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신규 Pseudomonas sp. MBEL21 균주의Polyhydroxyalkanoates 생산 특성

Isolation and Characteristics of Polyhydroxyalkanoates Producing Pseudomonas sp. MBEL21.

이승환|이상엽|최종일
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2004.06|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제32권 제2호|pp. 123-127

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참고문헌

주제분류생물학

초록

토양으로부터 oleic acid를 탄소원으로 이용하여 MCL-PHA의 생산능이 우수한 균주를 분리하여 Pseudomonas sp. MBEL21이라 명명하였다. Pseudomonas sp. MBEL21의 PHA 함량을 증가시키기 위하여 여러 가지 필수 영양분의 제한 조건에서 배양한 결과, 인산이 제한된 조건에서 가장 높은 함량의 PHA를 축적하였다. 고농도의 MCL-PHA 생산을 위한 Pseudomonas sp. MBEL21의 유가식 배양 전략을 개발하여 28.1 wt%의 함량으로 23 g/L MCL-PHA를 생산하였다. 또한, 분리된 Pseudomonas sp. MBEL21을 olive oil을 탄소원으로 이용하여 배양한 결과 9.3 wt%의 PHA가 축적되어졌으며, MCL-hydroxyalkanoate와 함께 3-hydroxy-butyrate의 단량체들로 이루어진 PHA가 확인되어졌다. 이러한 결과들은 분리된 신규 Pseudomonas sp. MBEL21이 저가의 olive oil로부터 생분해성 탄성체의 응용분야를 갖는 MCL-PHA를 효율적으로 생산할 수 있다는 것을 보여준다.

Abstract

Pseudomonas sp. MBEL21 was newly isolated from soil samples and found to accumulate medium-chain-length polyhydroxyalkanoates (MCL-PHAs) using oleic acid as a sole carbon source. Among the various nutrient limiting conditions examined, including nitrogen, sulfur and phosphorus, only phosphorus limitation supported the accumulation of MCL-PHAs up to 15 wt% of dry cell weight in flask cultures. MCL-PHAs produced by Pseudomonas sp. MBEL21 was mainly composed of 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecanoate and 3-hydroxy-5-cis-tetradecenoate. Fed-batch culture of Pseudomonas sp. MBEL21 by novel feeding strategies based on cell growth characteristics was carried out under phosphorus limitation using oleic acid as a sole carbon source. The final cell concentration and PHA content of 82 g/L and 28 wt%, respectively, were obtained. Furthermore, PHA consisted of MCL-hydroxyalkanoates and 3-hydroxybutyrate could be produced using olive oil as a sole carbon source.

참고문헌 (22)

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