In vitro antimicrobial properties of Bacillus subtilis KCTC 1326 for fish bacterial disease management
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This study evaluated in vitro antimicrobial properties of Bacillus subtilis KCTC 1326 as an environ-mentally friendly alternative to antibiotics. B. subtilis KCTC 1326 was characterized on biochemical properties and antibiotics susceptibility. It exhibited antimicrobial effects against all 12 species of fish bacteria used in this experiment. Among them, the largest antibacterial zone was observed for Streptococcus parauberis (34 mm), while the smallest antibacterial zone was observed for Citrobacter freundii (8 mm). Additionally, in the co-culture inhibitory assay of B. subtilis and Edwardsiella piscici-da, the growth of E. piscicida was suppressed with increasing concentrations of B. subtilis KCTC 1326, with complete inhibition observed at 107 and 108 CFU/mL of B. subtilis KCTC 1326 after 24 hours of incubation. Moreover, at 48 hours of incubation, the growth of E. piscicida was inhibited across all concentration ranges of B. subtilis KCTC 1326. Therefore, this study indicated the utilizing of B. subtilis KCTC 1326 as an antimicrobial for controlling fish bacterial diseases.
Abstract
This study evaluated in vitro antimicrobial properties of Bacillus subtilis KCTC 1326 as an environ-mentally friendly alternative to antibiotics. B. subtilis KCTC 1326 was characterized on biochemical properties and antibiotics susceptibility. It exhibited antimicrobial effects against all 12 species of fish bacteria used in this experiment. Among them, the largest antibacterial zone was observed for Streptococcus parauberis (34 mm), while the smallest antibacterial zone was observed for Citrobacter freundii (8 mm). Additionally, in the co-culture inhibitory assay of B. subtilis and Edwardsiella piscici-da, the growth of E. piscicida was suppressed with increasing concentrations of B. subtilis KCTC 1326, with complete inhibition observed at 107 and 108 CFU/mL of B. subtilis KCTC 1326 after 24 hours of incubation. Moreover, at 48 hours of incubation, the growth of E. piscicida was inhibited across all concentration ranges of B. subtilis KCTC 1326. Therefore, this study indicated the utilizing of B. subtilis KCTC 1326 as an antimicrobial for controlling fish bacterial diseases.
참고문헌 (28)
- Abarike, E. D., "Effects of a commercial probiotic BS containing Bacillus subtilis and Bacillus licheniformis on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus", Fish & shellfish immunology, 82, pp.229~238 (2018)[DOI]
- Abriouel, H., "Diversity and applications of Bacillus bacteriocins", FEMS microbiology reviews, 35(1), pp.201~232 (2011)[DOI]
- Adesiyan, I. M., "Incidence and antimicrobial susceptibility fingerprints of Plesiomonas shigelliodes isolates in water samples collected from some freshwater resources in Southwest Nigeria", Science of the total environment, 665, pp.632~640 (2019)[DOI]
- Anagnostopoulos, C., "Requirements for transformation in Bacillus subtilis", Journal of Bacteriology, 81, pp.741~746 (1961)[DOI]
- Blibech, M., "Selection of Bacillus subtilis US191 as a mannanase-producing probiotic candidate", Biotechnology and Applied Biochemistry, 66(5), pp.858~869 (2019)[DOI]
- Caulier, S., "Overview of the antimicrobial compounds produced by members of the Bacillus subtilis group", Frontiers in microbiology, 10, pp.435128~ (2019)[DOI]
- Costa, T. S., "Use of Bacillus subtilis multiplicate in the water used for biofloc formation : Growth, hemato-biochemistry, intestinal bacteria colonies, and bacterial resistance evaluations of Nile tilapia", Aquaculture, 590, pp.741039~ (2024)[DOI]
- De Souza, D. M., "The use of probiotics during the nursery rearing of the pink shrimp Farfantepenaeus brasiliensis(Latreille, 1817)in a zero exchange system", Aquaculture Research, 43(12), pp.1828~1837 (2012)[DOI]
