우모분해세균 Bacillus megaterium F7-1에 의한 단백질 분해효소 생산에 영향을 미치는 배양조건
Cultural Conditions for Protease Production by a Feather-Degrading Bacterium, Bacillus megaterium F7-1
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Abstract
The effects of inorganic salts and feather concentrations on protease production by Bacillus megaterium F7-1 were investigated. Protease production was dependent on the presence of phosphates in the medium. Supplementation of medium with calcium ion slightly increased protease production. The highest protease production was obtained at 1.4% feather. The optimal medium contained 0.2% glucose, 0.8% skim milk, 0.06% K2HPO4%, 0.04% KH2PO4, 0.06% NaCl, 0.03% MgCl2·6H2O, 0.002% CaCl2·2H2O and 1.4% whole feather.By using this optimized medium, increased production of the protease was achieved compared with the cases of using basal medium.
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