tarda (Et) in flounder fish farms in Jeju Island and their management by gallium treatment was studied. Sp, Si, and Et were found to exhibit a low rate of cell growth and high biofilm formation. Hence, in the present study, cell growth and biofilm formation were measured spectrophotometrically 72 h after the addition of different concentrations of gallium (2, 4, or 8 mg/ml). In addition, cell death was measured by resazurin and propidium iodide staining assays. The results showed that bacterial cell death increased and biofilm formation decreased with an increasing concentration of gallium. Hence, the present study signifies that the use of gallium against bacterial pathogens could be useful for disease management in flounder farms.
참고문헌 (19)
- thymidine incorporation assay, "J. Immunol. Method", 1994, 211~224
- Banin E, "The potential of desferrioxamine-gallium as an anti-Pseudomonas therapeutic agent", Proc. Natl. Acad. Sci. USA, 105, pp.16761~16766 (2008)
- Chiang WC, "Extracellular DNA Shields against Aminoglycosides in Pseudomonas aeruginosa Biofilms", Antimicrob. Agents Chemother, 57, pp.2352~2361 (2013)
- De Fries R, "Quantification of mitogen induced human lymphocyte proliferation: Comparison of alamarbluetm assay to 3h‐thymidine incorporation assay", J. Clin. Lab. Anal, 9, pp.89~95 (1995)
- Deleon K, "Gallium maltolate treatment eradicates Pseudomonas aeruginosa infection in thermally injured mice", Antimicrob. Agents Chemother, 53, pp.1331~1337 (2009)
- 임진택, "Alamar Blue 색소의 환원량 평가에 의한 급성기 반응중 육계병아리의 비장세포와 PBMC 증식도 측정", 한국동물자원과학회지, 49(2), pp.213~224 (2007), 한국동물자원과학회
- 정승희, "Oxolinic acid의 경구투여, 주사 및 약욕에 따른 넙치, Paralichthys olivaceus 체내 약물동태학적 특성", 한국어병학회지, 22(2), pp.125~135 (2009), 한국어병학회
- Kaneko Y, "The transition metal gallium disrupts Pseudomonas aeruginosa iron metabolism and has antimicrobial and antibiofilm activity", J. Clin. Invest, 117, pp.877~888 (2007)
- Kim, Jong-Hyun, "Molecular Characterization of Regulatory Genes Associated with BiofilmVariation in a Staphylococcus aureus Strain", Journal of Microbiology and Biotechnology, 18(1), pp.28~34 (2008), 한국미생물·생명공학회
- 이창훈, "제주지역 양식 넙치 (Paralichthys olivaceus)로부터 분리되는 Streptococcus iniae와 Streptococcus parauberis의 생물학적 특성", 한국어병학회지, 20(1), pp.33~40 (2007), 한국어병학회
- 이정현, "미생물암에 대하여: 한국적 관점", 암석학회지, 24(4), pp.291~305 (2015), 한국암석학회[DOI]
- Martin A, "Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: Rapid, simple, and inexpensive methods", Antimicrob. Agents Chemother, 47, pp.3616~3619 (2003)
- Ministry for Food Agriculture Forestry and Fisheries, "Statistical year book of maritime affairs and fisheries", ~ (2014)
- Prevention of bacterial fish disease and medical treatment for produce health fish, "National Fisheries Research & Development Institute", ~ (2015)
- Nguyen HT, "Selective agars for the isolation of Streptococcus iniae from Japanese flounder, Paralichthys olivaceus, and its cultural environment", J. Appl. Microbiol, 86, pp.769~776 (1999)
- Kwi-Ok Oh, "Prevention of Biofilm Infections", Journal of Bacteriology and Virology, 39(4), pp.237~246 (2009), 대한미생물학회
- Park JS, "Purification siderophore from Vibrio mimicus ATCC 33653 and its effect to bacterial pathogenicity", J. Korean Soc. Microbiol, 34, pp.461~470 (1999)
- Aquaculture Status Survey, "Statistics Korea", ~ (2015)
- 우승호, "어류 병원성 세균 공격 후 마늘, Allium sativum 착즙액의 침지가 넙치, Paralichthys olivaceus 의 면역반응에 미치는 영향", 한국어병학회지, 23(2), pp.199~209 (2010), 한국어병학회