본문 바로가기
KERD
논문KCI 등재

Anti-Inflammatory and Anti-Fibrotic Activities of Nocardiopsis sp. 13G027 in LipopolysaccharidesInduced RAW 264.7 Macrophages and Transforming Growth Factor Beta-1-Stimulated Nasal Polyp-Derived Fibroblasts

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

Nocardiopsis species produce bioactive compounds, such as antimicrobial and anti-cancer agents and toxins. However, no reports have described their anti-inflammatory and anti-fibrotic effects during nasal polyp (NP) formation. In this study, we investigated whether marine-derived bacterial Nocardiopsis sp. 13G027 exerts anti-inflammatory and anti-fibrotic effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and transforming growth factor (TGF)-β1-induced NP-derived fibroblasts (NPDFs). Nitric oxide (NO) and prostaglandin E2 (PGE2) levels were analyzed. Extract from Nocardiopsis sp. 13G027 significantly inhibited the upregulation of NO and PGE2 in LPS-activated RAW 264.7 macrophages. The expression of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt/PKB) in LPS-induced RAW 264.7 macrophages was evaluated; smooth muscle alpha-actin (α-SMA), collagen type I (Col-1), and fibronectin also phosphorylated small mothers against decapentaplegic (SMAD) 2 and 3 in TGF-β1–stimulated NPDFs. The Nocardiopsis sp. 13G027 extract suppressed the phosphorylation of MAPKs and Akt and the DNA-binding activity of activator protein 1 (AP-1). The expression of pro-fibrotic components such as α- SMA, Col-1, fibronectin, and SMAD2/3 was inhibited in TGF-β1–exposed NPDFs. These findings suggest that Nocardiopsis sp. 13G027 has the potential to treat inflammatory disorders, such as NP formation.

Abstract

Nocardiopsis species produce bioactive compounds, such as antimicrobial and anti-cancer agents and toxins. However, no reports have described their anti-inflammatory and anti-fibrotic effects during nasal polyp (NP) formation. In this study, we investigated whether marine-derived bacterial Nocardiopsis sp. 13G027 exerts anti-inflammatory and anti-fibrotic effects on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and transforming growth factor (TGF)-β1-induced NP-derived fibroblasts (NPDFs). Nitric oxide (NO) and prostaglandin E2 (PGE2) levels were analyzed. Extract from Nocardiopsis sp. 13G027 significantly inhibited the upregulation of NO and PGE2 in LPS-activated RAW 264.7 macrophages. The expression of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt/PKB) in LPS-induced RAW 264.7 macrophages was evaluated; smooth muscle alpha-actin (α-SMA), collagen type I (Col-1), and fibronectin also phosphorylated small mothers against decapentaplegic (SMAD) 2 and 3 in TGF-β1–stimulated NPDFs. The Nocardiopsis sp. 13G027 extract suppressed the phosphorylation of MAPKs and Akt and the DNA-binding activity of activator protein 1 (AP-1). The expression of pro-fibrotic components such as α- SMA, Col-1, fibronectin, and SMAD2/3 was inhibited in TGF-β1–exposed NPDFs. These findings suggest that Nocardiopsis sp. 13G027 has the potential to treat inflammatory disorders, such as NP formation.

참고문헌 (33)

