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Prevalence of Human Papillomavirus Infection and Genotype Distribution Determined via Real-Time PCR in a Korean Medical Check-up Population

김재경|김종완|전재식
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2018.06|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제46권 제2호|pp. 171-179

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참고문헌

키워드

HPVHuman papillomavirusgenotypingreal-time PCR
주제분류생물학

초록

Human papillomavirus (HPV) plays a critical role in the development of cervical carcinoma. This study analyzed the efficiency of multiplex real-time PCR in detecting and identifying HPV genotypes in samples from women who visited a Korean hospital for checkups. Cervical swab specimens were obtained from women who attended a checkup at the Health Improvement Center of Hospital in Dankook University Cheonan, South Korea and were referred for an HPV genotyping test between January and September 2014. A total of 1703 cervical swab specimens were collected consecutively during this period. PCR results were compared with those of the traditional cytological assay for the same population. Among the 1,703 specimens, 19.91% were HPV positive, of which 14.50% indicated a single infection and 5.40% indicated multiple infections. However, cytology identified only 2.52% of positive cases, including 1.23% cases of atypical squamous cells of undetermined significance, 1% of low grade squamous intra-epithelial lesion, and 0.29% of high grade squamous intra-epithelial lesion. The rate of high-risk and low-risk HPV in the abnormal cytology group was 48 and 23, respectively, and 274 and 136 in the normal group, respectively. HPV types 56, 52, 43 were the most prevalent in that order. Our results confirm the efficiency of the HPV DNA assay for the detection of 28 different HPV genotypes with reasonable sensitivity. A screening strategy that comprises the HPV DNA assay and cytology would help overcome the low sensitivity of a cytological diagnosis.

Abstract

Human papillomavirus (HPV) plays a critical role in the development of cervical carcinoma. This study analyzed the efficiency of multiplex real-time PCR in detecting and identifying HPV genotypes in samples from women who visited a Korean hospital for checkups. Cervical swab specimens were obtained from women who attended a checkup at the Health Improvement Center of Hospital in Dankook University Cheonan, South Korea and were referred for an HPV genotyping test between January and September 2014. A total of 1703 cervical swab specimens were collected consecutively during this period. PCR results were compared with those of the traditional cytological assay for the same population. Among the 1,703 specimens, 19.91% were HPV positive, of which 14.50% indicated a single infection and 5.40% indicated multiple infections. However, cytology identified only 2.52% of positive cases, including 1.23% cases of atypical squamous cells of undetermined significance, 1% of low grade squamous intra-epithelial lesion, and 0.29% of high grade squamous intra-epithelial lesion. The rate of high-risk and low-risk HPV in the abnormal cytology group was 48 and 23, respectively, and 274 and 136 in the normal group, respectively. HPV types 56, 52, 43 were the most prevalent in that order. Our results confirm the efficiency of the HPV DNA assay for the detection of 28 different HPV genotypes with reasonable sensitivity. A screening strategy that comprises the HPV DNA assay and cytology would help overcome the low sensitivity of a cytological diagnosis.

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관련 논문AI추천

Type-specific prevalence of high-risk human papillomavirus by cervical cytology and age: Data from the health check-ups of 7,014 Korean women김민정, 김선미, 김진주대한산부인과학회 · Obstetrics & Gynecology Science2013 · 제56권 제02호Human Papillomavirus Genotype Distribution Among 18,815 Women in 13 Korean Cities and Relationship With Cervical Cytology Findings조선, 김수영, 나은희, 조한익대한진단검사의학회 · Annals of Laboratory Medicine2017 · 제37권 제5호Prevalence and Genotype Distribution of Cervical Human Papillomavirus DNA in Korean Women: A Multicenter Study최찬, 김동원, 김미진, 김애리, 김영옥, 김인선, 김혜선, 나서희, 박경신, 박용구, 박용욱, 박호성, 서광선, 손진희, 신미경, 오훈규, 윤기중, 윤혜경, 이시내, 이아원, 이효진, 정운원, 홍성란대한병리학회 · Journal of Pathology and Translational Medicine2009 · 제43권 제4호국내 광주, 전남 지역에서 비정상적인 자궁경부 표본과연관된 인 유두종 바이러스의 유전자형 분석박미숙, 조현욱한국보건정보통계학회 · 보건정보통계학회지2013 · 제38권 제2호Investigation of Detected by Recent Various Human Papillomavirus from General Hospital in Seoul Area박창은, 이준범대한임상검사과학회 · 대한임상검사과학회지2016 · 제48권 제3호Genotype Frequency of Human Papillomavirus Determined by PCR and DNA Sequencing in Korean Women김경태, 성혜순, 이경옥, 이혜정, 정나영, 정수진대한임상검사과학회 · 대한임상검사과학회지2006 · 제38권 제2호

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