- El-Saadony, M. T., "The functionality of probiotics in aquaculture : An overview", Fish & shellfish immunology, 117, pp.36~52 (2021)[DOI]
- Guo, X., "Identification and characterization of Bacillus subtilis from grass carp(Ctenopharynodon idellus)for use as probiotic additives in aquatic feed", Fish & shellfish immunology, 52, pp.74~84 (2016)[DOI]
- Hao, K., "Effects of dietary administration of Shewanella xiamenensis A-1, Aeromonas veronii A-7, and Bacillus subtilis, single or combined, on the grass carp(Ctenopharyngodon idella)intestinal microbiota", Probiotics and antimicrobial proteins, 9, pp.386~396 (2017)[DOI]
- Hashmi, S., "Production, optimization, and partial purification of alkali-thermotolerant proteases from newly isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12", Processes, 10(6), pp.1050~ (2022)[DOI]
- Janda, J. M., "The changing face of the family Enterobacteriaceae(Order : "Enterobacterales") : New members, taxonomic issues, geographic expansion, and new diseases and disease syndromes", Clinical microbiology reviews, 34(2), pp.10~1128 (2021)[DOI]
- Kim, D. H., "Dietary effect of Bacillus subtilis MD-02 on Innate Immune Response and Disease Resistance in Olive Flounder, Paralichthys olivaceus", Microbiol. Biotechnol. Lett, 47(1), pp.132~138 (2019)[DOI]
- Kim, H. W., "The antibiotic resistance of Lactococcus lactis isolated from the farmed Anguilla japonica", J. Fish Pathol, 36(1), pp.107~116 (2023)[DOI]
- Kim, H. W., "Characterization of Photobacterium sp. YW2207isolated from rainbow trout (Oncorhynchus mykiss)raised in a fresh water farm in South Korea", J. Fish Pathol, 36(2), pp.251~261 (2023)[DOI]
- Kuebutornye, F. K., "A review on the application of Bacillus as probiotics in aquaculture", Fish & shellfish immunology, 87, pp.820~828 (2019)[DOI]
- Lee, S. H., "Comparative evaluation of dietary probiotics Bacillus subtilis WB60 and Lactobacillus plantarum KCTC3928 on the growth performance, immunological parameters, gut morphology and disease resistance in Japanese eel, Anguilla japonica", Fish & shellfish immunology, 61, pp.201~210 (2017)[DOI]
- Logan, N. A., "Identification of Bacillus strains using the API system", Microbiology, 130(7), pp.1871~1882 (1984)[DOI]
- Mabrok, M., "Enterobacter cloacae as a re-emerging pathogen affecting mullets (Mugil spp.): Pathogenicity testing, LD50, antibiogram, and encoded antimicrobial resistance genes", Aquaculture, 583, pp.740619~ (2024)[DOI]
- Nair, A. V., "Evaluation of in vitro and in vivo potential of Bacillus subtilis MBTDCMFRI Ba37 as a candidate probiont in fish health management", Microbial Pathogenesis, 152, pp.104610~ (2021)[DOI]
- Nayak, S. K., "Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis", Reviews in Aquaculture, 13(2), pp.862~906 (2021)[DOI]
- Pereira, C., "Bacteriophages in the control of Aeromonas sp. in aquaculture systems: an integrative view", Antibiotics, 11(2), pp.163~ (2022)[DOI]
- Ren, X., "Antagonistic activity and protective effect of a Bacillus subtilis isolate against fish pathogen Edwardsiella piscicida", Fisheries science, 85, pp.1011~1018 (2019)[DOI]
- Rohani, M. F., "Probiotics, prebiotics and synbiotics improved the functionality of aquafeed : Upgrading growth, reproduction, immunity and disease resistance in fish", Fish & shellfish immunology, 120, pp.569~589 (2022)[DOI]
- Zhao, C., "A novel disease(water bubble disease)of the giant freshwater prawn Macrobrachium rosenbergii caused by Citrobacter freundii : antibiotic treatment and effects on the antioxidant enzyme activity and immune responses", Antioxidants, 11(8), pp.1491~ (2022)[DOI]
- Zokaeifar, H., "Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei", Fish & shellfish immunology, 36(1), pp.68~74 (2014)[DOI]
- Zuenko, V. A., "Effect of Bacillus subtilis in feed probiotic on the digestion of fish cultured in cages", Journal of ichthyology, 57, pp.152~157 (2017)[DOI]