  1. 조정선, "Inhibitory Effect of Delphinidin on Extracellular Matrix Production via the MAPK", NF-κB Pathway in Nasal Polyp-Derived Fibroblasts, 2015, pp.7(3), 276~282[DOI]
  2. Pawankar R, "Nasal polyposis: an update: editorial review", Curr. Opin. Allergy Clin. Immunol, 3, pp.1~6 (2003)
  3. Jung JW, "Naringenin inhibits extracellular matrix production via extracellular signal-regulated kinase pathways in nasal polyp-derived fibroblasts", Phytother. Res, 27, pp.463~467 (2013)
  4. Cho JS, "Inhibitory effect of ginsenoside Rg1 on extracellular matrix production via extracellular signal-regulated protein kinase", activator protein 1 pathway in nasal polyp-derived fibroblasts, 2012, pp.237, 663~669
  5. Bennur T, "Nocardiopsis species: incidence, ecological roles and adaptations", Microbiol. Res, 174, pp.33~47 (2015)
  6. Sun HH, "Methylpendolmycin an indolelactam from a Nocardiopsis sp", J. Nat. Prod, 54, pp.1440~1443 (1991)
  7. Kim JW, "Apoptolidin, a new apoptosis inducer in transformed cells from Nocardiopsis sp", J. Antibiot, 50, pp.628~630 (1997)
  8. Li YQ, "Griseusin D, a new pyranonaphthoquinone derivative from a alkaphilic Nocardiopsis sp", J. Antibiot, 60, pp.757~761 (2007)
  9. Gandhimathi R, "Production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes Nocardiopsis alba MSA10", Bioprocess Biosyst. Eng, 32, pp.825~835 (2009)
  10. Engelhardt K, "Production of a new thiopeptide antibiotic, TP-1161, by a marine Nocardiopsis species", Appl. Environ. Micobiol, 76, pp.4969~4976 (2010)
  11. Ding ZG, "Naphthospironone A : an unprecedented and highly functionalized polycyclic metabolite from an alkalinemine waste extremophile", Chemistry, 16, pp.3902~3905 (2010)
  12. Altschul SF, "Basic local alignment search tool", J. Mol. Biol, 215, pp.403~410 (1990)
  13. Re R, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radic. Biol. Med, 26, pp.1231~1237 (1999)
  14. Schmölz L, "Optimized incubation regime for nitric oxide measurements in murine macrophages using the Griess assay", J. Immunol. Methods, 449, pp.68~70 (2017)
  15. Lee DS, "Antiinhibitory potential of an ethanolic extract of Distromium decumbens on pro-inflammatory cytokine production in Pseudomonas aeruginosa lipopolysaccharide-stimulated nasal polyp-derived fibroblasts", Int. J. Mol. Med, 40, pp.1950~1956 (2017)
  16. Sharma JN, "Role of nitric oxide in inflammatory diseases", Inflammophamacology, 15, pp.252~259 (2007)
  17. Ruhee RT, "Sulforaphane protects cells against lipopolysaccharide-stimulated inflammation in murine macrophages", Antioxidants, 8, pp.577~ (2019)
  18. Kwon DH, "Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-kB, MAPKs and PI3K", Akt pathways and activating Nrf2, Int. J. Mol. Med, pp.2018, 41[DOI]
  19. Zong Y, "Resveratrol inhibits LPS-induced MAPKs activation via activation of the phosphatidylinositol 3-kinase pathway in murine RAW 264.7macrophage cells", PLoS One, 7, pp.e44107~ (2012)
  20. Guha M, "LPS induction of gene expression in human monocytes", Cell. Signal, 13, pp.85~94 (2001)
  21. Hu W, "Apigenin-7-O-β-D-glucuronide inhibits LPS-induced inflammation through the inactivation of AP-1 and MAPK signaling pathways in RAW 264.7 macrophages and protects mice against endotoxin shock", Food Funct, 7, pp.1002~1013 (2016)
  22. Li X, "Anti-inflammatory effects of chloranthalactone B in LPS-stimulated RAW 264.7 cells", Int. J. Mol. Sci, 17, pp.1938~ (2016)
  23. Lin D, "An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes", Molecules, 21, pp.1374~ (2016)
  24. Xu X, "Punicalagin induces Nrf2", HO-1 expression via upregulation of PI3K, Mediat. Inflamm, pp.2015, 2015[DOI]
  25. Kaminska B, "MAPK signaling pathways as molecular targets for anti-inflammatory therapy-from molecular mechanisms to therapeutic benefits", Biochem. Biophys. Acta, 1754, pp.253~262 (2005)
  26. Williams D, "Modulation of the phosphoinositide 3-kinase pathway alters innate resistance to polymicrobial sepsis", J. Immunol, 172, pp.449~456 (2004)
  27. Salaria N, "Inflammatory septal nasal polyp", Iran J. Otorhinolaryngol, 27, pp.319~323 (2015)
  28. Wick G, "The immunology of fibrosis: Innate and adaptive responses", Trends Immunol, 31, pp.110~119 (2010)
  29. Park SK, "IL-25-induced activation of nasal fibroblast and its association with the remodeling of chronic rhinosinusitis with nasal polyposis", PLoS One, 12, pp.e0181806~ (2017)
  30. Shoulders MD, "Collagen structure and stability", Annu. Rev. Biochem, 78, pp.929~958 (2009)
  31. Ryu NH, "Wogonin inhibits transforming growth factor beta1-induced extracellular matrix production via the p38", activator protein 1 signaling pathway in nasal polyp-derived fibroblasts, 2016, pp.30, 128~133
  32. Little SC, "Dual action of TGF-beta1 on nasal-polyp derived fibroblasts", Laryngoscope, 118, pp.320~324 (2008)
  33. Park IH, "Role of reactive oxygen species in transforming growth factor beta1-induced alpha smooth-muscle actin and collagen production in nasal polyp-derived fibroblasts", Int. Arch. Allergy Immunol, 159, pp.278~286 (2012)

관련 논문AI추천

Ethanolic Extract of Chondria crassicaulis Inhibits the Expression of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in LPS-Stimulated RAW 264.7 Macrophages김연계, 김재일, 김형락, 윤나영, 윤호동, 이민섭, 정은지한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제4호Anti-inflammatory Effects of Ethanolic Extracts from Codium fragile on LPS-Stimulated RAW 264.7 Macrophages via Nuclear Factor kappaB Inactivation김연계, 김재일, 김형락, 박명애, 윤나영, 윤호동, 이민섭, 정은지, 조득문, 최지웅한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제4호Anti-Inflammatory Activity of Questinol Isolated from Marine-Derived Fungus Eurotium amstelodami in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages강민철, 전유진, Eun-A Kim, Sung-Myung Kang, Xiudong Yang, Yong Li한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2014 · 제24권 제10호Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages김서영, 김학주, 김현수, 안긴내, 전유진, 지영훈, K. K. Asanka Sanjeewa, Thilina U. Jayawardena, Xiaoting Fu한국수산과학회 · Fisheries and Aquatic Sciences2019 · 제22권 제1호Reconsideration of Classical Antibiotic Lincomycin: Anti-inflammatory Effect in LPS-stimulated RAW 264.7 Cells양은진, 이나리, 현창구한국미생물·생명공학회 · 한국미생물·생명공학회지2020 · 제48권 제3호Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells김은아, 안긴내, 전유진, 지영흔, Ilekkuttige Priyan Shanura Fernando, KALU KAPUGE ASANKA SANJEEWA한국영양학회 · Nutrition Research and Practice2017 · 제11권 제1호

이 간행물의 다른 논문 (15건)